TIM 3 (HAVCR2) (NM_032782) Human Untagged Clone

CAT#: SC108129

HAVCR2 (untagged)-Human hepatitis A virus cellular receptor 2 (HAVCR2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TIM 3
• Synonyms: CD366; HAVcr-2; KIM-3; SPTCL; Tim-3; TIM3; TIMD-3; TIMD3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032782 edited
  GAATTCGGCACGAGGGTTTTCTTCCACTGAAGTATTGCTTCCTCTTTGTTGATATCACAG
  GACAGACATCAGAACACTTACAGGATGTGTGTAGTGTGGCATGACAGAGAACTTTGGTTT
  CCTTTAATGTGACTGTAGACCTGGCAGTGTTACTATAAGAATCACTGGCAATCAGACACC
  CGGGTGTGCTGAGCTAGCACTCAGTGGGGGCGGCTACTGCTCATGTGATTGTGGAGTAGA
  CAGTTGGAAGAAGTACCCAGTCCATTTGGAGAGTTAAAACTGTGCCTAACAGAGGTGTCC
  TCTGACTTTTCTTCTGCAAGCTCCATGTTTTCACATCTTCCCTTTGACTGTGTCCTGCTG
  CTGCTGCTGCTACTACTTACAAGGTCCTCAGAAGTGGAATACAGAGCGGAGGTCGGTCAG
  AATGCCTATCTGCCCTGCTTCTACACCCCAGCCGCCCCAGGGAACCTCGTGCCCGTCTGC
  TGGGGCAAAGGAGCCTGTCCTGTGTTTGAATGTGGCAACGTGGTGCTCAGGACTGATGAA
  AGGGATGTGAATTATTGGACATCCAGATACTGGCTAAATGGGGATTTCCGCAAAGGAGAT
  GTGTCCCTGACCATAGAGAATGTGACTCTAGCAGACAGTGGGATCTACTGCTGCCGGATC
  CAAATCCCAGGCATAATGAATGATGAAAAATTTAACCTGAAGTTGGTCATCAAACCAGCC
  AAGGTCACCCCTGCACCGACTCTGCAGAGAGACTTCACTGCAGCCTTTCCAAGGATGCTT
  ACCACCAGGGGACATGGCCCAGCAGAGACACAGACACTGGGGAGCCTCCCTGATATAAAT
  CTAACACAAATATCCACATTGGCCAATGAGTTACGGGACTCTAGATTGGCCAATGACTTA
  CGGGACTCTGGAGCAACCATCAGAATAGGCATCTACATCGGAGCAGGGATCTGTGCTGGG
  CTGGCTCTGGCTCTTATCTTCGGCGCTTTAATTTTCAAATGGTATTCTCATAGCAAAGAG
  AAGATACAGAATTTAAGCCTCATCTCTTTGGCCAACCTCCCTCCCTCAGGATTGGCAAAT
  GCAGTAGCAGAGGGAATTCGCTCAGAAGAAAACATCTATACCATTGAAGAGAACGTATAT
  GAAGTGGAGGAGCCCAATGAGTATTATTGCTATGTCAGCAGCAGGCAGCAACCCTCACAA
  CCTTTGGGTTGTCGCTTTGCAATGCCATAGATCCAACCACCTTATTTTTGAGCTTGGTGT
  TTTGTCTTTTTCAGAAACTATGAGCTGTGTCACCTGACTGGTTTTGGAGGTTCTGTCCAC
  TGCTATGGAGCAGAGTTTTCCCATTTTCAGAAGATAATGACTCACATGGGAATTGAACTG
  GGACCTGCACTGAACTTAAACAGGCATGTCATTGCCTCTGTATTTAAGCCAACAGAGTTA
  CCCAACCCAGAGACTGTTAATCATGGATGTTAGAGCTCAAACGGGCTTTTATATACACTA
  GGAATTCTTGACGTGGGGTCTCTGGAGCTCCAGGAAATTCGGGCACATCATATGTCCATG
  AAACTTCAGATAAACTAGGGAAAACTGGGTGCTGAGGTGAAAGCATAACTTTTTTGGCAC
  AGAAAGTCTAAAGGGGCCACTGATTTTCAAAGAGATCTGTGATCCCTTTTTGTTTTTTGT
  TTTTGAGATGGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAATGGCACAATCTCGGCTC
  ACTGCAAGCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTGGCTG
  GGATTACAGGCATGCACCACCATGCCCAGCTAATTTGTTGTATTTTTAGTAGAGACAGGG
  TTTCACCATGTTGGCCAGTGTGGTCTCAAACTCCTGACCTCATGATTTGCCTGCCTCGGC
  CTCCCAAAGCACTGGGATTACAGGCGTGAGCCACCACATCCAGCCAGTGATCCTTAAAAG
  ATTAAGAGATGACTGGACTAGGTCTACCTTGATCTTGAAGATTCCCTTGGAATGTTGAGA
  TTTAGGCTTATTTGAGCACTACCTGCCCAACTGTCAGTGCCAGTGCATAGCCCTTCTTTT
  GTCTCCCTTATGAAGACTGCCCTGCAGGGCTGAGATGTGGCAGGAGCTCCCAGGGAAAAA
  GGAAGTGCATTTGATTGGTGTGTATTGGCCAAGTTTTGCTTGTTGTGTGCTTGAAAGAAA
  ATATCTCTGACCAACTTCTGTATTCGTGGACCAAACTGAAGCTATATTTTTCACAGAAGA
  AGAAGCAGTGACGGGGACACAAATTCTGTTGCCTGGTGGAAAGAAGGCAAAGGCCTTCAG
  CAATCTATATTACCAGCGCTGGATCCTTTGACAGAGAGTGGTCCCTAAACTTAAATTTCA
  AGACGGTATAGGCTTGATCTGTCTTGCTTATTGTTGCCCCCTGCGCCTAGCACAATTCTG
  ACACACAATTGGAACTTACTAAAAATTTTTTTTTACTGTTAAAAAAAAAAAAAAAAAACT
  CGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032782 unedited
  GTTCAGATTTGTATACGACTCATATAGGGCGGCCGCGAATCGGCACGAGGGTTTTCTTCC
  ACTGAAGTATTGCTTCCTCTTTGTTGATATCACAGGACAGACATCAGAACACTTACAGGA
  TGTGTGTAGTGTGGCATGACAGAGAACTTTGGTTTCCTTTAATGTGACTGTAGACCTGGC
  AGTGTTACTATAAGAATCACTGGCAATCAGACACCCGGGTGTGCTGAGCTAGCACTCAGT
  GGGGGCGGCTACTGCTCATGTGATTGTGGAGTAGACAGTTGGAAGAAGTACCCAGTCCAT
  TTGGAGAGTTAAAACTGTGCCTAACAGAGGTGTCCTCTGACTTTTCTTCTGCAAGCTCCA
  TGTTTTCACATCTTCCCTTTGACTGTGTCCTGCTGCTGCTGCTGCTACTACTTACAAGGT
  CCTCAGAAGTGGAATACAGAGCGGAGGTCGGTCAGAATGCCTATCTGCCCTGCTTCTACA
  CCCCAGCCGCCCCAGGGAACCTCGTGCCCGTCTGCTGGGGCAAAGGAGCCTGTCCTGTGT
  TTGAATGTGGCAACGTGGTGCTCAGGACTGATGAAAGGGATGTGAATTATTGGACATCCA
  GATACTGGCTAAATGGGGATTTCCGCANAGGAGATGTGTCCCTGACCATAGAGAATGTGA
  CTCTAGCAGACAGTGNGATCTACTGCTGCCGGATCCAAATCCCAGGCATAATGAATGATG
  AANAATTTAACCTGAAGTTGGTCATCANACCAGCCAAGGTCACCCCTGCACCGACTCTGC
  AGAGAGACTTCACTGCAGCCTTTCCAAGGATGCTTACCACCAGGGGACATGGCCCAGCAG
  AACACAGACACTGGGAGCCCTCCTGATATAATCTACACAATATCACATTGGCCATGAGTT
  ACGGACTCTAGATGGCCATGACTACGGNACTCTGGAGCACATCAGATAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032782 unedited
  TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAACAGTAAAAA
  AAATTTTTAGTAAGTTCCAATTGTGTGTCAGAATTGTGCTAGGCGCAGGGGGCAACAATA
  AGCAAGACAGATCAAGCCTATACCGTCTTGAAATTTAAGTTTAGGGACCACTCTCTGTCA
  AAGGATCCAGCGCTGGTAATATAGATTGCTGAAGGCCTTTGCCTTCTTTCCACCAGGCAA
  CAGAATTTGTGTCCCCGTCACTGCTTCTTCTTCTGTGAAAAATATAGCTTCAGTTTGGTC
  CACGAATACAGAAGTTGGTCAGAGATATTTTCTTTCAAGCACACAACAAGCAAAACTTGG
  CCAATACACACCAATCAAATGCACTTCCTTTTTCCCTGGGAGCTCCTGCCACATCTCAGC
  CCTGCAGGGCAGTCTTCATAAGGGAGACAAAAGAAGGGCTATGCACTGGCACTGACAGTT
  GGGCAGGTAGTGCTCAAATAAGCCTAAATCTCAACATTCCAAGGGAATCTTCAAGATCAA
  GGTAGACCTAGTCCAGTCATCTCTTAATCTTTTAAGGATCACTGGCTGGATGTGGTGGCT
  CACGCCTGTAATCCCAGTGCTTTGGGAGGCCGAGGCAGGCAAATCATGAGGTCAGGAGTT
  TGAGACCACACTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAATACAACAAATTAGC
  TGGGCATGGTGGTGCATGCCTGTAATCCCAGCCACTCAGGAGGCTGAGGCAGGAGAATCG
  CTTGAACCCAGGAGGGCGAGCTTGCAGTGAGCCGAGATTGTGCCATTNGCACTCAGCCTG
  GGCAACANGAGCAGACTNCATCTCAAAAACAAAAACAAAGGGATCCCAGATCTCTTTGAA
  ATCAGTGCCCCCTTAGACTTCCTGTGCAAAAAGTATGCTTCCACTCAGACCCAGTTTCCC
  TAGTTATCTTGAATCCATGGAACTTGATGGGCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032782
• Insert Size: 2660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032782.3, NP_116171.3
• RefSeq Size: 2338 bp
• RefSeq ORF: 906 bp
• Locus ID: 84868
• UniProt ID: Q8TDQ0
• Cytogenetics: 5q33.3
• Domains: IG
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]