TORC2 (CRTC2) (NM_181715) Human Untagged Clone

CAT#: SC107319

CRTC2 (untagged)-Human CREB regulated transcription coactivator 2 (CRTC2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TORC2
• Synonyms: TORC-2; TORC2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107319 sequence for NM_181715 edited (data generated by NextGen Sequencing)
  ATGGCGACGTCGGGGGCGAACGGGCCTGGTTCGGCCACGGCCTCGGCTTCCAATCCGCGC
  AAATTTAGTGAGAAGATTGCGCTGCAGAAGCAGCGTCAGGCCGAGGAGACGGCGGCCTTC
  GAGGAGGTGATGATGGACATCGGCTCCACCCGGTTACAGGCCCAAAAACTGCGACTGGCA
  TACACAAGGAGCTCTCATTATGGTGGGTCTCTGCCCAATGTTAACCAGATTGGCTCTGGC
  CTGGCCGAGTTCCAGAGCCCCCTCCACTCACCTTTGGATTCATCTCGGAGCACTCGGCAC
  CATGGGCTGGTGGAACGGGTGCAGCGAGATCCTCGAAGAATGGTGTCCCCACTTCGCCGA
  TACACCCGCCACATTGACAGCTCTCCCTATAGTCCTGCCTACTTATCTCCTCCCCCAGAG
  TCTAGCTGGCGAAGGACGGTGGCCTGGGGCAATTTCCCTGCAGAGAAGGGGCAGTTGTTT
  CGACTACCATCTGCACTTAACAGGACAAGCTCTGACTCTGCCCTTCATACAAGTGTGATG
  AACCCCAGTCCCCAGGATACCTACCCAGGCCCCACACCTCCCAGCATCCTGCCCAGCCGA
  CGTGGGGGTATTCTGGATGGTGAAATGGACCCCAAAGTACCTGCTATTGAGGAGAACTTG
  CTAGATGACAAGCATTTGCTGAAGCCATGGGATGCTAAGAAGCTATCCTCATCCTCTTCC
  CGACCTCGGTCCTGTGAAGTCCCTGGAATTAACATCTTTCCATCTCCTGACCAGCCTGCC
  AATGTGCCTGTCCTCCCACCTGCCATGAACACGGGGGGCTCCCTACCTGACCTCACCAAC
  CTGCACTTTCCCCCACCACTGCCCACCCCCCTGGACCCTGAAGAGACAGCCTACCCTAGC
  CTGAGTGGGGGCAACAGTACCTCCAATTTGACCCACACCATGACTCACCTGGGCATCAGC
  AGGGGCATGGGCCTGGGCCCAGGCTATGATGCACCAGGACTTCATTCACCTCTCAGCCAC
  CCATCCCTGCAGTCCTCCCTAAGCAATCCCAACCTCCAGGCTTCCCTGAGCAGTCCTCAG
  CCCCAGCTTCAGGGCTCCCACAGCCACCCCTCTCTGCCTGCCTCCTCCTTGGCCCGCCAT
  GTACTGCCCACCACCTCCCTGGGCCACCCCTCACTCAGTGCTCCGGCTCTCTCCTCCTCC
  TCTTCCTCCTCCTCCACTTCATCTCCTGTTTTGGGCGCCCCCTCTTACCCTGCTTCTACC
  CCTGGGGCCTCCCCCCACCACCGCCGTGTGCCCCTCAGCCCCCTGAGTTTGCTCGCGGGC
  CCAGCCGACGCCAGAAGGTCCCAACAGCAGCTGCCCAAACAGTTTTCGCCAACAATGTCA
  CCCACCTTGTCTTCCATCACTCAGGGCGTCCCCCTGGATACCAGTAAACTGTCCACTGAC
  CAGCGGTTACCCCCATACCCATACAGCTCCCCAAGTCTGGTTCTGCCTACCCAGCCCCAC
  ACCCCAAAGTCTCTACAGCAGCCAGGGCTGCCCTCTCAGTCTTGTTCAGTGCAGTCCTCA
  GGTGGGCAGCCCCCAGGCAGGCAGTCTCATTATGGGACACCGTACCCACCTGGGCCCAGT
  GGGCATGGGCAACAGTCTTACCACCGGCCAATGAGTGACTTCAACCTGGGGAATCTGGAG
  CAGTTCAGCATGGAGAGCCCATCAGCCAGCCTGGTGCTGGATCCCCCTGGCTTTTCTGAA
  GGGCCTGGATTTTTAGGGGGTGAGGGGCCAATGGGTGGCCCCCAGGATCCCCACACCTTC
  AACCACCAGAACTTGACCCACTGTTCCCGCCATGGCTCAGGGCCTAACATCATCCTCACA
  GGGGACTCCTCTCCAGGTTTCTCTAAGGAGATTGCAGCAGCCCTGGCCGGAGTGCCTGGC
  TTTGAGGTGTCAGCAGCTGGATTGGAGCTAGGGCTTGGGCTAGAAGATGAGCTGCGCATG
  GAGCCACTGGGCCTGGAAGGGCTAAACATGCTGAGTGACCCCTGTGCCCTGCTGCCTGAT
  CCTGCTGTGGAGGAGTCATTCCGCAGTGACCGGCTCCAATGA
  
  Clone variation with respect to NM_181715.2
  439 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181715 unedited
  AAATATAACACCCGCCCGTTGACGCAAAGGGCGGTAGGCGTGTACGGTGGNGAGGTCTAT
  ATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG
  GCCGCGAATTCGGCACGAGGCTGGGGCCGGGTTCGCGGTGCTCGCTGAGGCGGCGGTGGC
  TACGGCTGGAGGAGCCGGGCCGAGGCCGCGGCGGAGGCCGCGGCTGGTACTGGGAGGGTG
  GCAGGGAGGGACGGGGAAGGAAGATGGCGACGTCGGGGGCGAACGGGCCTGGTTCGGCCA
  CGGCCTCGGCTTCCAATCCGCGCAAATTTAGTGAGAAGATTGCGCTGCAGAAGCAGCGTC
  AGGCCGAGGAGACGGCGGCCTTCGAGGAGGTGATGATGGACATCGGCTCCACCCGGTTAC
  AGGCCCAAAAACTGCGACTGGCATACACAAGGAGCTCTCATTATGGTGGGTCTCTGCCCA
  ATGTTAACCAGATTGGCTCTGGCCTGGCCGAGTTCCAGAGCCCCCTCCACTCACCTTTGG
  ATTCATCTCGGAGCACTCGGCACCATGGGCTGGTGGAACGGGTGCAGCGAGATCCTCGAA
  GAATGGTGTCCCCACTTCGCCGATACACCCGCCACATTGACAGCTCTCCCTATAGTCCTG
  CCTACTTATCTCCTCCCCCAGAGTCTAGCTGGCGAAGGACGGTGGCCTGNGGCAATTTCC
  CTGCAGAGAAGGGGCAGTTGTTTCGACTACCATCTGCACTTAACAGGACAAGCTCTGACT
  CTGCCCTTCATACCAGTGTGATGAACCCCAGTCTCCAGGAACCTACCCAGGCCCCACACC
  TCCCAGCATCCTGCCAGCCGACGTTGGGGGTATCCTTGGATGGTGCACACGGCCCCACAT
  ACCTGCTATTGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_181715 unedited
  NTTTGGGTTTCACTATGNNACCGCGGCCGCATNCTAGGATCGGATTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTGATATGATTTTTTTATTAACATATAATAATATATTTA
  ATAAAAAATAGTATCCACTCTGGCTCTCTCCTCCACTGCAAGGGGAAAAATGGTGAGGAG
  GGGCTGGCACTGCCCACCCCTAGGCATCCGGAAAAACCCTGGTTCCAGGGCTCCCCTCTA
  CCCCTGCTTTCCAACAGGCTCTGACCTCCAGACAGACGGTTCAAAGTTACAAGTGCTTTA
  GGCCCTCCCTTGAGTTCCCCCAGGCCCATCCCTTGCGCAAAATTCAGGGGCCACCTGGAC
  AAACCCCTTGCTTTTTCTCATTCTTGGCATCCTTCATTCATGCTAAAAAGAGGATCTGGG
  GACAGAAAGTAAAGTCTCTACCTGCCAGGGGGAAAGGAGGAAAAGAATGGTGGTGGGGGA
  TGGGGCCCAAAAAGGGGATGGGGATGAGGGGCCCCCATTGGAACCGGGCACTGGGGAATG
  ACTCCTCCCCAGCAAGATCAAGGAGCAGGGCACAAGGGTCACTCCACATGTTTAGCCCTT
  TCAGGCCCAATGGCTCCATGCCCAACTTATTTTTTTGCCCAAACCCTAACTCCCATCCAG
  GTTGGTGACACCCCAAAAGCCCGGCCCCTCCGGGCCCGGGGGTGGTTGCAATCTTCCTTA
  AAGAAACCACCGAAGAAGGGAATCCCTTGGAAAGAAATGATTTAAGGCCCCTGGACCCTT
  GCGCGGGAAACACTGGGGTGCAAATTCTTGGCGGGTTTAAGGGTGGGGGGGATCCTCGGG
  GGGCCCACCCATTGGGCCCTCCACCCCCAAAAATACCACTCCCTCTCAGAAAAACCCCGG
  GGGTACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_181715
• Insert Size: 2750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181715.1, NP_859066.1
• RefSeq Size: 2598 bp
• RefSeq ORF: 2082 bp
• Locus ID: 200186
• UniProt ID: Q53ET0
• Cytogenetics: 1q21.3
• Gene Summary: This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2010]