DCUN1D4 (NM_015115) Human Untagged Clone

SKU SC107366

DCUN1D4 (untagged)-Human DCN1, defective in cullin neddylation 1, domain containing 4 (S. cerevisiae) (DCUN1D4), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: DCUN1D4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107366 sequence for NM_015115 edited (data generated by NextGen Sequencing); ATGCACTCGGATGCCGCCGCTGTCAATTTTCAGCTGAACTCTCATCTCTCAACACTGGCA; AATATTCATAAGATCTACCACACCCTTAATAAGCTGAACCTAACAGAAGACATTGGCCAA; GACGATCACCAAACAGGAAGTCTGCGGTCTTGCAGTTCTTCAGACTGCTTTAATAAAGTG; ATGCCACCAAGGAAAAAGAGAAGACCTGCCTCTGGAGATGATTTATCTGCCAAGAAAAGT; AGACATGATAGCATGTATAGAAAATATGATTCGACTAGAATAAAGACTGAAGAAGAAGCC; TTTTCAAGTAAAAGGTGCTTGGAATGGTTCTATGAATATGCAGGAACTGATGATGTTGTA; GGCCCTGAAGGCATGGAGAAATTTTGTGAAGACATTGGTGTTGAACCAGAAAACGTAGTT; ATGCTTGTCCTAGCTTGGAAATTGGATGCACAAAACATGGGTTATTTTACTCTACAGGAG; TGGTTAAAAGGAATGACTTCTCTCCAATGTGATACAACAGAAAAACTCAGAAATACTTTG; GATTACTTAAGATCATTCTTAAATGATTCTACAAACTTTAAACTTATTTACAGATATGCG; TTTGACTTTGCACGGCAATCAAAATACAAAGTTATTAATAAAGACCAGTGGTGCAATGTC; CTAGAGTTTAGCAGAACAATTAATCTTGACCTCAGCAACTATGATGAAGATGGAGCATGG; CCAGTTTTGTTGGACGAGTTTGTGGAGTGGTATAAAGACAAACAGATGTCCTAG; ; Clone variation with respect to NM_015115.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015115 unedited; GTCGGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGTCCTCAGCT; TCGAGCCGAGGTGCAGTGAGCTGGTGGGGGGACCGCGAGGCGAGCGCGGGAGCCTGGGCG; GCGAGCCGGGTGTGAGCTGCCTGAAAATGCACTCGGATGCCGCCGCTGTCAATTTTCAGC; TGAACTCTCATCTCTCAACACTGGCAAATATTCATAAGATCTACCACACCCTTAATAAGC; TGAACCTAACAGAAGACATTGGCCAAGACGATCACCAAACAGGAAGTCTGCGGTCTTGCA; GTTCTTCAGACTGCTTTAATAAAGTGATGCCACCAAGGAAAAAGAGAAGACCTGCCTCTG; GAGATGATTTATCTGCCAAGAAAAGTAGACATGATAGCATGTATAGAAAATATGATTCGA; CTAGAATAAAGACTGAAGAAGAAGCCTTTTCAAGTAAAAGGTGCTTGGAATGGTTCTATG; AATATGCAGGAACTGATGATGTTGTAGGCCCTGAAGGCATGGAGAAATTTTGTGAAGACA; TTGGTGTTGAACCAGAAAACGTAGTTATGCTTGTCCTAGCTTGGAAATTGGATGCACAAA; ACATGGGTTATTTTACTCTACAGGAGTGGTTAAAAGGAATGACTTCTCTCCAATGTGATA; CAACAGAAAAACTCAGAAATACTTTGGATTACTTAAGATCATTCTTAAATGATTCTACAA; ACTTTAAACTTATTTACAGATNNATGCGTTTGACTTTGCACGGCATCAAAATACAAAGTT; ATTAATAAAGACCAGTGGNTGCATGTCNNCTAGAGTTAGCAGAACAATTAATCTTGACCT; CAGCACTATGATGAAATGGAGCATGGCCCAGTTTGTGGACGAGTTGGGGGAGTGA;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_015115
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015115.1, NP_055930.1
• RefSeq Size: 4146 bp
• RefSeq ORF: 594 bp
• Locus ID: 23142
• UniProt ID: Q92564
• Cytogenetics: 4q12
• Summary: Contributes to the neddylation of all cullins by transfering NEDD8 from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes which are necessary for the activation of cullin-RING E3 ubiquitin ligases (CRLs).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) has an alternate exon in place of the first exon and lacks an alternate in-frame exon in the 3' coding sequence compared to variant 3. The resulting isoform (2) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform 3.