PKIG (NM_181804) Human Untagged Clone

CAT#: SC107349

PKIG (untagged)-Human protein kinase (cAMP-dependent, catalytic) inhibitor gamma (PKIG), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PKIG
• Synonyms: PKI-gamma
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_181804, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATGGAGGTCGAGTCCTCCTACTCGGACTTCATCTCCTGTGACCGGACAGGCCGTCGGAATGCGGTCC
  CTGACATCCAGGGAGACTCAGAGGCTGTGAGCGTGAGGAAGCTGGCTGGAGACATGGGCGAGCTGGCACT
  CGAGGGGGCAGAAGGACAGGTGGAGGGAAGCGCCCCAGACAAGGAAGCTGGCAACCAGCCCCAGAGCAGC
  GATGGGACCACCTCGTCTTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181804 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGACCGCAGGAGACA
  CGGGGAAGAGACAGAGAGGAGGGTAAAGTGAGAATCCTGCCCGCACCACAGGTCTGGACT
  GCTAACCTTGAATCCTGGTTTCTTAAATTCTGCCTCCGCTTCTGACTGAGGACCACTGGA
  TTTTGAGGAAACTTTGGTCTTAATTCCCGTACTGATTAGTCAACAGTGGAAAATCTGAAG
  AGATGCAAGCAGGAAAAAGAAATTAAACCAGGCCTGAGGAGCGATGCGACAGGCATGATG
  GAGGTCGAGTCCTCCTACTCGGACTTCATCTCCTGTGACCGGACAGGCCGTCGGAATGCG
  GTCCCTGACATCCAGGGAGACTCAGAGGCTGTGAGCGTGAGGAAGCTGGCTGGAGACATG
  GGCGAGCTGGCACTCGAGGGGGCAGAAGGACAGGTGGAGGGAAGCGCCCCAGACAAGGAA
  GCTGGCAACCAGCCCCAGAGCAGCGATGGGACCACCTCGTCTTGAATCTGACCTTGTCCA
  AGAAGGCTGGACGAGAGACCTTCTGTCCCCTCCCAGAGGGGGAACCCTGGCACTGGCCCA
  GCAGCCTCTTCTCTGAGCTCCATGTCCCAGATAAACCAGGCCAGACTGAGAAGGCTCCCC
  AGAGGCCTCTGTGGCCTNCACTCCGGGAAAGCCCTCTGCCCACACCCACAGGCTTCACAT
  TCCCACCACCTTCGCACCGTGCCCAGGTACACTTTCAAGACACTGTTACCACAAGATGTT
  ATTATTTGAGCTGGCGCCGGGACTTGGGGCGGGGCCTGCCCTACAGTGAGCAGCCCAACA
  GGACGCTTCTCTCGCGAGCGGCCCGGCAGGNACCCTGTCCAACACCACACTNCTTTCAGC
  CATNTTNTGGGCCAAACTGCTGTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_181804
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181804.1, NP_861520.1
• RefSeq Size: 1353 bp
• RefSeq ORF: 231 bp
• Locus ID: 11142
• UniProt ID: Q9Y2B9
• Cytogenetics: 20q13.12
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the protein kinase inhibitor family. Studies of a similar protein in mice suggest that this protein acts as a potent competitive cAMP-dependent protein kinase inhibitor, and is a predominant form of inhibitor in various tissues. The encoded protein may be involved in osteogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 4. Variants 1-5 encode the same protein.