PDE4 (PDE4B) (NM_002600) Human Untagged Clone

CAT#: SC106296

PDE4B (untagged)-Human phosphodiesterase 4B, cAMP-specific (PDE4B), transcript variant a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE4
• Synonyms: DPDE4; PDEIVB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC106296 sequence for NM_002600 edited (data generated by NextGen Sequencing)
  ATGAAGAAAAGCAGGAGTGTGATGACGGTGATGGCTGATGATAATGTTAAAGATTATTTT
  GAATGTAGCTTGAGTAAATCCTACAGTTCTTCCAGTAACACACTTGGGATCGACCTCTGG
  AGAGGGAGAAGGTGTTGCTCAGGAAACTTACAGTTACCACCACTGTCTCAAAGACAGAGT
  GAAAGGGCAAGGACTCCTGAGGGAGATGGTATTTCCAGGCCGACCACACTGCCTTTGACA
  ACGCTTCCAAGCATTGCTATTACAACTGTAAGCCAGGAGTGCTTTGATGTGGAAAATGGC
  CCTTCCCCAGGTCGGAGTCCACTGGATCCCCAGGCCAGCTCTTCCGCTGGGCTGGTACTT
  CACGCCACCTTTCCTGGGCACAGCCAGCGCAGAGAGTCATTTCTCTACAGATCAGACAGC
  GACTATGACTTGTCACCAAAGGCGATGTCGAGAAACTCTTCTCTTCCAAGCGAGCAACAC
  GGCGATGACTTGATTGTAACTCCTTTTGCCCAGGTCCTTGCCAGCTTGCGAAGTGTGAGA
  AACAACTTCACTATACTGACAAACCTTCATGGTACATCTAACAAGAGGTCCCCAGCTGCT
  AGTCAGCCTCCTGTCTCCAGAGTCAACCCACAAGAAGAATCTTATCAAAAATTAGCAATG
  GAAACGCTGGAGGAATTAGACTGGTGTTTAGACCAGCTAGAGACCATACAGACCTACCGG
  TCTGTCAGTGAGATGGCTTCTAACAAGTTCAAAAGAATGCTGAACCGGGAGCTGACACAC
  CTCTCAGAGATGAGCCGATCAGGGAACCAGGTGTCTGAATACATTTCAAATACTTTCTTA
  GACAAGCAGAATGATGTGGAGATCCCATCTCCTACCCAGAAAGACAGGGAGAAAAAGAAA
  AAGCAGCAGCTCATGACCCAGATAAGTGGAGTGAAGAAATTAATGCATAGTTCAAGCCTA
  AACAATACAAGCATCTCACGCTTTGGAGTCAACACTGAAAATGAAGATCACCTGGCCAAG
  GAGCTGGAAGACCTGAACAAATGGGGTCTTAACATCTTTAATGTGGCTGGATATTCTCAC
  AATAGACCCCTAACATGCATCATGTATGCTATATTCCAGGAAAGAGACCTCCTAAAGACA
  TTCAGAATCTCATCTGACACATTTATAACCTACATGATGACTTTAGAAGACCATTACCAT
  TCTGACGTGGCATATCACAACAGCCTGCACGCTGCTGATGTAGCCCAGTCGACCCATGTT
  CTCCTTTCTACACCAGCATTAGACGCTGTCTTCACAGATTTGGAGATCCTGGCTGCCATT
  TTTGCAGCTGCCATCCATGACGTTGATCATCCTGGAGTCTCCAATCAGTTTCTCATCAAC
  ACAAATTCAGAACTTGCTTTGATGTATAATGATGAATCTGTGTTGGAAAATCATCACCTT
  GCTGTGGGTTTCAAACTGCTGCAAGAAGAACACTGTGACATCTTCATGAATCTCACCAAG
  AAGCAGCGTCAGACACTCAGGAAGATGGTTATTGACATGGTGTTAGCAACTGATATGTCT
  AAACATATGAGCCTGCTGGCAGACCTGAAGACAATGGTAGAAACGAAGAAAGTTACAAGT
  TCAGGCGTTCTTCTCCTAGACAACTATACCGATCGCATTCAGGTCCTTCGCAACATGGTA
  CACTGTGCAGACCTGAGCAACCCCACCAAGTCCTTGGAATTGTATCGGCAATGGACAGAC
  CGCATCATGGAGGAATTTTTCCAGCAGGGAGACAAAGAGCGGGAGAGGGGAATGGAAATT
  AGCCCAATGTGTGATAAACACACAGCTTCTGTGGAAAAATCCCAGGTTGGTTTCATCGAC
  TACATTGTCCATCCATTGTGGGAGACATGGGCAGATTTGGTACAGCCTGATGCTCAGGAC
  ATTCTCGATACCTTAGAAGATAACAGGAACTGGTATCAGAGCATGATACCTCAAAGTCCC
  TCACCACCACTGGACGAGCAGAACAGGGACTGCCAGGGTCTGATGGAGAAGTTTCAGTTT
  GAACTGACTCTCGATGAGGAAGATTCTGAAGGACCTGAGAAGGAGGGAGAGGGACACAGC
  TATTTCAGCAGCACAAAGACGCTTTGTGTGATTGATCCAGAAAACAGAGATTCCCTGGGA
  GAGACTGACATAGACATTGCAACAGAAGACAAGTCCCCCGTGGATACATAA
  
  Clone variation with respect to NM_002600.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002600 unedited
  TCAGCAATTTGTAATACGACCTCACTATAGGGCGGCCGCGAATTCGCACGAGGAAAAATG
  TCAGCAAACTGCATTGAATAACAGACATCCTAAGAGGGGATATTTTCCACCTCTATAATG
  AAGAAAAGCAGGAGTGTGATGACGGTGATGGCTGATGATAATGTTAAAGATTATTTTGAA
  TGTAGCTTGAGTAAATCCTACAGTTCTTCCAGTAACACACTTGGGATCGACCTCTGGAGA
  GGGAGAAGGTGTTGCTCAGGAAACTTACAGTTACCACCACTGTCTCAAAGACAGAGTGAA
  AGGGCAAGGACTCCTGAGGGAGATGGTATTTCCAGGCCGACCACACTGCCTTTGACAACG
  CTTCCAAGCATTGCTATTACAACTGTAAGCCAGGAGTGCTTTGATGTGGAAAATGGCCCT
  TCCCCAGGTCGGAGTCCACTGGATCCCCAGGCCAGCTCTTCCGCTGGGCTGGTACTTCAC
  GCCACCTTTCCTGGGCACAGCCAGCGCAGAGAGTCATTTCTCTACAGATCAGACAGCGAC
  TATGACTTGTCACCAAAGGCGATGTCGAGAAACTCTTCTCTTCCAAGCGAGCAACACGGC
  GATGACTTGATTGTAACTCCTTTTGCCCAGGTCCTTGCCAGCTTGCGAAGTGTGAGAAAC
  AACTTCACTATACTGACAAACCTTCATGGTACATCTAACAAGAGGTCCCCAGCTGCTAGT
  CAGCCTCCTGTCTCCAGAGTCAACCCACAAGAAAATCTTATCAAAAATTAGCAATGGAAA
  CGCTGGAGGAATTAGACTGGGTGTTAGACCAGCTAGAGACCATACAGACCTACCCGTCTG
  TCAGTGAGATGGCTTCTAAACAGNTCAAAGAATGCTGAACCGGNAGCTGACACCCTCTCA
  AAGAGAGCCGATCAGGNAACCAGTGTCTGATACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_002600 unedited
  NNNNCCCTACTGTGNNACCGCGGCCTTATNCNANGATCGNATTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACATGCTTTTCTTTATTCACCTCATAAAA
  AAGTCTCTTTAGAATGCATTTGAACACAATATATAATAATAATACTATAACATACATTGG
  GAGAAACATTTGAAAATGATAAAATGTCCACCTGAAACAATGCAGTGTTCATAAACATAC
  ATTGGTCATGCACATTTGTGCCATTTTCTGTAATTACTGAGTCACAATGCTGAACTGAAA
  GCATGAAACATATTTTACAAACTGAAACTGACATCAGTAAAAGGCCTAACAAGTGTTATC
  AAAAAAGGCTTCACATTTGCGTAATTTGCTACATCAGTTCACATTTTATTATAAAAATTC
  ACATTGGTTTATTGGTTTCTTTTGGAAAGAACTGGAACATGACATTCCTGCTTAATGGCA
  GGCAGGAGGCTACCTCTTCAAAGTCAGTAGTTCGGGAGCATTCAAAAAAATCATCTGTGA
  GCAAATGTAGTGTTTCCTTTTGAACCAAAGCCACAATGATAGTAGGATCCAGTTATAACC
  CAACTCATGCCCACATAAATTTGGATAGAACCTGGGTAAAAACTACATCCCATTGCCCCC
  TGCCCCTATCAAGCTTTACAAAAGTAAAAGCTCAGTATAAACCATTTAGTATCTAAGGTG
  AGAATATTGTTTCCAAAACTGGTTCATAAAGGGGGTGGGCAAAAATAGCAACTCAGGCAC
  GGAAAGGGCAGACCCTTTGAACAAAGCCCCCCCTGTTCTTTCTGGCAAGGGGGGCCTATG
  TCCTTTCCTAAGGACGTTGGGGTTAATAAAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002600
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002600.3, NP_002591.2
• RefSeq Size: 4264 bp
• RefSeq ORF: 2211 bp
• Locus ID: 5142
• UniProt ID: Q07343
• Cytogenetics: 1p31.3
• Domains: PDEase
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. The encoded protein regulates the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (a) encodes the longest isoform (1, also referred to as PDE4B1 or Tm72). Both variants a and d encode the same isoform.