CPEB4 (NM_030627) Human Untagged Clone

CAT#: SC107390

CPEB4 (untagged)-Human cytoplasmic polyadenylation element binding protein 4 (CPEB4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CPEB4
• Synonyms: CPE-BP4; hCPEB-4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_030627, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGGATTACGGGTTTGGAGTGCTAGTGCAAAGCAATACTGGGAATAAATCTGCTTTTCCAGTCAGAT
  TCCATCCACATCTGCAGCCTCCACACCATCACCAAAATGCCACCCCCAGCCCTGCTGCTTTTATAAATAA
  TAACACAGCTGCCAATGGCAGCAGTGCTGGGTCAGCTTGGCTTTTTCCTGCTCCAGCTACCCATAACATT
  CAGGATGAGATCTTGGGGTCAGAAAAAGCAAAAAGTCAGCAACAGGAACAGCAAGACCCCTTAGAAAAGC
  AGCAGCTTTCCCCAAGTCCAGGTCAGGAAGCTGGAATACTGCCTGAAACAGAGAAGGCAAAATCAGAAGA
  AAATCAAGGGGACAATTCTTCGGAAAATGGCAATGGGAAGGAGAAAATAAGGATCGAATCTCCAGTGTTG
  ACAGGGTTTGATTATCAAGAAGCCACTGGGCTAGGTACTTCAACCCAACCCTTGACATCTAGCGCATCGT
  CTCTTACTGGTTTCAGTAACTGGTCAGCAGCGATAGCGCCTTCCTCCTCTACAATAATCAATGAAGATGC
  AAGTTTCTTTCACCAGGGAGGGGTCCCTGCTGCTTCGGCTAATAACGGTGCTCTGTTGTTTCAAAATTTC
  CCCCATCATGTCAGCCCTGGCTTTGGAGGCAGCTTCTCTCCTCAGATCGGGCCTCTCTCACAGCACCACC
  CACATCACCCTCATTTCCAGCATCATCACAGCCAGCATCAGCAGCAAAGGAGGTCTCCTGCCAGTCCCCA
  TCCCCCACCCTTCACACATAGAAATGCTGCTTTTAACCAGCTGCCTCATTTGGCGAATAATCTTAACAAA
  CCCCCCTCTCCGTGGAGCAGCTACCAGAGTCCGTCACCAACACCCTCCTCTTCCTGGAGCCCGGGCGGTG
  GTGGATATGGTGGCTGGGGAGGTTCCCAAGGCCGAGATCACCGCAGAGGGCTGAATGGTGGAATAACGCC
  CCTGAACTCCATCTCGCCTTTGAAGAAAAATTTTGCAAGCAATCATATTCAGCTCCAGAAGTATGCTCGC
  CCCAGCTCTGCCTTTGCACCTAAATCCTGGATGGAAGATAGCTTGAACAGGGCTGACAACATTTTTCCTT
  TTCCGGATCGCCCCAGGACATTCGACATGCACTCACTGGAGAGTTCACTCATTGACATAATGAGAGCTGA
  AAATGATACCATTAAAGGTCGTCTAAACTATTCATATCCAGGATCCGATAGCTCTCTGCTTATTAATGCA
  AGGACATATGGGCGAAGGAGAGGTCAGTCTTCACTGTTTCCAATGGAAGATGGATTCTTGGATGATGGCC
  GTGGGGATCAGCCTCTTCATAGTGGCCTGGGTTCACCTCACTGCTTCAGTCACCAGAATGGGGAAAGAGT
  GGAACGATATTCTCGAAAGGTGTTTGTAGGCGGATTGCCTCCAGACATTGATGAAGATGAGATCACAGCT
  AGTTTTCGTCGCTTTGGCCCTCTGATTGTGGATTGGCCTCATAAAGCTGAGAGCAAATCCTATTTTCCTC
  CTAAAGGCTATGCATTCCTGCTGTTTCAAGATGAAAGCTCTGTGCAGGCTCTCATTGATGCATGCATTGA
  AGAAGATGGAAAACTCTACCTTTGTGTATCAAGTCCCACTATCAAGGATAAGCCAGTCCAGATTCGGCCT
  TGGAATCTCAGTGACAGTGACTTTGTGATGGATGGTTCACAGCCACTTGACCCACGAAAAACTATATTTG
  TTGGTGGTGTTCCTCGACCATTACGAGCTGTGGAGCTTGCGATGATAATGGATCGGCTATATGGAGGTGT
  GTGCTACGCTGGGATTGATACCGACCCTGAGCTAAAATACCCAAAAGGAGCTGGGAGAGTTGCGTTCTCT
  AATCAACAGAGTTACATAGCTGCTATCAGTGCCCGCTTTGTTCAGCTGCAGCATGGAGAGATAGATAAAC
  GGGTGGAAGTTAAGCCATATGTCTTGGATGATCAGCTGTGTGATGAATGTCAGGGGGCCCGTTGTGGGGG
  GAAATTTGCTCCATTTTTCTGTGCTAATGTTACCTGTCTGCAGTATTACTGTGAATATTGCTGGGCTGCT
  ATCCATTCTCGTGCTGGCAGGGAATTCCACAAGCCCCTGGTGAAGGAAGGCGGTGACCGCCCTCGGCATA
  TTTCATTCCGCTGGAACTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030627 unedited
  GTATTTTGTAATACGACTCACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGGAAAAAA
  AAGCGTGAGACATCAGGTTGTCATTTTTTATTGTGAGATTCTGCTCCTAAAGATAATAAA
  TGGGGGATTACGGGTTTGGAGTGCTAGTGCAAAGCAATACTGGGAATAAATCTGCTTTTC
  CAGTCAGATTCCATCCACATCTGCAGCCTCCACACCATCACCAAAATGCCACCCCCAGCC
  CTGCTGCTTTTATAAATAATAACACAGCTGCCAATGGCAGCAGTGCTGGGTCAGCTTGGC
  TTTTTCCTGCTCCAGCTACCCATAACATTCAGGATGAGATCTTGGGGTCAGAAAAAGCAA
  AAAGTCAGCAACAGGAACAGCAAGACCCCTTAGAAAAGCAGCAGCTTTCCCCAAGTCCAG
  GTCAGGAAGCTGGAATACTGCCTGAAACAGAGAAGGCAAAATCAGAAGAAAATCAAGGGG
  ACAATTCTTCGGAAAATGGCAATGGGAAGGAGAAAATAAGGATCGAATCTCCAGTGTTGA
  CAGGGTTTGATTATCAAGAAGCCACTGNGCTAGGTACTTCAACCCAACCCTTGACATCTA
  GCGCATCGTCTCTTACTGGTTTCAGTAACTGGTCAGCAGCGATAGCGCCTTCCTCCTCTA
  CAATAATCAATGAAGATGCAAGTTTCTTTCACCAGGGAGGCGTCCCTGCTGCTTCCGCTA
  ATAACCGTGCTCTGNTGTCTCAAAATTCCCCCATCATGTCAGCCCTGGCTTTGGAGGCAG
  CTTCTCTTCTCAGATCGGGCCTTTCTCACAGGACCACCCAATCACCCTCATTTCCGCATC
  ATTACACCCGCATTAGCAGCAAAGGAGGTCTCCTGCCAATCCCATACCCCACCCTTCAAC
  CATAGAATGCTGCTTTTACCCCTTGCCCATTTGGCGAAAATCTTAACAACCCCCCTTCCG
  TGGAGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030627 unedited
  NNNNNTTTTTAGCTATGNACCGCGGCCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTT
  TTCTGATGTGGTGGTAACTTTTTGAGGGATAATGAAATTATGTTCAGCCTCAAAACCCTG
  AAAATTAATTATAATGCTGCTCAGTCTTGCTTATGCATTTGTTTGCTCTAACATGCTCTT
  TCCATTAAAAATTGTTAACTTCCTCCATTGCTGTTAAAAAAATTAAAAAGGGATGGAAAA
  AAGAGAGAGACAAAAAAGATGAAAAAAAAAAAAAAAAAGAAAGGAAAAGGCAAAAGCACT
  TCCCTGGGGGAAAAACTGTCAGATAGACCGATAGTGTTATTGTTGCATGGTTATTTTAAT
  AAAAGTATTCTTATATGTTATATTATTAACAATAATTTAATTAAAAACCCAAAATACTCT
  ATTGTTTCATTTCTCTTTTAATATTTCTCTCTCTCCTCTAAGAAAATGTTGCAGGAAGTT
  TGGATCCTCTTGGTTTCCAGCAAAACAAGAGAATAAAACTTCCATGCAACCCGATGGCAA
  TACGAAAAAGGAGTTCATATTGTTTGCAGTGACACAAGAATCTACAAAACACCTATATTA
  TAGGTCATTCTTTTCATTATACTATAGTTAAGTTACAGACTGCTACAAAAGGACATGCCA
  NCGTGAAGAGGTTGAGGAAGGGGTCAAAATGAACAGAAGAGTGCACTTATTCTGAGGCCT
  GAAAATGAGCACTGCAGTTATCCTTTAGTTCCAGCGGAATGAAATATGCCGAGGGCGGTC
  ACCGCCTTTCTTCACCAGGGGCTTGTGGGAATTCCCTGCCAGCACGAGAATGTATAGCAG
  CCCCAGCATATTCACAGTATACTGCAGACAGGTAACATTAGCACAGAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_030627
• Insert Size: 2880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030627.1, NP_085130.1
• RefSeq Size: 6930 bp
• RefSeq ORF: 2190 bp
• Locus ID: 80315
• UniProt ID: Q17RY0
• Cytogenetics: 5q35.2
• Gene Summary: Sequence-specific RNA-binding protein that binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3' UTR (PubMed:24990967). RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism (PubMed:24990967). Regulates activation of unfolded protein response (UPR) in the process of adaptation to ER stress in liver, by maintaining translation of CPE-regulated mRNAs in conditions in which global protein synthesis is inhibited (By similarity). Required for cell cycle progression, specifically for cytokinesis and chromosomal segregation (PubMed:26398195). Plays a role as an oncogene promoting tumor growth and progression by positively regulating translation of t-plasminogen activator/PLAT (PubMed:22138752). Stimulates proliferation of melanocytes (PubMed:27857118). In contrast to CPEB1 and CPEB3, does not play role in synaptic plasticity, learning and memory (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).