MLKL (NM_152649) Human Untagged Clone

CAT#: SC108101

MLKL (untagged)-Human mixed lineage kinase domain-like (MLKL), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MLKL
• Synonyms: hMLKL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_152649 edited
  ATGGAAAATTTGAAGCATATTATCACCCTTGGCCAGGTCATCCACAAACGGTGTGAAGAG
  ATGAAATACTGCAAGAAACAGTGCCGGCGCCTGGGCCACCGCGTCCTCGGCCTGATCAAG
  CCTCTGGAGATGCTCCAGGACCAAGGAAAGAGGAGCGTGCCCTCTGAGAAGTTAACCACA
  GCCATGAACCGCTTCAAGGCTGCCCTGGAGGAGGCTAATGGGGAGATAGAAAAGTTCAGC
  AATAGATCCAATATCTGCAGGTTTCTAACAGCAAGCCAGGACAAAATACTCTTCAAGGAC
  GTGAACAGGAAGCTGAGTGATGTCTGGAAGGAGCTCTCGCTGTTACTTCAGGTTGAGCAA
  CGCATGCCTGTTTCACCCATAAGCCAAGGAGCGTCCTGGGCACAGGAAGATCAGCAGGAT
  GCAGACGAAGACAGGCGAGCTTTCCAGATGCTAAGAAGAGATAATGAAAAAATAGAAGCT
  TCACTGAGACGATTAGAAATCAACATGAAAGAAATCAAGGAAACTTTGAGGCAGTATTTA
  CCACCAAAATGCATGCAGGAGATCCCGCAAGAGCAAATCAAGGAGATCAAGAAGGAGCAG
  CTTTCAGGATCCCCGTGGATTCTGCTAAGGGAAAATGAAGTCAGCACACTTTATAAAGGA
  GAATACCACAGAGCTCCAGTGGCCATAAAAGTATTCAAAAAACTCCAGGCTGGCAGCATT
  GCAATAGTGAGGCAGACTTTCAATAAGGAGATCAAAACCATGAAGAAATTCGAATCTCCC
  AACATCCTGCGTATATTTGGGATTTGCATTGATGAAACAGTGACTCCGCCTCAATTCTCC
  ATTGTCATGGAGTACTGTGAACTCGGGACCCTGAGGGAGCTGTTGGATAGGGAAAAAGAC
  CTCACACTTGGCAAGCGCATGGTCCTAGTCCTGGGGGCAGCCCGAGGCCTATACCGGCTA
  CACCATTCAGAAGCACCTGAACTCCACGGAAAAATCAGAAGCTCAAACTTCCTGGTAACT
  CAAGGCTACCAAGTGAAGCTTGCAGGATTTGAGTTGAGGAAAACACAGACTTCCATGAGT
  TTGGGAACTACGAGAGAAAAGACAGACAGAGTCAAATCTACAGCATATCTCTCACCTCAG
  GAACTGGAAGATGTATTTTATCAATATGATGTAAAGTCTGAAATATACAGCTTTGGAATC
  GTCCTCTGGGAAATCGCCACTGGAGATATCCCGTTTCAAGGCTGTAATTCTGAGAAGATC
  CGCAAGCTGGTGGCTGTGAAGCGGCAGCAGGAGCCACTGGGTGAAGACTGCCCTTCAGAG
  CTGCGGGAGATCATTGATGAGTGCCGGGCCCATGATCCCTCTGTGCGGCCCTCTGTGGAT
  GAAATCTTAAAGAAACTCTCCACCTTTTCTAAGTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152649 unedited
  CGGCCAAAAACTCGGCACGAGCCGGAGGGCACTGGTCAGAAACAGCCATCCAATGGCTGA
  GTTGAGGGACCCTGCTCAAGTGCAGCTGCAGTGGCCGGGGTTTCCCTCAGGAGGCGGTCG
  TGGGAAAGAAGGTGGAAGAGCGAGCTTTTTGGAACTGTGCACGGGACAGATTGGACGCAC
  ACCCTTCGTGAGGCGCGAAGGCATGGAAAATTTGAAGCATATTATCACCCTTGGCCAGGT
  CATCCACAAACGGTGTGAAGAGATGAAATACTGCAAGAAACAGTGCCGGCGCCTGGGCCA
  CCGCGTCCTCGGCCTGATCAAGCCTCTGGAGATGCTCCAGGACCAAGGAAAGAGGAGCGT
  GCCCTCTGAGAAGTTAACCACAGCCATGAACCGCTTCAAGGCTGCCCTGGAGGAGGCTAA
  TGGGGAGATAGAAAAGTTCAGCAATAGATCCAATATCTGCAGGTTTCTAACAGCAAGCCA
  GGACAAAATACTCTTCAAGGACGTGAACAGGAAGCTGAGTGATGTCTGGAAGGAGCTCTC
  GCTGTTACTTCAGGTTGAGCAACGCATGCCTGTTTCACCCATAAGCCAAGGAGCGTCCTG
  NGCACAGGAAGATCAGCAGGATGCAGACGAAGACAGGCGAGCTTTCCAGATGCTAAGAAG
  AGATAATGAAAAAATAGAAGCTTCACTGAGACGATTAGAAATCAACATGAAAGAAATCAA
  GGAAACTTTGAGGCAGTATTTACCACCAAAATGCATGCANGAGATCCCGNCAGAGCANAT
  CAAGGAGATCAAGAAGGAGCAGCTTTCAGGATCCCCGTGGATTCTGCTAAGGGGAAATGA
  AGTCAGCACACTTTATAAAGGAGAATACCACAGAGCTTCAGTGGCCATAAAGTATTCAAA
  AACTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152649 unedited
  GGCGCAATTTTCGGGTTGTTGATTCATCTCTGGATGTGTAGATGGGCAATTTGGGCTGGG
  ATCTGCTTTAGTGGTTCTTCTGGTCTTGATTGGCCTCATTCATGTGTTACCTGCCATGTT
  GGTTGGGCTACTATGACTTGCCCTAGGAAAGCATGTTTCTGCTTCATGTGGTCTCTCATT
  CTCCAGCATGCTCGCTCAACATGTTCATGGCTGGTTTTTGTTTTAACACACATTTATTAT
  GCCTCAGGAAACCCACGCTTGGGACGTGGGCTACAGTCACTTCATCCACATACCATTGAT
  ACAAATCAAGTCACAGCGCCTGCCCGGCATTCTAATGGTTGGTGAAATAGACTTCATTTA
  TACAAGTCCGTAACTGCCTATTCCATTGTTAAGGAGTGTGTATGCACCGGATGAAGCATT
  GGAGAGAGCATCTCGCACACTTAGTGTGCAAAGTCTGGTGTGGCGATACAACTTGGGCCT
  GGGATGTGAAGTGATAAATAAAAAGATAATATCATCAAAAAAGAAACCCAGGACACCGCA
  CGATCGATAGAACAATACGGGGCGCAGAGCTGAGGGAACCACCCAACCAAATCCCAACAC
  TACTGCATGAGCTCGGCGAGCCGACCCGCGCCGCCTGGCCGAGCCGGCCGGGCGCCGGGG
  GAGGCAGGAATCGGACGATGCGCCAGCGACCAAACAGCAAGACCTCCCCCTACACACCCC
  AGCGGGGCACGCGGGGCAGAGACTTCATCTGGCCACGTCCACACTTCACCCACCAGCGAA
  ACGCCACATCGCGAACCCCCGCCCGCGCGACGGGGAAAGGGGAGGGAAAGGCGGGGGGAC
  GGGCGTTCAGGGCGGACCGATGCGCCACCACGGGCGACGACCGGCCGCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_152649
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152649.1, NP_689862.1
• RefSeq Size: 2496 bp
• RefSeq ORF: 1416 bp
• Locus ID: 197259
• UniProt ID: Q8NB16
• Cytogenetics: 16q23.1
• Domains: pkinase
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1, also known as MLKLshRNA-A) represents the longer transcript and encodes the longer isoform (1).