beta COP (COPB1) (NM_016451) Human Untagged Clone

CAT#: SC108296

COPB1 (untagged)-Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: beta COP
• Synonyms: BARMACS; COPB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108296 sequence for NM_016451 edited (data generated by NextGen Sequencing)
  ATGACGGCGGCTGAGAACGTATGCTACACGTTAATTAACGTGCCAATGGATTCAGAACCA
  CCATCTGAAATTAGCTTAAAAAATGATCTAGAAAAAGGAGATGTAAAGTCAAAGACTGAA
  GCTTTGAAGAAAGTAATCATTATGATTCTGAATGGTGAAAAACTTCCTGGACTTCTGATG
  ACCATCATTCGTTTTGTGCTACCTCTTCAGGATCACACTATCAAGAAATTACTTCTGGTA
  TTTTGGGAAATTGTTCCTAAAACAACTCCAGATGGGAGACTTTTACATGAGATGATCCTT
  GTATGTGATGCATACAGAAAGGATCTTCAACATCCTAATGAATTTATTCGAGGATCTACT
  CTTCGTTTTCTTTGCAAATTGAAAGAAGCAGAATTGCTAGAACCTTTAATGCCAGCTATT
  CGTGCATGTTTGGAGCATCGACACAGCTATGTTAGAAGAAATGCTGTTTTGGCCATCTAT
  ACCATCTATAGAAATTTTGAACATCTTATACCTGATGCTCCTGAACTGATACATGATTTT
  CTGGTGAATGAGAAGGATGCAAGTTGCAAAAGGAATGCATTTATGATGCTAATTCATGCA
  GATCAGGATCGAGCTTTGGATTACTTAAGTACTTGCATTGATCAAGTTCAAACATTTGGA
  GACATTCTGCAGCTGGTTATTGTTGAACTGATTTATAAGGTCTGTCATGCTAATCCATCA
  GAAAGAGCTCGTTTTATTCGCTGCATCTATAACTTATTACAGTCATCCAGCCCTGCTGTA
  AAATATGAAGCTGCTGGGACATTAGTGACACTCTCTAGTGCACCAACTGCAATCAAGGCT
  GCTGCTCAGTGTTACATTGATTTAATTATTAAGGAGAGCGACAACAATGTAAAACTCATA
  GTTTTGGATCGCTTGATAGAATTAAAAGAGCATCCTGCTCATGAACGAGTACTACAGGAT
  CTGGTTATGGATATCCTAAGAGTATTGAGCACACCAGACTTAGAAGTACGAAAGAAAACT
  CTGCAGTTAGCACTGGATCTTGTCTCTTCTAGAAATGTTGAAGAGCTGGTTATTGTCCTG
  AAGAAGGAAGTGATAAAAACAAATAATGTGTCTGAGCATGAAGATACTGACAAATACAGA
  CAACTCCTAGTGCGAACATTGCATTCCTGTTCTGTCCGATTTCCAGATATGGCTGCAAAT
  GTTATTCCTGTGTTAATGGAATTTCTCAGTGACAACAACGAAGCAGCAGCTGCTGATGTC
  TTGGAGTTTGTTCGTGAAGCCATTCAGCGCTTTGATAACCTGAGAATGCTTATTGTTGAG
  AAGATGCTTGAAGTCTTTCATGCTATTAAATCTGTCAAGATTTACCGAGGAGCATTATGG
  ATCCTGGGAGAATACTGTAGTACCAAGGAAGACATTCAGAGTGTGATGACTGAGATCCGC
  AGGTCCCTTGGAGAGATCCCAATTGTAGAGTCAGAAATAAAGAAAGAAGCTGGTGAATTA
  AAACCTGAAGAAGAAATAACTGTAGGGCCAGTTCAGAAATTGGTTACTGAAATGGGTACC
  TATGCAACTCAGAGTGCCCTTAGCAGTTCTAGACCCACCAAGAAAGAGGAAGACAGACCT
  CCCTTGAGAGGATTCCTTCTGGATGGAGATTTCTTTGTTGCTGCCTCCCTTGCCACAACT
  CTGACCAAGATTGCATTGCGCTATGTAGCTTTGGTTCAGGAGAAGAAAAAGCAAAATTCT
  TTTGTTGCTGAGGCTATGTTGCTCATGGCTACTATCCTGCATTTGGGAAAATCCTCTCTT
  CCTAAGAAGCCAATTACTGATGATGATGTGGATCGAATTTCCCTGTGCCTCAAGGTCTTG
  TCTGAATGTTCACCTTTAATGAATGACATTTTCAATAAGGAATGCAGACAGTCCCTTTCT
  CACATGTTATCTGCTAAACTAGAAGAAGAGAAATTATCCCAAAAGAAAGAATCTGAAAAG
  AGGAATGTGACAGTACAGCCTGATGACCCCATTTCCTTCATGCAACTAACTGCTAAGAAT
  GAAATGAACTGCAAGGAAGATCAGTTTCAGCTGAGTTTACTGGCAGCAATGGGTAACACA
  CAGAGGAAAGAGGCAGCAGATCCCCTAGCATCTAAACTTAACAAGGTCACCCAATTGACA
  GGTTTCTCAGATCCTGTATATGCAGAAGCTTACGTTCATGTCAACCAATATGATATTGTC
  CTGGATGTACTTGTTGTGAACCAAACCAGTGATACTTTGCAGAATTGCACATTAGAACTA
  GCTACACTAGGGGATCTGAAACTTGTGGAAAAGCCGTCTCCTTTGACTCTTGCTCCTCAT
  GACTTCGCAAATATTAAAGCTAACGTCAAAGTAGCATCAACAGAAAATGGAATAATTTTT
  GGTAATATAGTTTATGATGTCTCTGGAGCAGCAAGTGACAGAAATTGTGTGGTTCTCAGT
  GATATTCACATCGACATCATGGACTATATCCAGCCTGCAACTTGCACTGATGCAGAATTC
  CGTCAGATGTGGGCCGAATTTGAATGGGAAAACAAAGTGACAGTTAACACCAACATGGTT
  GATTTAAATGACTACTTACAGCACATATTAAAGTCAACCAATATGAAATGCCTGACTCCA
  GAAAAGGCCCTTTCTGGTTACTGTGGCTTTATGGCAGCCAACCTTTATGCTCGTTCCATA
  TTTGGTGAAGATGCACTTGCAAATGTCAGCATTGAGAAGCCAATTCACCAGGGACCAGAT
  GCTGCTGTTACCGGCCATATAAGAATTCGTGCAAAGAGCCAGGGAATGGCCTTAAGTCTT
  GGAGATAAAATCAACTTGTCACAGAAGAAAACTAGTATATAA
  
  Clone variation with respect to NM_016451.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016451 unedited
  CGGTCAACATTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGCCCCCA
  GTCCCAAAGGCGTGATTTTCTGGCATCCTTAAATCTTGTGTCAAGGATTGGTTATAATAT
  AACCAGAAACCATGACGGCGGCTGAGAACGTATGCTACACGTTAATTAACGTGCCAATGG
  ATTCAGAACCACCATCTGAAATTAGCTTAAAAAATGATCTAGAAAAAGGAGATGTAAAGT
  CAAAGACTGAAGCTTTGAAGAAAGTAATCATTATGATTCTGAATGGTGAAAAACTTCCTG
  GACTTCTGATGACCATCATTCGTTTTGTGCTACCTCTTCAGGATCACACTATCAAGAAAT
  TACTTCTGGTATTTTGGGAAATTGTTCCTAAAACAACTCCAGATGGGAGACTTTTACATG
  AGATGATCCTTGTATGTGATGCATACAGAAAGGATCTTCAACATCCTAATGAATTTATTC
  GAGGATCTACTCTTCGTTTTCTTTGCAAATTGAAAGAAGCAGAATTGCTAGAACCTTTAA
  TGCCAGCTATTCGTGCATGTTTGGAGCATCGACACAGCTATGTTAGAAGAAATGCTGTTT
  TGGCCATCTATACCATCTATAGAAATTTTGAACATCTTATACCTGATGCTCCTGAACTGA
  TACATGATTTTCTGGTGAATGAGAAGGATGCAAGTTGCAAAAGGAATGCATTTATGATGC
  TAATTCATGCAGATCAGGATCGAGCTTTGGATTACTTAAGTACTTGCATTGATCAAGTTC
  AAACATTTGGAGACATTCTGCAGCTGGNTATTGGTTGACTGATTTTATAAGGTCTGTCAT
  GCTAATCCATCAGAAGAAGCTCGTTTTATTCGCTGCATCTATTACTTATTACAGTCATCC
  CAGCCTGCTGTAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016451 unedited
  GTCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTATGAATAGATCTGTTT
  ATTGTACTGTGAAAAGGGTCTTGGTACATGAAACATTATATACTTTGAGGACAGCATTCA
  GAACTGTTAAGACAAAAGACAAACTATAATTTTAAACTCAATCTTGATTTAAGGGAAAGG
  CTATAAATTATTGGCTGAAAAGTATTCAGCATGAACTCAGATTCTATATAAGCATGAAAG
  AGTACAAAAGATGTTGGAGTCCAGTAAGCCCATACCTAAATTAACTGTAAAGCTTCAAGG
  ACTTTTTGTTTATTTTTATATACTAGTTTTCTTCTGTGACAAGTTGATTTTATCTCCAAG
  ACTTAAGGCCATTCCCTGGCTCTTTGCACGAATTCTTATATGGCCGGTAACAGCAGCATC
  TGGTCCCTGGTGAATTGGCTTCTCAATGCTGACATTTGCAAGTGCATCTTCACCAAATAT
  GGAACGAGCATAAAGGTTGGCTGCCATAAAGCCACAGTAACCAGAAAGGGCCTTTTCTGG
  AGTCAGGCATTTCATATTGGCTGACTTTAATATGTGCTGCAAGTACCCATTTAAATCAAC
  CATGTCGGTGGCAACCTGTACCTTTGTTCCCCCATTTCAAATCTCGGCCCACCATTGACA
  CGAATTTTGCATTACTCGCAAGTTCGCATGGCTGGAATATTCCCCCCTGATGTCCAATGT
  GAACTAATCACTGAAAACCCAACCACAATTTCTTGGCCCTTTGCTTGCTCCCAGAGACAA
  TTTTAAACCCTTTTTACCCAAAATTTATTTCCCATTTTCTGGTCGATGCCCACCTCCCCC
  CTTACCCTTTAATTTTTGCCGAATTCCTTAAGGGCCACAAAGTCCAAGGGAAAGAGCCTT
  TCCCACAAATTTCCAACACCCCTTCTGTACCTCATATCTAATGCGCACATCCTCCAAACC
  CCACCGGTTCGGTTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016451
• Insert Size: 3800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016451.3, NP_057535.1
• RefSeq Size: 3528 bp
• RefSeq ORF: 2862 bp
• Locus ID: 1315
• UniProt ID: P53618
• Cytogenetics: 11p15.2
• Domains: Adaptin_N
• Gene Summary: This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.