Cyclin B1 (CCNB1) (NM_031966) Human Untagged Clone

CAT#: SC106758

CCNB1 (untagged)-Human cyclin B1 (CCNB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cyclin B1
• Synonyms: CCNB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC106758 sequence for NM_031966 edited (data generated by NextGen Sequencing)
  ATGGCGCTCCGAGTCACCAGGAACTCGAAAATTAATGCTGAAAATAAGGCGAAGATCAAC
  ATGGCAGGCGCAAAGCGCGTTCCTACGGCCCCTGCTGCAACCTCCAAGCCCGGACTGAGG
  CCAAGAACAGCTCTTGGGGACATTGGTAACAAAGTCAGTGAACAACTGCAGGCCAAAATG
  CCTATGAAGAAGGAAGCAAAACCTTCAGCTACTGGAAAAGTCATTGATAAAAAACTACCA
  AAACCTCTTGAAAAGGTACCTATGCTGGTGCCAGTGCCAGTGTCTGAGCCAGTGCCAGAG
  CCAGAACCTGAGCCAGAACCTGAGCCTGTTAAAGAAGAAAAACTTTCGCCTGAGCCTATT
  TTGGTTGATACTGCCTCTCCAAGCCCAATGGAAACATCTGGATGTGCCCCTGCAGAAGAA
  GACCTGTGTCAGGCTTTCTCTGATGTAATTCTTGCAGTAAATGATGTGGATGCAGAAGAT
  GGAGCTGATCCAAACCTTTGTAGTGAATATGTGAAAGATATTTATGCTTATCTGAGACAA
  CTTGAGGAAGAGCAAGCAGTCAGACCAAAATACCTACTGGGTCGGGAAGTCACTGGAAAC
  ATGAGAGCCATCCTAATTGACTGGCTAGTACAGGTTCAAATGAAATTCAGGTTGTTGCAG
  GAGACCATGTACATGACTGTCTCCATTATTGATCGGTTCATGCAGAATAATTGTGTGCCC
  AAGAAGATGCTGCAGCTGGTTGGTGTCACTGCCATGTTTATTGCAAGCAAATATGAAGAA
  ATGTACCCTCCAGAAATTGGTGACTTTGCTTTTGTGACTGACAACACTTATACTAAGCAC
  CAAATCAGACAGATGGAAATGAAGATTCTAAGAGCTTTAAACTTTGGTCTGGGTCGGCCT
  CTACCTTTGCACTTCCTTCGGAGAGCATCTAAGATTGGAGAGGTTGATGTCGAGCAACAT
  ACTTTGGCCAAATACCTGATGGAACTAACTATGTTGGACTATGACATGGTGCACTTTCCT
  CCTTCTCAAATTGCAGCAGGAGCTTTTTGCTTAGCACTGAAAATTCTGGATAATGGTGAA
  TGGACACCAACTCTACAACATTACCTGTCATATACTGAAGAATCTCTTCTTCCAGTTATG
  CAGCACCTGGCTAAGAATGTAGTCATGGTAAATCAAGGACTTACAAAGCACATGACTGTC
  AAGAACAAGTATGCCACATCGAAGCATGCTAAGATCAGCACTCTACCACAGCTGAATTCT
  GCACTAGTTCAAGATTTAGCCAAGGCTGTGGCAAAGGTGTAA
  
  Clone variation with respect to NM_031966.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031966 unedited
  CAGATTTGTATACGACNACTATAGGCGGCACGCGAATTCGCACGAGTTGGTTTCTGCTGG
  GTGTAAGTCCTTGGCTGGTCGGGCCTCCGGTGTTCTGCTTCTCCCCGCTGAGCTGCTGCC
  TGGTGAATAGGAAGCCATGGCGCTCCGAGTCACCAGGAACTCGAAAATTAATGCCTGAAA
  ATAAGGCGAAGATCAACATGGCAGGCGCAAAGCGCGTTCCTACGGCCCCTGCTGCAACCT
  CCAAGCCCGGACTGAGGCCAAGAACAGCTCTTGGGGACATTGGTAACAAAGTCAGTGAAC
  AACTGCAGGCCAAAATGCCTATGAAGAAAGAAGCAAAACCTTCAGCTACTGGAAAAGTCA
  TTGATAAAAAACTACCAAAACCTCTTGAAAAGGTACCTATGCTGGTGCCAGTGCCAGTGT
  CTGATCCAGTGCCAGATCCAGAACCTGAGCCAGAACCTGAGCCTGTTAAAGAAGAAAAAC
  TTTCGCCTGAGCCTATTTTGGTTGATACTGCCTCTCCAAGCCCAATGGAAACATCTGGAT
  GTGCCCCTGCAGAAGAAGACCTGTGTCAAGCTTTCTCTGATGTAATTCTTGCAGTAAATG
  ATGTGGATGCAGAAGATGGAGCTGATCCAAACCTTTGTAGAGAATATGTGAAAGATATTT
  ATGCTTATCTGAGACAACTTGAGGAAGATCAAGCAGTCAGACCAAAATACCTACTGGGTC
  GGGAAGTCACTGGATACATGAGAGCCATCCTAATTGACTGGCTAGTACAGGTTCAAATGA
  AATTCAAGTTGTTGCAAGAGACCATGTACATGACTGTCTNCTTATAGATCGGTTCATGCA
  GAATAATTGTGTGCCCATAATAGCTGCAGCTGGNTANAGTCACTGCATGTTTATTGCATG
  CAATATGAGATATGTCCCTNCANAATAGAGACTTGCTTTTGTGACGACACACTGTACTAT
  GCACAATCAGAAGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031966 unedited
  NGGGGAATTACTGTGNNACCGCGGCCGCTTNCTAGNGATCGGTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTCACAGATGGCACATGGGGCCAATTTTATTTGGAA
  ATATAGTACTCCAACTCAAGTTTACAAGTTACACCTTTGCCACAGCCTTGGCTAAATCTT
  GAACTAGTGCAAAATTCAGCTGGGGTAGAGGGCTGATCTTAGCATGCTTCGATGGGGCAT
  ACTTGTTCTTGACAGTCATGTGCTTTGTAAGTCCTTGATTTACCATGACTACATTCTTAG
  CCAGGTGCTGCATAACTGGAAAAAAAAATTCTTCAGTATATGACAGGTAATGTTGTAAAG
  TTGGTGTCCATTCACCATTATCCAGAATTTTCAGTGCTAAGCAAAAAGCTCCTGCTGCAA
  TTTGAGAAGGAGGAAAGTGCACCATGTCATAGTCCAACATAGTTAGTTCCATCAGGTATT
  TGGCCAAAGTATGTTGCTCGACATCAACCTCTCCAATCTTAGATGCTCTCCGAAGGAAGT
  GCAAAGGTAGAGGCCGACCCAGACCAAAGTTTAAAGCTCTTAGAATCTTCATTTCCATCT
  GTCTGATTTGGTGCTTAGTATAAGTGTTGGCAGTCACAAAAGCAAAGTCACCAATTTCTG
  GAGGGTACATTTCTTCAATATTGCTTGGCATAAACATGGCAGTGACACCCAACAGCTGCA
  GCATCTTTTTGGGCACACAATTATTCTGGATGAACCGATCAATAATGGAGAACAGTCTGT
  ACATGGTCTCCTGCAACAACCTGGAATTTCATTTTGACCCTGGCCTAGCCAGTCCATTAA
  GATGGCTCTCATGGTTCCAGGGCCTTCCGGACCCAAAGGGATTTTGGCCTGACGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031966
• Insert Size: 1500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031966.2, NP_114172.1
• RefSeq Size: 2101 bp
• RefSeq ORF: 1302 bp
• Locus ID: 891
• UniProt ID: P14635
• Cytogenetics: 5q13.2
• Domains: cyclin_C, CYCLIN, cyclin
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation
• Gene Summary: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).