Phospholipase C gamma 1 (PLCG1) (NM_182811) Human Untagged Clone

CAT#: SC107651

PLCG1 (untagged)-Human phospholipase C, gamma 1 (PLCG1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Phospholipase C gamma 1
• Synonyms: NCKAP3; PLC-II; PLC1; PLC148; PLCgamma1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107651 sequence for NM_182811 edited (data generated by NextGen Sequencing)
  ATGGCGGGCGCCGCGTCCCCTTGCGCCAACGGCTGCGGGCCCGGCGCGCCCTCGGACGCC
  GAGGTGCTGCACCTCTGCCGCAGCCTCGAGGTGGGCACCGTCATGACTTTGTTCTACTCC
  AAGAAGTCGCAGCGACCCGAGCGGAAGACCTTCCAGGTCAAGCTGGAGACGCGCCAGATC
  ACGTGGAGCCGGGGCGCCGACAAGATCGAGGGGGCCATTGACATTCGTGAAATTAAGGAG
  ATCCGCCCAGGGAAGACCTCACGGGACTTTGATCGCTATCAAGAGGACCCAGCTTTCCGG
  CCGGACCAGTCACATTGCTTTGTCATTCTCTATGGAATGGAATTTCGCCTGAAAACGCTG
  AGCCTGCAAGCCACATCTGAGGATGAAGTGAACATGTGGATCAAGGGCTTAACTTGGCTG
  ATGGAGGATACATTGCAGGCACCCACACCCCTGCAGATTGAGAGGTGGCTCCGGAAGCAG
  TTTTACTCAGTGGATCGGAATCGTGAGGATCGTATATCAGCCAAGGACCTGAAGAACATG
  CTGTCCCAGGTCAACTACCGGGTCCCCAACATGCGCTTCCTCCGAGAGCGGCTGACGGAC
  CTGGAGCAGCGCAGCGGGGACATCACCTACGGGCAGTTTGCTCAGCTGTACCGCAGCCTC
  ATGTACAGCGCCCAGAAGACGATGGACCTCCCCTTCTTGGAAGCCAGTACTCTGAGGGCT
  GGGGAGCGGCCGGAGCTTTGCCGAGTGTCCCTTCCTGAGTTCCAGCAGTTCCTTCTTGAC
  TACCAGGGGGAGCTGTGGGCTGTTGATCGCCTCCAGGTGCAGGAGTTCATGCTCAGCTTC
  CTCCGAGACCCCTTACGAGAGATCGAGGAGCCATACTTCTTCCTGGATGAGTTTGTCACC
  TTCCTGTTCTCCAAAGAGAACAGTGTGTGGAACTCGCAGCTGGATGCAGTATGCCCGGAC
  ACCATGAACAACCCTCTTTCCCACTACTGGATCTCCTCCTCGCACAACACGTACCTGACC
  GGGGACCAGTTCTCCAGTGAGTCCTCCTTGGAAGCCTATGCTCGCTGCCTGCGGATGGGC
  TGTCGCTGCATTGAGTTGGACTGCTGGGACGGCCCGGATGGGATGCCAGTTATTTACCAT
  GGGCACACCCTTACCACCAAGATCAAGTTCTCAGATGTCCTGCACACCATCAAGGAGCAT
  GCCTTTGTGGCCTCAGAGTACCCAGTCATCCTGTCCATTGAGGACCACTGCAGCATTGCC
  CAGCAGAGAAACATGGCCCAATACTTCAAGAAGGTGCTGGGGGACACACTCCTCACCAAG
  CCCGTGGAGATCTCTGCCGACGGGCTCCCCTCACCCAACCAGCTTAAGAGGAAGATCCTC
  ATCAAGCACAAGAAGCTGGCTGAGGGCAGTGCCTACGAGGAGGTGCCTACATCCATGATG
  TACTCTGAGAACGACATCAGCAACTCTATCAAGAATGGCATCCTCTACCTGGAGGACCCT
  GTGAACCACGAATGGTATCCCCACTACTTTGTTCTGACCAGCAGCAAGATCTACTACTCT
  GAGGAGACCAGCAGTGACCAGGGCAACGAGGATGAGGAGGAGCCCAAGGAGGTCAGCAGC
  AGCACAGAGCTGCACTCCAATGAGAAGTGGTTCCATGGGAAGCTAGGGGCAGGGCGTGAC
  GGGCGTCACATCGCTGAGCGCCTGCTTACTGAGTACTGCATCGAGACCGGAGCCCCTGAC
  GGCTCCTTCCTCGTGCGAGAGAGTGAGACCTTCGTGGGCGACTACACGCTCTCTTTCTGG
  CGGAACGGGAAAGTCCAGCACTGCCGTGTCCACTCCCGGCAAGATGCTGGGACCCCCAAG
  TTCTTCTTGACAGACAACCTCGTCTTTGACTCCCTCTATGACCTCATCACGCACTACCAG
  CAGGTGCCCCTGCGCTGTAATGAGTTTGAGATGCGACTTTCAGAGCCTGTCCCACAGACC
  AACGCCCACGAGAGCAAAGAGTGGTACCACGCGAGCCTGACCAGAGCACAGGCTGAGCAC
  ATGCTAATGCGCGTCCCTCGTGATGGGGCCTTCCTGGTGCGGAAGCGGAATGAACCCAAC
  TCATATGCCATCTCTTTCCGGGCTGAGGGCAAGATCAAGCATTGCCGTGTCCAGCAAGAG
  GGCCAGACAGTGATGCTAGGGAACTCGGAGTTCGACAGCCTTGTTGACCTCATCAGCTAC
  TATGAGAAACACCCGCTATACCGCAAGATGAAGCTGCGCTATCCCATCAACGAGGAGGCA
  CTGGAGAAGATTGGCACAGCTGAGCCTGACTACGGGGCCCTGTATGAGGGACGCAACCCT
  GGCTTCTATGTAGAGGCAAACCCTATGCCAACTTTCAAGTGTGCAGTCAAAGCCCTCTTT
  GACTACAAGGCCCAGAGGGAGGACGAGCTGACCTTCACCAAGAGCGCCATCATCCAGAAT
  GTGGAGAAGCAAGAGGGAGGCTGGTGGCGAGGGGACTACGGAGGGAAGAAGCAGCTGTGG
  TTCCCATCAAACTACGTGGAAGAGATGGTCAACCCCGTGGCCCTGGAGCCGGAGAGGGAG
  CACTTGGACGAGAACAGCCCCCTAGGGGACTTGCTGCGGGGGGTCTTGGATGTGCCGGCT
  TGTCAGATTGCCATCCGTCCTGAGGGCAAGAACAACCGGCTCTTCGTCTTCTCCATCAGC
  ATGGCGTCGGTGGCCCACTGGTCCCTGGATGTTGCTGCCGACTCACAGGAGGAGCTGCAG
  GACTGGGTGAAAAAGATCCGTGAAGTGGCCCAGACAGCAGACGCCAGGCTCACTGAAGGG
  AAGATAATGGAACGGAGGAAGAAGATTGCCCTGGAGCTCTCTGAACTTGTCGTCTACTGC
  CGGCCTGTTCCCTTTGATGAAGAGAAGATTGGCACAGAACGTGCTTGCTACCGGGACATG
  TCATCCTTCCCGGAAACCAAGGCTGAGAAATACGTGAACAAGGCCAAAGGCAAGAAGTTC
  CTTCAGTACAATCGACTGCAGCTCTCCCGCATCTACCCCAAGGGCCAGCGACTGGATTCC
  TCCAACTACGATCCTTTGCCCATGTGGATCTGTGGCAGTCAGCTTGTGGCCCTCAACTTC
  CAGACCCCTGACAAGCCTATGCAGATGAACCAGGCCCTCTTCATGACGGGCAGGCACTGT
  GGCTACGTGCTGCAGCCAAGCACCATGCGGGATGAGGCCTTCGACCCCTTTGACAAGAGC
  AGCCTCCGCGGGCTGGAGCCATGTGCCATCTCTATTGAGGTGCTGGGGGCCCGACATCTG
  CCAAAGAATGGCCGAGGCATTGTGTGTCCTTTTGTGGAGATTGAGGTGGCTGGAGCTGAG
  TATGACAGCACCAAGCAGAAGACAGAGTTTGTGGTGGACAATGGACTCAACCCTGTATGG
  CCAGCCAAGCCCTTCCACTTCCAGATCAGTAACCCTGAATTTGCCTTTCTGCGCTTCGTG
  GTGTATGAGGAAGACATGTTTAGTGACCAGAATTTCCTGGCTCAGGCTACTTTCCCAGTA
  AAAGGCCTGAAGACAGGATACAGAGCAGTGCCTTTGAAGAACAACTACAGTGAGGACCTG
  GAGTTGGCCTCCCTGCTGATCAAGATTGACATTTTCCCTGCCAAGGAGAATGGTGACCTC
  AGTCCCTTCAGTGGTACGTCCCTGCGGGAGCGGGGCTCAGATGCCTCAGGCCAGCTGTTT
  CATGGCCGAGCCCGGGAAGGCTCCTTTGAATCCCGCTACCAGCAGCCGTTTGAGGACTTC
  CGCATCTCCCAGGAGCATCTCGCAGACCATTTTGACAGTCGAGAACGAAGGGCCCCAAGA
  AGGACTCGGGTCAATGGAGACAACCGCCTCTAG
  
  Clone variation with respect to NM_182811.1
  1828 a=>g;2438 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_182811 unedited
  CTATAGGGCGGCCGCGAATTCGCACGAGGGCGCGCGGAGGACCCGCCGCCGCCGGTGTGA
  GGCGGCCCGTCCTGGCTCCCTTGTCCGGGAAGCCCGCCCAGGAGCCGCCGCCGGGTCCCG
  CTCGTCTGCCGCCTCAGCCTCAGCCCCAACCTCAGCCGCCGCCGTTGCGCTTGCTCCCGG
  GCGGTCCTGGCCTGTGCCGCCGCCGCCCCCAGCGTCGGAGCCATGGCGGGCGCCGCGTCC
  CCTTGCGCCAACGGCTGCGGGCCCGGCGCGCCCTCGGACGCCGAGGTGCTGCACCTCTGC
  CGCAGCCTCGAGGTGGGCACCGTCATGACTTTGTTCTACTCCAAGAAGTCGCAGCGACCC
  GAGCGGAAGACCTTCCAGGTCAAGCTGGAGACGCGCCAGATCACGTGGAGCCGGGGCGCC
  GACAAGATCGAGGGGGCCATTGACATTCGTGAAATTAAGGAGATCCGCCCAGGGAAGACC
  TCACGGGACTTTGATCGCTATCAAGAGGACCCAGCTTTCCGGCCGGACCAGTCACATTGC
  TTTGTCATTCTCTATGGAATGGAATTTCGCCTGAAAACGCTGAGCCTGCAAGCCACATCT
  GAGGATGAAGTGAACATGTGGATCAAGGGCTTAACTTGGCTGATGGATGATACATTGCAG
  GCACCCACACCCCTGCAGATTGAGAGGTGGCTCCGGAAGCAGTTTTACTCAGTGGATCGG
  AATCGTGAGGATCGTATATCAGCCAAGGACCTGAAGAAACATGCTGTCCANGTCAACTAC
  CGGGTCCCCAACATGCGCTTTCTCCGAGAGCGGCTGACGGACCTGGAGCAGCGCAGCGGG
  GACATCACCTACGGGCAGTTTGCTCAGCTGTACGGCAGCCTCATGTACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_182811 unedited
  TATGTACCGTCGGCCGCAATCCANAGTCGGTTTTTTTTTTTTTTTTTTGAATGAAAATGA
  TTTTTAAATTTGCTTGAGAGGGGGCACGTTTGTCCACTAAGAAAAAACAATCAAAACATT
  TGCAGTGAGGCAAAGGCAATGTCTTACAGAGCCCAAGAGAGGTGAGCCTCAGGTCTACAA
  AGCTGCCTTAAGAAAGGCAGAGCTGTTTGTTTCCCTGATGTGGGCCAGAAAGACTTTTGG
  GGGGGGATATAGATACGTATTGATGAAAACTAAAAGGGTCAACGGGGGGGGGGACTCCTA
  GGACGTCCTAACCAACGTGGATGGGCCCTAACACAAGGGCCTACACAGTCCTCTTCCTTC
  TCTGATAGGAAACCTTTCTCTCCAGGCCCTAAAAACCTACAGAGGAAACATGGATCATGA
  GGGCCCCGGTGAATTCCCACCCCCCACCCTCTTGTGTTTGGGAAAAAACCACCCTCCTCC
  CTTTTAACTGGGACTGTGAAGCTGGTAAAGAAAGCACACAAGAACCTGAGCACTATCCCT
  GTTTGGTACTGGCTATCATAAATTTATGTGATCCCCGGGACCGGGCCTAGTCTAAAATTT
  TTTTGTGGCGGGGTTTTTTGTTCTTCCCCCCCACCCCCCCATGTTAAACAACTTCCTAAA
  ATAAAGGGCCTTTCCCTGTTTTTTTATGGGGCCGAGTATTTATCTTTGCTGTCCTACGAG
  CCATCTAATAGAACACCGCACGGGTGTCCGAACTCGGAGGCAACATCTTTTTAATATAAA
  GGTTTATTTTCGTGACCTGATAAGGGGGTCCGGTGTGTTTTTCTGTTTTTTCACACAACA
  CGGGCTGGCGGCAGCGAAACCAACACAGAAGGGACGTTGGGTTTTACTCTCCCCCCCCAG
  CCGGGGTGTGGAGAGTGAGGGGGAACACTCCCGTTCCCCCCGAAGGGGGAACAGCACGCG
  CGTCGTTTTCCGAGATATACACAATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_182811
• Insert Size: 6300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182811.1, NP_877963.1
• RefSeq Size: 5202 bp
• RefSeq ORF: 3873 bp
• Locus ID: 5335
• UniProt ID: P19174
• Cytogenetics: 20q12
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glioma, Inositol phosphate metabolism, Leukocyte transendothelial migration, Metabolic pathways, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, T cell receptor signaling pathway, VEGF signaling pathway, Vibrio cholerae infection
• Gene Summary: The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.