Casein Kinase 2 beta (CSNK2B) (NM_001320) Human Untagged Clone

CAT#: SC108248

CSNK2B (untagged)-Human casein kinase 2, beta polypeptide (CSNK2B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Casein Kinase 2 beta
• Synonyms: CK2B; CK2N; Ckb1; Ckb2; CSK2B; G5A; POBINDS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001320 edited
  CCCCCACCCCACTTCGCCTGCCGCGGTCGGGTCCGCGGCCTGCGCTGTAGCGGTCGCCGC
  CGTTCCCTGGAAGTAGCAACTTCCCTACCCCACCCCAGTCCTGGTCCCCGTCCAGCCGCT
  GACGTGAAGATGAGCAGCTCAGAGGAGGTGTCCTGGATTTCCTGGTTCTGTGGGCTCCGT
  GGCAATGAATTCTTCTGTGAAGTGGATGAAGACTACATCCAGGACAAATTTAATCTTACT
  GGACTCAATGAGCAGGTCCCTCACTATCGACAAGCTCTAGACATGATCTTGGACCTGGAG
  CCTGATGAAGAACTGGAAGACAACCCCAACCAGAGTGACCTGATTGAGCAGGCAGCCGAG
  ATGCTTTATGGATTGATCCACGCCCGCTACATCCTTACCAACCGTGGCATCGCCCAGATG
  TTGGAAAAGTACCAGCAAGGAGACTTTGGTTACTGTCCTCGTGTGTACTGTGAGAACCAG
  CCAATGCTTCCCATTGGCCTTTCAGACATCCCAGGTGAAGCCATGGTGAAGCTCTACTGC
  CCCAAGTGCATGGATGTGTACACACCCAAGTCATCAAGACACCATCACACGGATGGCGCC
  TACTTCGGCACTGGTTTCCCTCACATGCTCTTCATGGTGCATCCCGAGTACCGGCCCAAG
  AGACCTGCCAACCAGTTTGTGCCCAGGCTCTACGGTTTCAAGATCCATCCGATGGCCTAC
  CAGCTGCAGCTCCAAGCCGCCAGCAACTTCAAGAGCCCAGTCAAGACGATTCGCTGATTC
  CCTCCCCCACCTGTCCTGCAGTCTTTGACTTTTCCTTTCTTTTTTGCCACCCTTTCAGGA
  ACCCTGTATGGTTTTTAGTTTAAATTAAAGGAGTCGTTATCGTGGTGGGAATATGAAATA
  AAGTAGAAGAAAAGGCCAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001320 unedited
  GCAACCCAGACTTTACTATAGGGCCGGCCCGCGTAATTCGGCACGAGGGCCCCCACCCCA
  CTTCGCCTGCCGCGGTCGGGTCCGCGGCCTGCGCTGTAGCGGTCGCCGCCGTTCCCTGGA
  AGTAGCAACTTCCCTACCCCACCCCAGTCCTGGTCCCCGTCCAGCCGCTGACGTGAAGAT
  GAGCAGCTCAGAGGAGGTGTCCTGGATTTCCTGGTTCTGTGGGCTCCGTGGCAATGAATT
  CTTCTGTGAAGTGGATGAAGACTACATCCAGGACAAATTTAATCTTACTGGACTCAATGA
  GCAGGTCCCTCACTATCGACAAGCTCTAGACATGATCTTGGACCTGGAGCCTGATGAAGA
  ACTGGAAGACAACCCCAACCAGAGTGACCTGATTGAGCAGGCAGCCGAGATGCTTTATGG
  ATTGATCCACGCCCGCTACATCCTTACCAACCGTGGCATCGCCCAGATGTTGGAAAAGTA
  CCAGCAAGGAGACTTTGGTTACTGTCCTCGTGTGTACTGTGAGAACCAGCCAATGCTTCC
  CATTGGCCTTTCAGACATCCCAGGTGAAGCCATGGTGAAGCTCTACTGCCCCAAGTGCAT
  GGATGTGTACACACCCCAGTCATCAAGACACCATCACACGGATGCCGCCTACTTCGGCAC
  TGGTTTCCCCTCACATGCTCTTCATGGTGCATCCCGAGTACCGGCCCAAGAGACCTGCCA
  CCCAGTTGTGCCCCAGCTCTACGGTTTTAAGAACCATTCGATGGCCTACCAGCTGCAGCT
  TCAAGCCCGCAGACACTTAAGAGCCCAGTACAGACGATTCGCTTGATCCCTCCCCCACCT
  GGCCTGCAGACCTTTGACCTTTCCCTTTCTTTCTTGCCACCCTTTCAGGACCCCTGCAGG
  GGTTTAAAGTAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001320 unedited
  GGGGGCCAAGNNNNGTACCATTTTNNNCNNNGNGGTCGTGGNACCCGGACCGCATTTTAN
  GATCGNATTTTTTTTTTTTTTTTTTGGCCTTTTCTTCTACTTTATTCATATTCCCACCAC
  GATAACGACTCCTTTAATTTAAACTAAAAACCATACAGGGGTTCCTTGAAGGGTGGCAAA
  AAAGAAAGGAAAAGTCAAAGACTGCAGGACAGGTGGGGGAGGGAATCAGCGAATCGTCTT
  GACTGGGCTCTTGAAGTTGCTGGCGGCTTGGAGCTGCAGCTGGTAGGCCATCGGATGGAT
  CTTGAAACCGTAGAGCCTGGGCACAAACTGGTTGGCAGGTCTCTTGGGCCGGTACTCGGG
  ATGCACCATGAAGAGCATGTGAGGGAAACCAGTGCCGAAGTAGGCGCCATCCGTGTGATG
  GTGTCTTGATGACTTGGGTGTGTACACATCCATGCACTTGGGGCAGTAGAGCTTCACCAT
  GGCTTCACCTGNGATGTCTGAAAGGCCAATGGGAAGCATTGGCTGGTTCTCACAGTACAC
  ACGAGGACAGTAACCAAAGTCTCCTTGCTGGTACTTTTCCAACATCTGGGCGATGCCACG
  GTTGGTAAGGATGTANCGGGCGTGGATCAATCCATAAAGCATCTCGGCTGCCTGCTCAAT
  CAGGTCACTCTGGGTTGGGGNTGTCTTTCCAGTTTTTCATCAGGCTCCAGGTCCAAGATC
  ATGTCTAGAGCTTGTCGATAGTGAGGACCTGCTCATTGAGTCCAGTAAGAATAAATTTGC
  CCTGGAGGAAGCCTCATCCACTTACAGAAGAAATCCATTGCCCGGGACCCACCGGACCCN
  GAATCCAGAACCCTCTTTGACCTGGTATTTTACGTTAAGGTTGGACGGGAACAGGACTGG
  GTGGGGTAGGGAAATTGTACTCCAGGAAAGGGGGGAACCGTAAAGGAAGGCCCGGACCCA
  ACCCGGAAGCAAAGGGGGGGGGCCTTTGCCCAATTCGGGCCCCCTTATGAAACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001320
• Insert Size: 980 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001320.5, NP_001311.3
• RefSeq Size: 1128 bp
• RefSeq ORF: 648 bp
• Locus ID: 1460
• UniProt ID: P67870
• Cytogenetics: 6p21.33
• Domains: CK_II_beta
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Tight junction, Wnt signaling pathway
• Gene Summary: This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.