VPS50 (NM_024553) Human Untagged Clone

CAT#: SC107550

CCDC132 (untagged)-Human coiled-coil domain containing 132 (CCDC132), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VPS50
• Synonyms: CCDC132; VPS54L
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_024553 edited
  CAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACC
  GTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGGCTT
  CCTAGTGTCAGGGCCAGCTGTGTAGTGGCTCGGTGTGATTTGTTAGCTCTTTGAGGCAGG
  GTACCCTCCTCAGGATTTCGATATGCAAAAAATCAAATCTCTCATGACCCGACAGGGTCT
  GAAAAGCCCTCAAGAAAGCCTCAGTGATCTTGGTGCCATAGAGAGTCTCCGGGTCCCTGG
  AAAGGAAGAATTCAGGGAACTTCGAGAACAGCCAAGTGACCCTCAAGCTGAACAAGAGCT
  TATTAATAGTATTGAACAAGTATATTTTTCTGTGGATTCATTTGATATTGTTAAATATGA
  GCTGGAGAAGCTTCCACCTGTTCTCAATTTGCAAGAATTAGAGGCGTATAGAGACAAATT
  GAAACAACAGCAAGCTGCAGTATCTAAAAAAGTGGCAGATTTAATCCTTGAAAAACAGCC
  TGCTTATGTAAAGGAACTTGAAAGAGTTACCTCATTGCAGACAGGTCTTCAATTAGCTGC
  TGTTATCTGTACAAATGGGAGAAGACACTTGAATATTGCAAAGGAAGGTTTTACTCAAGC
  TAGTTTAGGCCTTCTTGCAAATCAAAGGAAACGTCAGTTGCTGATTGGACTTCTGAAATC
  TCTGAGAACTATAAAAACATTGCAAAGAACAGATGTACGGTTAAGTGAAATGCTGGAGGA
  GGAAGATTATCCAGGAGCTATTCAGTTGTGCCTTGAATGTCAAAAAGCTGCCAGCACTTT
  TAAACATTACAGTTGTATAAGTGAACTGAATTCAAAGCTGCAAGATACTTTGGAACAGAT
  TGAGGAACAGCTGGACGTAGCTCTTTCCAAAATCTGCAAGAATTTTGACATTAACCATTA
  TACCAAGGTTCAACAAGCTTATCGACTTCTTGGAAAAACACAGACAGCAATGGATCAACT
  TCATATGCACTTCACCCAAGCCATTCACAACACCGTGTTTCAAGTTGTTCTTGGTTATGT
  GGAACTATGTGCAGGAAACACAGACACAAAATTCCAAAAGCTGCAATATAAGGATCTCTG
  TACACATGTTACACCAGACAGCTATATTCCATGCCTTGCAGACCTGTGCAAAGCACTATG
  GGAAGTTATGCTCAGCTATTATAGGACTATGGAATGGCATGAAAAGCATGACAATGAGGA
  TACTGCTTCAGCTTCTGAAGGGAGTAATATGATAGGTACTGAAGAAACTAATTTTGATCG
  TGGCTACATAAAAAAGAAATTAGAACATGGACTTACACGAATATGGCAGGATGTTCAGCT
  AAAAGTAAAAACCTACTTGCTTGGAACTGATTTGTCTATATTCAAATATGATGATTTCAT
  CTTTGTTTTGGATATAATCAGCAGGTTGATGCAAGTTGGAGAAGAATTTTGTGGTAGCAA
  GTCTGAAGTTTTACAGGAATCTATTAGAAAACAAAGTGTCAATTATTTCAAGAATTACCA
  TAGAACACGGCTCGATGAACTGAGAATGTTCTTAGAGAATGAGACTTGGGAACTTTGTCC
  TGTTAAGTCAAATTTCAGCATCTTGCAACTTCATGAATTTAAATTCATGGAACAGTCTCG
  CTCCCCATCAGTTTCACCTAGTAAACAGCCAGTCTCAACTTCTTCAAAAACAGTGACCTT
  GTTTGAGCAGTACTGTAGTGGTGGGAATCCATTTGAAATTCAGGCCAACCACAAAGATGA
  AGAAACAGAAGATGTCTTAGCTTCTAATGGGTATGAATCTGATGAACAAGAAAAGAGTGC
  CTATCAAGAGTATGACAGTGACAGTGATGTTCCTGAGGAACTCAAACGAGACTATGTGGA
  TGAGCAGACAGGAGATGGTCCTGTGAAAAGTGTTTCTCGGGAAACTCTAAAAAGCAGGAA
  GAAATCAGATTACAGTCTAAATAAAGTGAATGCACCTATCTTAACAAATACAACATTGAA
  CGTCATAAGACTTGTTGGAAAATATATGCAGATGATGAACATTCTTAAGCCAATTGCCTT
  TGATGTTATTCATTTCATGTCTCAACTATTTGATTATTACTTGTATGCAATATATACCTT
  TTTTGGTCGGAATGATTCATTGGAATCAACTGGACTCGGCCTTAGTAGTAGTAGACTAAG
  AACAACTCTAAACAGAATACAAGAAAGCCTTATTGATCTAGAAGTTTCAGCTGATCCTAC
  TGCCACACTCACAGCAGCAGAAGAAAGAAAGGAGAAGGTGCCAAGTCCACACCTCAGTCA
  CCTAGTGGTTTTGACATCTGGGGATACGTTGTATGGGTTGGCAGAAAGAGTGGTAGCCAC
  GGAATCCTTGGTATTCTTGGCTGAACAGTTTGAGTTCCTTCAGCCACATCTGGATGCTGT
  GATGCCTGCAGTCAAAAAGCCCTTTCTTCAGCAGTTCTATTCTCAGACAGTCTCAACCGC
  CAGTGAACTACGGAAACCAATTTACTGGATTGTAGCTGGTAAAGCCCTTGATTATGAACA
  GATGCTGCTTCTCATGGCTAATGTGAAATGGGATGTAAAAGAAATTATGTCACAGCACAA
  CATATATGTAGATGCACTATTAAAGGAATTTGAGCAGTTTAACAGGAGGCTAAATGAAGT
  TTCTAAGAGAGTTCGCATACCCTTGCCTGTGTCTAATATACTTTGGGAACATTGTATACG
  ATTGGCTAATCGAACTATTGTAGAAGGATATGCCAATGTCAAGAAATGCAGTAATGAGGG
  TCGTGCCCTGATGCAACTGGATTTTCAACAGTTTTTAATGAAACTTGAAAAACTAACAGA
  TATTAGACCCATTCCTGATAAAGAATTTGTAGAAACTTATATTAAAGCTTATTACCTAAC
  TGAGAATGACATGGAACGGTGGATCAAAGAGCACAGGGAATATTCAACGAAGCAGCTGAC
  CAATCTGGTGAATGTTTGCCTGGGATCCCATATCAATAAGAAAGCAAGACAAAAACTTCT
  AGCAGCTATAGATGATATAGACAGACCTAAAAGATAATGAACACAGCTCTCTTTCCTCAA
  TGGCATTGATCCTCACTCAACATATATGACCTGAAAGCCAGTTTTTTTATGCACTTCTGA
  CAACTATCTGCTAAGAAAACTTTGTGCATGTTTTTTTGACTGGAAAGTGGAAAATATTGA
  AATGTGTGTGGTGTTCTCATGACTTTTATATGCTGTGGTCTCTTCAACTTTTGGTCTCAT
  TTGTTGTAATCTGAAATGATGTTGCCGCCTTGTCATAACAATGGTTATGTGACTACAGTT
  ATACATTTTACAGAAGAATGTACCATAAGTATATAATTAGAAGAACAGTGGCTTAATATA
  TGTATGGGAAGTTTATGGAAAATGAAGTTGGCACTTTTCTACCCTCTGAGCTTGGTTCTT
  AATAAGCATAATGTGAGGGTGAATATGTAGTATCTCCTAATTATGAGCACTGCATGAGAA
  TTAAAAAACACATGTAAGTAAAATAGTTGAAAAATCAGTATGTTCTCTGTTTTTAAAATG
  TCAAAGTTTATGTCAGGGTTAATTTAGTTATAACAAAGTGATCATAATGGTGAAATTTAA
  TAAATATACTCTAGTATGAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCG
  GTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024553 unedited
  GAGGTGGCTTCCTAGTGTCAGGGCCAGCTGTGTAGTGGCTCGGTGTGATTTGTTAGCTCT
  TTGAGGCAGGGTACCCTCCTCAGGATTTCGATATGCAAAAAATCAAATCTCTCATGACCC
  GACAGGGTCTGAAAAGCCCTCAAGAAAGCCTCAGTGATCTTGGTGCCATAGAGAGTCTCC
  GGGTCCCTGGAAAGGAAGAATTCAGGGAACTTCGAGAACAGCCAAGTGACCCTCAAGCTG
  AACAAGAGCTTATTAATAGTATTGAACAAGTATATTTTTCTGTGGATTCATTTGATATTG
  TTAAATATGAGCTGGAGAAGCTTCCACCTGTTCTCAATTTGCAAGAATTAGAGGCGTATA
  GAGACAAATTGAAACAACAGCAAGCTGCAGTATCTAAAAAAGTGGCAGATTTAATCCTTG
  AAAAACAGCCTGCTTATGTAAAGGAACTTGAAAGAGTTACCTCATTGCAGACAGGTCTTC
  AATTAGCTGCTGTTATCTGTACAAATGGGAGAAGACACTTGAATATTGCAAAGGAAGGTT
  TTACTCAAGCTAGTTTAGGCCTTCTTGCAAATCAAAGGAAACGTCAGTTGCTGATTGGAC
  TTCTGAAATCTCTGAGAACTATAAAAACATTGCAAAGAACAGATGTACGGGTTAAGTGAA
  ATGCTGGAGGAGGAAGATTATCCAGGAGCTATTCAGTTGTGCCTTGAATGTCAAAAAGCT
  GCCAGCACTTTTAAACATTACAGNTGTATAAGTGAACTGAATTCAAAGCTGCAGANACTT
  TGGNACAGATTGAGGAACAGCTGGACGTAACTCTTTCCAAATCTGCAGAATTTTGACATT
  AACCATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024553
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024553.2, NP_078829.1
• RefSeq Size: 1216 bp
• RefSeq ORF: 984 bp
• Locus ID: 55610
• UniProt ID: Q96JG6
• Cytogenetics: 7q21.2-q21.3
• Gene Summary: Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane. Within the EARP complex, required to tether the complex to recycling endosomes. Not involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a.