CLC7 (CLCN7) (NM_001287) Human Untagged Clone

CAT#: SC108053

CLCN7 (untagged)-Human chloride channel 7 (CLCN7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLC7
• Synonyms: CLC-7; CLC7; HOD; OPTA2; OPTB4; PPP1R63
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108053 sequence for NM_001287 edited (data generated by NextGen Sequencing)
  ATGGCCAACGTCTCTAAGAAGGTGTCCTGGTCCGGCCGGGACCGGGACGACGAGGAGGCG
  GCGCCGCTGCTGCGGAGGACGGCGCGGCCCGGCGGGGGGACGCCGCTGCTGAACGGGGCT
  GGGCCTGGGGCTGCGCGCCAGTCACCACGTTCTGCGCTTTTCCGAGTCGGACATATGAGC
  AGCGTGGAGCTGGATGATGAACTTTTGGACCCGGATATGGACCCTCCACATCCCTTCCCC
  AAGGAGATCCCACACAACGAGAAGCTCCTGTCCCTCAAGTATGAGAGCTTGGACTATGAC
  AACAGTGAGAACCAGCTGTTCCTGGAGGAGGAGCGGCGGATCAATCACACGGCCTTCCGG
  ACGGTGGAGATCAAGCGCTGGGTCATCTGCGCCCTCATTGGGATCCTCACGGGCCTCGTG
  GCCTGCTTCATTGACATCGTGGTGGAAAACCTGGCTGGCCTCAAGTACAGGGTCATCAAG
  GGCAATATCGACAAGTTCACAGAGAAGGGCGGACTGTCCTTCTCCCTGTTGCTGTGGGCC
  ACGCTGAACGCCGCCTTCGTGCTCGTGGGCTCTGTGATTGTGGCTTTCATAGAGCCGGTG
  GCTGCTGGCAGCGGAATCCCCCAGATCAAGTGCTTCCTCAACGGGGTGAAGATCCCCCAC
  GTGGTGCGGCTCAAGACGTTGGTGATCAAAGTGTCCGGTGTGATCCTGTCCGTGGTCGGG
  GGCCTGGCCGTGGGAAAGGAAGGGCCGATGATCCACTCAGGTTCAGTGATTGCCGCCGGG
  ATCTCTCAGGGAAGGTCAACGTCACTGAAACGAGATTTCAAGATCTTCGAGTACTTCCGC
  AGAGACACAGAGAAGCGGGACTTCGTCTCCGCAGGGGCTGCGGCCGGAGTGTCAGCGGCG
  TTTGGAGCCCCCGTGGGTGGGGTCCTGTTCAGCTTGGAGGAGGGTGCGTCCTTCTGGAAC
  CAGTTCCTGACCTGGAGGATCTTCTTTGCTTCCATGATCTCCACGTTCACCCTGAATTTT
  GTTCTGAGCATTTACCACGGGAACATGTGGGACCTGTCCAGCCCAGGCCTCATCAACTTC
  GGAAGGTTTGACTCGGAGAAAATGGCCTACACGATCCACGAGATCCCGGTCTTCATCGCC
  ATGGGCGTGGTGGGCGGTGTGCTTGGAGCAGTGTTCAATGCCTTGAACTACTGGCTGACC
  ATGTTTCGAATCAGGTACATCCACCGGCCCTGCCTGCAGGTGATTGAGGCCGTGCTGGTG
  GCCGCCGTCACGGCCACAGTTGCCTTCGTGCTGATCTACTCGTCGCGGGATTGCCAGCCC
  CTGCAGGGGGGCTCCATGTCCTACCCGCTGCAGCTCTTTTGTGCAGATGGCGAGTACAAC
  TCCATGGCTGCGGCCTTCTTCAACACCCCGGAGAAGAGCGTGGTGAGCCTCTTCCACGAC
  CCGCCAGGCTCCTACAACCCCCTGACCCTCGGCCTGTTCACGCTGGTCTACTTCTTCCTG
  GCCTGCTGGACCTACGGGCTCACGGTGTCTGCCGGGGTCTTCATCCCGTCCCTGCTCATC
  GGGGCTGCCTGGGGCCGGCTCTTTGGGATCTCCCTGTCCTACCTCACGGGGGCGGCGATC
  TGGGCGGACCCCGGCAAATACGCCCTGATGGGAGCTGCTGCCCAGCTGGGCGGGATTGTG
  CGGATGACACTGAGCCTGACCGTCATCATGATGGAGGCCACCAGCAACGTGACCTACGGC
  TTCCCCATCATGCTGGTGCTCATGACCGCCAAGATCGTGGGCGACGTCTTCATTGAGGGC
  CTGTACGACATGCACATTCAGCTGCAGAGTGTGCCCTTCCTGCACTGGGAGGCCCCGGTC
  ACCTCACACTCACTCACTGCCAGGGAGGTGATGAGCACACCAGTGACCTGCCTGAGGCGG
  CGTGAGAAGGTCGGCGTCATTGTGGACGTGCTGAGCGACACGGCGTCCAATCACAACGGC
  TTCCCCGTGGTGGAGCATGCCGATGACACCCAGCCTGCCCGGCTCCAGGGCCTGATCCTG
  CGCTCCCAGCTCATCGTTCTCCTAAAGCACAAGGTGTTTGTGGAGCGGTCCAACCTGGGC
  CTGGTACAGCGGCGCCTGAGGCTGAAGGACTTCCGAGACGCCTACCCGCGCTTCCCACCC
  ATCCAGTCCATCCACGTGTCCCAGGACGAGCGGGAGTGCACCATGGACCTCTCCGAGTTC
  ATGAACCCCTCCCCCTACACGGTGCCCCAGGAGGCGTCGCTCCCACGGGTGTTCAAGCTG
  TTCCGGGCCCTGGGCCTGCGGCACCTGGTGGTGGTGGACAACCGCAATCAGGTTGTCGGG
  TTGGTGACCAGGAAGGACCTCGCCAGGTACCGCCTGGGAAAGAGAGGCTTGGAGGAGCTC
  TCGCTGGCCCAGACGTGA
  
  Clone variation with respect to NM_001287.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001287 unedited
  NNNNNNNNNNGGGGAAANNNNNNNNNNNNNNNANAAATTTTTCCCCGCCCGTTGCCGCAA
  AGGGCGGTAGGCGTGTACGGTGGGNAGTCTATATAAGCAGNACTCATTTAGGTGACACTA
  TAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCCGCGGCG
  GGCCATGGCCAACGTCTCTAAGAAGGTGTCCTGGTCCGGCCGGGACCGGGACGACGAGGA
  GGCGGCGCCGCTGCTGCGGAGGACGGCGCGGCCCGGCGGGGGGACGCCGCTGCTGAACGG
  GGCTGGGCCTGGGGCTGCGCGCCAGTCACCACGTTCTGCGCTTTTCCGAGTCGGACATAT
  GAGCAGCGTGGAGCTGGATGATGAACTTTTGGACCCGGATATGGACCCTCCACATCCCTT
  CCCCAAGGAGATCCCACACAACGAGAAGCTCCTGTCCCTCAAGTATGAGAGCTTGGACTA
  TGACAACAGTGAGAACCAGCTGTTCCTGGAGGAGGAGCGGCGGATCAATCACACGGCCTT
  CCGGACGGTGGAGATCAAGCGCTGGGTCATCTGCGCCCTCATTGGGATCCTCACGGGCCT
  CGTGGCCTGCTTCATTGACATCGTGGTGGAAAACCTGGCTGGCCTCAAGTACAGGGTCAT
  CAAGGGCAATATCGACAAGTTCACAGAGAAGGGCGGACTGTCCTTCTCCCTGTTGCTGTG
  GGCCACGCTGAACGCCGCCTTCGTGCTCGTGGGCTCTGTGATTGTGGCTTTCATAGAGCC
  GGTGGCTGCTGGCAGCGGAATCCCCCAGATCAAGTGCTTCCTCAACGGNGTGAAGATCCC
  CCACGTGGTGCGGCTCAAGACGTTTGTGATCAAAGTGTCCGTTGTGATCCTGTCCGTGGT
  CGGGGCCCCTGGCGTGGAAAAAGAAAGGCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001287 unedited
  ATACTTATGTACGCGGCCGCATTCTAAGATCGGTTTTTTTTTTTTTTTTTTGTGAATCCT
  AACCGCTGATTTTATTTAACCGAATATATCCAAAATTGATCACTTCCAGGTCCTCAGTAC
  ACAACCACCACGAGGGAAACCCAGACCTAGGTTTCCGGCGCCGACTCTGAGGCCCGGGAG
  TTTTCTTCTTGCGTCACCCCAGCGTGGGCACCCCGGCCCGCCCCCCTCCACCTGGAGCGC
  CTCTGCCTTGCCCCCAGACCCACCAGCCAGGAGCCAGAGGAGGGAGGCTGAGGGGCGGGG
  ACGAGGAAGAGGATGTGGCTGGTGCCCTGGGTGGCCGCAGCCGCTGTCTTTCACGCCTGT
  CTCACTCCACACGGTGCCCCGACCCCAGGGTCCCGAAAGTCCCAAGCTGTTGGACGTGGT
  CTGGGTGGGGTGGGAGGGACGACGCTGGCAAAGGCGGGCTGAGAGGATTTCTTTATTTCG
  CGTTCAGACCCAACACACTCGGACACCTTGGGGCCCTGTGAGGGCTAAGCAGGGTGGTGG
  CGTCAGCTCCCGGGGAGGCCCCACTCCCTGGGGAGGAAATACACGGCAGGGNGGCCGCAC
  CCAGCCCCCCACGGATGGACCCGTGTTGCTCTAACAAGGACACTGAAGTTGCCTCTGCCG
  CCCCGTGAAGGGCCTGTGGCGGGCCCCAGACCCAGCCCAGCCAGGCCAGAAGAGCCTCCA
  AGGGGCCCTCNAGTGGTGAAGTGGGGGGCTTCCCGGCCCCGGTGCCCACCGGCCCCTCTA
  NCTGCCTGTGCTGGGGCCAGTGTTCTCTGAGGCGGCAGGGATTCTGGCCCCCGCTGTACC
  TGGCCTTGGTGGCTGCCAGGGCCAAGTAAGGAAGGTACCCTCGAGACCGGGTTCCCCTGT
  GCCTAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001287
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: A TrueClone.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287.3, NP_001278.1
• RefSeq Size: 3760 bp
• RefSeq ORF: 2418 bp
• Locus ID: 1186
• UniProt ID: P51798
• Cytogenetics: 16p13.3
• Domains: CBS, voltage_CLC
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Gene Summary: The product of this gene belongs to the CLC chloride channel family of proteins. Chloride channels play important roles in the plasma membrane and in intracellular organelles. This gene encodes chloride channel 7. Defects in this gene are the cause of osteopetrosis autosomal recessive type 4 (OPTB4), also called infantile malignant osteopetrosis type 2 as well as the cause of autosomal dominant osteopetrosis type 2 (OPTA2), also called autosomal dominant Albers-Schonberg disease or marble disease autosoml dominant. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. OPTA2 is the most common form of osteopetrosis, occurring in adolescence or adulthood. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.