hnRNP K (HNRNPK) (NM_031262) Human Untagged Clone

CAT#: SC107869

HNRNPK (untagged)-Human heterogeneous nuclear ribonucleoprotein K (HNRNPK), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: hnRNP K
• Synonyms: AUKS; CSBP; HNRPK; TUNP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_031262, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAACTGAACAGCCAGAAGAAACCTTCCCTAACACTGAAACCAATGGTGAATTTGGTAAACGCCCTG
  CAGAAGATATGGAAGAGGAACAAGCATTTAAAAGATCTAGAAACACTGATGAGATGGTTGAATTACGCAT
  TCTGCTTCAGAGCAAGAATGCTGGGGCAGTGATTGGAAAAGGAGGCAAGAATATTAAGGCTCTCCGTACA
  GACTACAATGCCAGTGTTTCAGTCCCAGACAGCAGTGGCCCCGAGCGCATATTGAGTATCAGTGCTGATA
  TTGAAACAATTGGAGAAATTCTGAAGAAAATCATCCCTACCTTGGAAGAGGGCCTGCAGTTGCCATCACC
  CACTGCAACCAGCCAGCTCCCGCTCGAATCTGATGCTGTGGAATGCTTAAATTACCAACACTATAAAGGA
  AGTGACTTTGACTGCGAGTTGAGGCTGTTGATTCATCAGAGTCTAGCAGGAGGAATTATTGGGGTCAAAG
  GTGCTAAAATCAAAGAACTTCGAGAGAACACTCAAACCACCATCAAGCTTTTCCAGGAATGCTGTCCTCA
  TTCCACTGACAGAGTTGTTCTTATTGGAGGAAAACCCGATAGGGTTGTAGAGTGCATAAAGATCATCCTT
  GATCTTATATCTGAGTCTCCCATCAAAGGACGTGCACAGCCTTATGATCCCAATTTTTACGATGAAACCT
  ATGATTATGGTGGTTTTACAATGATGTTTGATGACCGTCGCGGACGCCCAGTGGGATTTCCCATGCGGGG
  AAGAGGTGGTTTTGACAGAATGCCTCCTGGTCGGGGTGGGCGTCCCATGCCTCCATCTAGAAGAGATTAT
  GATGATATGAGCCCTCGTCGAGGACCACCTCCCCCTCCTCCCGGACGAGGCGGCCGGGGTGGTAGCAGAG
  CTCGGAATCTTCCTCTTCCTCCACCACCACCACCTAGAGGGGGAGACCTCATGGCCTATGACAGAAGAGG
  GAGACCTGGAGACCGTTACGACGGCATGGTTGGTTTCAGTGCTGATGAAACTTGGGACTCTGCAATAGAT
  ACATGGAGCCCATCAGAATGGCAGATGGCTTATGAACCACAGGGTGGCTCCGGATATGATTATTCCTATG
  CAGGGGGTCGTGGCTCATATGGTGATCTTGGTGGACCTATTATTACTACACAAGTAACTATTCCCAAAGA
  TTTGGCTGGATCTATTATTGGCAAAGGTGGTCAGCGGATTAAACAAATCCGTCATGAGTCGGGAGCTTCG
  ATCAAAATTGATGAGCCTTTAGAAGGATCCGAAGATCGGATCATTACCATTACAGGAACACAGGACCAGA
  TACAGAATGCACAGTATTTGCTGCAGAACAGTGTGAAGCAGTATTCTGGAAAGTTTTTCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031262 unedited
  GGAATTTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGAGGCGGCGCG
  GCAGCGGAGGGACGGCAGTCTCGCGCGGCTACTGCAGCACTGGGGTGTCAGTTGTTGGTC
  CGACCCAGAACGCTTCAGTTCTGCTCTGCAAGGATATATAATAACTGATTGGTGTGCCCG
  TTTAATAAAAGAATATGGAAACTGAACAGCCAGAAGAAACCTTCCCTAACACTGAAACCA
  ATGGTGAATTTGGTAAACGCCCTGCAGAAGATATGGAAGAGGAACAAGCATTTAAAAGAT
  CTAGAAACACTGATGAGATGGTTGAATTACGCATTCTGCTTCAGAGCAAGAATGCTGGGG
  CAGTGATTGGAAAAGGAGGCAAGAATATTAAGGCTCTCCGTACAGACTACAATGCCAGTG
  TTTCAGTCCCAGACAGCAGTGGCCCCGAGCGCATATTGAGTATCAGTGCTGATATTGAAA
  CAATTGGAGAAATTCTGAAGAAAATCATCCCTACCTTGGAAGAGGGCCTGCAGTTGCCAT
  CACCCACTGCAACCAGCCAGCTCCCGCTCGAATCTGATGCTGTGGAATGCTTAAATTACC
  AACACTATAAAGGAAGTGACTTTGACTGCGAGTTGAGGCTGTTGATTCATCAGAGTCTAG
  CAGGAGGAATTATTGNNGGTCAAAGTGCTAAAATCAAAGAACTTCGAGAGAACACTCAAA
  CCACCATCAAGCTTTTNNCAGAATGCTGTCCTCATTCCCACTGACAGANTGTTCTTATTG
  GAGGAAAAACCCGATAGGNTGGTANAGTGCATAAAGATCTNCCTTGATCTTATATCTGAG
  TCTCCATCAAAAGACGTGCACAGCCTTATGATCCCCATTTTTACGATGAACTATGAAATA
  TGGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031262 unedited
  GAGTCGAGTTTTTTTTTTTTTTTTTTTAGCATCATGGGTGTTTTATTTATTTGGAAGTGA
  ATTTTAACTATCAATACAAATGCCAAGATACTACACAAAACATCCACAGGAACTTTTTTC
  ATCTTTTTTTTTTTTGAATTGTACACAAACATTTCCTACATAATCCAACATACAACAGAG
  AAATGGTACATCTTTTTCTTTCAATTTGCATACAATGGAAAAACAAGTATATATATATTT
  TACAAAGTTTAACTAATAAGACACTAGAGCAAATTGACAGTTTAAGTCTATAGGTGAGAA
  ATTATCTAATAAAAATAATCAATTTTTTCAGCATTAGTCACTTCCATAACCAAGTGTTAT
  TCTGATTAATAAAACTTGCTGCCAATCAGAATTCTGGTATATACTCATACCATTACTCAA
  CTTGTAGTACTGCTCCCCACCTTAGTTCTTCACAACTAACATAGAAAATTGTTGAAAAGT
  AGGGGCAAGCATTTGCAAAAACAAACAAAAAATCCCCAGCTTATTATAAGCATGAATATG
  TATGATGGAATTTCTTCCCAGCAATAGACTTCCCCAACCATCAGAAATCACCAGAACTAA
  AGTTGCCAAGTTATTTTGCCTATGTCCAACAAGAGATGCACTTATATGTCCAACAGAGGA
  GCTGAAAATAAACTTAGTGTTTAGTTGGGGGGTGGGTTGGGAAATNCAGCAACATTTTAA
  ATGACTGTCTTAAAAAAAAATGACCCCCAAAATAGGTTCACTAAATTTATTTAAAAATCA
  TAAAACGTTTCTTACAAAGAAGCATACATTTCTGCACACTGCTCTGAACAGATGCAGGAA
  CATGGGGCCATTTGTACTTTTCCTCCTGTCCCACCCCCAATGTTCAAGTGACCCAAAGCA
  AGGGGTTAAATAATTACCTGGGGGGGATTTTTTAAACACCACCACTACCGAAAATAAATC
  CCCTCCTCTGCCGTGTTNAAAATTCAATGTAGTTTTTGCCGCCCTCCCCCCCCACCTGTT
  GTTAGAACAAACTTCCTTGTGACACCAAGTTTCTCACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031262
• Insert Size: 2840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031262.1, NP_112552.1
• RefSeq Size: 2770 bp
• RefSeq ORF: 1392 bp
• Locus ID: 3190
• UniProt ID: P61978
• Cytogenetics: 9q21.32
• Domains: KH
• Protein Pathways: Spliceosome
• Gene Summary: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) uses an alternate acceptor splice site at the last coding exon compared to transcript variant 1. This results in a frame-shift and an isoform (b) with a distinct C-terminus compared to isoform a.