TAT (BC020707) Human Untagged Clone

CAT#: SC106218

TAT (untagged)-Homo sapiens, Similar to tyrosine aminotransferase, clone MGC:22474 IMAGE:4710626, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAT
• Synonyms: tyrosine aminotransferase; tyrosine aminotransferase, cytosolic
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC020707, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACCCATACATGATTCAGATGAGCAGCAAAGGCAACCTCCCCTCAATTCTGGACGTGCATGTCAACG
  TTGGTGGGAGAAGCTCTGTGCCGGGAAAAATGAAAGGCAGAAAGGCCAGGTGGTCTGTGAGGCCCTCAGA
  CATGGCCAAGAAAACTTTCAACCCCATCCGAGCCATTGTGGACAACATGAAGGTGAAACCAAATCCAAAC
  AAAACCATGATTTCCCTGTCCATTGGTGAGTTGGGGACACTACTGAGGGGCTGTCATTGTCCTCCTCTAC
  TCTCATGTTCACAGGCTGGTTGGAGAAGATGGCAGTTGGGGGTGAGTCTGAGTACTGAACATGGGAGAAT
  AACCTCTTGGCTCCTTTTGTGTTTTCCTCCCATAAAAAGGGGACCCTACTGTGTTTGGAAACCTGCCTAC
  AGACCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC020707 unedited
  GCGGTCACATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGCAAANAAA
  AGCTAAGGGAGCTTTCGGGGTTGGCTTCTTGGAGGCTGCTTTCTCCTTTACTTGGAAGGC
  TTCGCTAGTGATGGACCCATACATGATTCAGATGAGCAGCAAAGGCAACCTCCCCTCAAT
  TCTGGACGTGCATGTCAACGTTGGTGGGAGAAGCTCTGTGCCGGGAAAAATGAAAGGCAG
  AAAGGCCAGGTGGTCTGTGAGGCCCTCAGACATGGCCAAGAAAACTTTCAACCCCATCCG
  AGCCATTGTGGACAACATGAAGGTGAAACCAAATCCAAACAAAACCATGATTTCCCTGTC
  CATTGGGGACCCTACTGTGTTTGGAAACCTGCCTACAGACCCTGAAGTTACCCAGGCAAT
  GAAAGATGCCCTGGACTCGGGCAAATATAATGGCTATGCCCCATCCATCGGCTTCCTATC
  CAGTCGGGAGGAGATTGCTTCTTATTACCACTGTCCTGAGGCACCCCTAGAAGCTAAGGA
  CGTCATTCTGACAAGTGGCTGCAGCCAAGCTATTGACCTTTGTTTAGCTGTGTTGGCCAA
  CCCAGGGCAAAACATCCTGGTTCCAAGACCTGGTTTCTCTCTCTACAAGACTCTGGCTGA
  GTCTATGGGAATTGAGGTCAAACTCTACAATTTGTTGCCAGAGAAATCTTGNGAAATTGA
  CCTGAACAACTGGAATATCTAATTGATGAAAAGACAGCTTGTCTCATTGTCAATAATCCA
  TCAAACCCCTGTGGGTCAGTGTTCAGCAAACGTCATCTTCAGAAGATTCTGCAGTGGCTG
  CACGGCAGTGTGTCCCCATCTTAGCTGATGAGATCTATGGAGACATGGTGTTTTTCNGAT
  TTGCAATATGAAACACTGGCCAC
  
• Restriction Sites: NotI-NotI
• ACCN: BC020707
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020707.1, AAH20707.1
• RefSeq Size: 1116 bp
• RefSeq ORF: 1116 bp
• Locus ID: 6898
• Cytogenetics: 16q22.2
• Protein Families: Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism, Ubiquinone and other terpenoid-quinone biosynthesis
• Gene Summary: This nuclear gene encodes a mitochondrial protein tyrosine aminotransferase which is present in the liver and catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Mutations in this gene cause tyrosinemia (type II, Richner-Hanhart syndrome), a disorder accompanied by major skin and corneal lesions, with possible cognitive disability. A regulator gene for tyrosine aminotransferase is X-linked. [provided by RefSeq, Jul 2008]