EMP2 (NM_001424) Human Untagged Clone

CAT#: SC108082

EMP2 (untagged)-Human epithelial membrane protein 2 (EMP2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EMP2
• Synonyms: XMP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108082 sequence for NM_001424 edited (data generated by NextGen Sequencing)
  ATGTTGGTGCTTCTTGCTTTCATCATCGCCTTCCACATCACCTCTGCAGCCTTGCTGTTC
  ATTGCCACCGTCGACAATGCCTGGTGGGTAGGAGATGAGTTTTTTGCAGATGTCTGGAGA
  ATATGTACCAACAACACGAATTGCACAGTCATCAATGACAGCTTTCAAGAGTACTCCACG
  CTGCAGGCGGTCCAGGCCACCATGATCCTCTCCACCATTCTCTGCTGCATCGCCTTCTTC
  ATCTTCGTGCTCCAGCTCTTCCGCCTGAAGCAGGGAGAGAGGTTTGTCCTAACCTCCATC
  ATCCAGCTAATGTCATGTCTGTGTGTCATGATTGCGGCCTCCATTTATACAGACAGGCGT
  GAAGACATTCACGACAAAAACGCGAAATTCTATCCCGTGACCAGAGAAGGCAGCTACGGC
  TACTCCTACATCCTGGCGTGGGTGGCCTTCGCCTGCACCTTCATCAGCGGCATGATGTAC
  CTGATACTGAGGAAGCGCAAATAG
  
  Clone variation with respect to NM_001424.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001424 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCCTCGACTCGCC
  CTGCCCTGTGAAAAGTTGGTGCTTCTTGCTTTCATCATCGCCTTCCACATCACCTCTGCA
  GCCTTGCTGTTCATTGCCACCGTCGACAATGCCTGGTGGGTAGGAGATGAGTTTTTTGCA
  GATGTCTGGAGAATATGTACCAACAACACGAATTGCACAGTCATCAATGACAGCTTTCAA
  GAGTACTCCACGCTGCAGGCGGTCCAGGCCACCATGATCCTCTCCACCATTCTCTGCTGC
  ATCGCCTTCTTCATCTTCGTGCTCCAGCTCTTCCGCCTGAAGCAGGGAGAGAGGTTTGTC
  CTAACCTCCATCATCCAGCTAATGTCATGTCTGTGTGTCATGATTGCGGCCTCCATTTAT
  ACAGACAGGCGTGAAGACATTCACGACAAAAACGCGAAATTCTATCCCGTGACCAGAGAA
  GGCAGCTACGGCTACTCCTACATCCTGGCGTGGGTGGCCTTCGCCTGCACCTTCATCAGC
  GGCATGATGTACCTGATACTGAGGAAGCGCAAATAGAGTTCCGGAGCTGGGTTGCTTCTG
  CTGCAGTACAGAATCCACATTCAGATAACCATTTTGTATATAATCATTATTTTTTTGAGG
  TTNTTCTAGCAAACGTNATTGTTTCCTTTTAAAGCCAAAAAAAAAAAAANAAANNNANNA
  NAANAAAAAANANAAAAAAAANNAAAANAAAAAAAAAAANNAAAAAAAAAAAAAAANACC
  CTCATTTTAATTGCCGCCGGTCTATAACTTTTTCTTGACAATCCCCGGGTGGATTCCTGG
  GACCCTCCCAGGCCTTCTGCCCCGTGGAAGTGCCCTTCACGGCCCCACCTGGCTAAAAAA
  ATAGTGCTCATTTGCGACAAGGGCTATN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001424 unedited
  TTTTTTTTTTNNNNNTTNNNTTTTTATTTATTTTTTTTTGAAATTCTTTAGGATAACATA
  CGTTTGCTAGNAAAACCTCAAAAATAATGATTTTACAAAAGGTTCATCTGATGTGGATTC
  TGTACTGCAGCAGAAGCAACCCAGCTCCGGAACTCTATTTGCGCTTCCTCAGTATCAGGT
  ACATCATGCCGCTGATGAAGGTGCAGGCGAAGGCCACCCACGCCAGGATGTAGGAGTAGC
  CGTAGCTGCCTTCTCTGGTCACGGGATAGAATTTCGCGTTTTTGTCGTGAATGTCTTCAC
  GCCTGTCTGTATAAATGGAGGCCGCATTCATGACACACAGACATGACATTACCTGGATGA
  TGGAGGTTAGGACAAACCTCTCTCCCTGCTTCAGGCGGAAGAGCTGGAGCACGAAGATGA
  AGAAGGCGATGCAGCAGATAATGGTGGAGAGGATCATGGTGGCCTGGACCGCCTGCAGCG
  TGGAGTACTCTTGAAAGCTGTCATTGATGACTGTGCAATTCGTGTTGATGGTACATATTC
  TCCAGACATCTGCAAAAAACTCATCTCCTACCCACCCAGCATTGTCGACGGTGGCAATGA
  ACAGCAAGGCTTGACAGGTGATGTGGAAGGCGATGATGAAAGCCAGACACCCCAACCTTT
  TCACAGTGCACGGCCAAGCCAGGCGACTTCGTGCCCGAATTTCCCGGCCGCCCTATAGTG
  AGCCCGGATTACAAAAATTCTGGCGGGTCCACTAAACGAAGCTCTGGTTTATTAAACCTT
  CCCACCGTACACGCCCTACCGCCCAATTTGGGTCCACGGGGCCGGGGTATTTACCACATT
  TTTGGAAAGTTCCCGTTGTTTCTTGGGGGCCCAAACAAAACTCCCCTATGCCCGTTAATT
  GGGCGTGGCAGACTTTGAACATCCCCGGCGAGTANACACGCTTTTGTCACGCCCCATCTG
  CCGGACCTCGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001424
• Insert Size: 660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001424.3, NP_001415.1
• RefSeq Size: 1484 bp
• RefSeq ORF: 504 bp
• Locus ID: 2013
• UniProt ID: P54851
• Cytogenetics: 16p13.13
• Domains: PMP22_Claudin
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a tetraspan protein of the PMP22/EMP family. The encoded protein regulates cell membrane composition. It has been associated with various functions including endocytosis, cell signaling, cell proliferation, cell migration, cell adhesion, cell death, cholesterol homeostasis, urinary albumin excretion, and embryo implantation. It is known to negatively regulate caveolin-1, a scaffolding protein which is the main component of the caveolae plasma membrane invaginations found in most cell types. Through activation of PTK2 it positively regulates vascular endothelial growth factor A. It also modulates the function of specific integrin isomers in the plasma membrane. Up-regulation of this gene has been linked to cancer progression in multiple different tissues. Mutations in this gene have been associated with nephrotic syndrome type 10 (NPHS10). [provided by RefSeq, Mar 2015]