Lamin B2 (LMNB2) (BC006551) Human Untagged Clone

CAT#: SC106163

LMNB2 (untagged)-Human lamin B2 (cDNA clone MGC:2538 IMAGE:2961134), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Lamin B2
• Synonyms: LAMB2; lamin B2; LMN2; MGC2721
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC106163 sequence for BC006551 edited (data generated by NextGen Sequencing)
  ATGGCCACGCCGCTGCCCGGCCGCGCGGGCGGGCCCGCCACGCCGCTGTCGCCCACGCGC
  CTGTCGCGGCTGCAGGAGAAGGAGGAGCTGCGCGAGCTCAACGACCGCCTGGCGCACTAC
  ATCGACCGCGTCCGCGCGCTGGAGCTGGAGAACGACCGGCTCCTGCTCAAGATCTCAGAG
  AAGGAGGAGGTGACCACGCGCGAGGTGAGTGGCATCAAGGCGCTGTACGAGTCGGAGCTG
  GCCGATGCCCGGAGAGTCCTGGATGAGACGGCTCGAGAGCGTGCCCGGCTGCAGATAGAG
  ATTGGGAAGCTGAGGGCAGAGTTGGACGAGGTCAACAAGAGCGCCAAGAAGAGGGAGGGC
  GAGCTTACGGTGGCCCAGGGCCGTGTGAAGGACCTGGAGTCCCTGTTCCACCGGAGCGAG
  GTGGAGCTGGCAGCTGCCCTCAGCGACAAGCGCGGCCTGGAGAGTGACGTGGCTGAGCTG
  CGGGCCCAGCTGGCCAAGGCCGAGGACGGTCATGCAGTGGCCAAAAAGCAGCTGGAGAAG
  GAGACGCTGATGCGTGTGGACCTGGAGAACCGCTGCCAGAGCCTGCAGGAGGAGCTGGAC
  TTCCGGAAGAGTGTGTTCGAGGAGGAGGTGCGGGAGACGCGGCGGCGGCACGAGCGGCGC
  CTGGTGGAGGTGGACAGCAGCCGGCAGCAGGAGTACGACTTCAAGATGGCACAGGCGCTG
  GAGGAGCTGCGGAGCCAGCACGACGAGCAAGTGCGGCTCTACAAGCTGGAGCTGGAGCAG
  ACCTACCAGGCCAAGCTGGACAGCGCCAAGCTGAGCTCTGACCAGAACGACAAGGCGGCC
  AGTGCGGCTCGCGAGGAGCTGAAGGAGGCCCGCATGCGCCTGGAGTCCCTCAGCTACCAG
  CTCTCCGGCCTCCAGAAGCAGGCCAGTGCCGCTGAAGATCGCATTCGGGAGCTGGAGGAG
  GCCATGGCCGGGGAGCGGGACAAGTTCCGGAAGATGCTGGACGCCAAGGAGCAGGAGATG
  ACGGAGATGCGGGACGTGATGCAGCAGCAGCTGGCCGAGTACCAGGAGCTGCTGGACGTG
  AAGCTGGCCCTGGACATGGAGATCAACGCCTACCGGAAGCTCCTGGAGGGCGAGGAGGAG
  AGGCTGAAGCTGTCCCCCAGCCCATCCTCGCGCGTCACCGTCTCACGAGCCACCTCGAGC
  AGCAGCGGCAGCTTGTCCGCCACCGGGCGCCTGGGCCGCAGTAAGCGGAAGCGGCTGGAG
  GTGGAGGAGCCCTTGGGCAGCGGCCCAAGCGTCCTGGGCACGGGCACGGGTGGCAGCGGT
  GGCTTCCACCTGGCCCAGCAGGCCTCGGCCTCGGGCAGCGTCAGCATCGAGGAGATCGAC
  CTGGAGGGCAAGTTTGTGCAGCTCAAGAACAACTCGGACAAGGATCAGTCTCTGGGGAAC
  TGGAGAATCAAGAGGCAGGTCTTGGAGGGGGAGGAGATCGCCTACAAGTTCACGCCCAAG
  TACATCCTGCGCGCCGGCCAGATGGTCACGGTGTGGGCAGCTGGTGCGGGGGTGGCCCAC
  AGCCCCCCCTCGACGCTGGTGTGGAAGGGCCAGAGCAGCTGGGGCACGGGCGAGAGCTTC
  CGCACCGTCCTGGTTAACGCGGATGGCGAGGAAGTGGCCATGAGGACTGTGAAGAAGTCC
  TCGGTGATGCGTGAGAATGAGAATGGGGAGGAAGAGGAGGAGGAAGCCGAGTTTGGCGAG
  GAGGATCTTTTCCACCAACAGGGGGACCCGAGGACCACCTCAAGAGGCTGCTACGTGATG
  TGA
  
  Clone variation with respect to BC006551.2
  1356 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC006551 unedited
  ACGTCANCATTTGTATACGACNACTATAGGGCGGCCGCGATTCGGCACCAGGGATTGAAT
  GAGCCCGCCGAGCCCGGGCCGCCGTCGGGAGCAGCGCATGCCGCGAGCCGCCGCCACCAT
  GGCCACGCCGCTGCCCGGCCGCGCGGGCGGGCCCGCCACGCCGCTGTCGCCCACGCGCCT
  GTCGCGGCTGCAGGAGAAGGAGGAGCTGCGCGAGCTCAACGACCGCCTGGCGCACTACAT
  CGACCGCGTCCGCGCGCTGGAGCTGGAGAACGACCGGCTCCTGCTCAAGATCTCAGAGAA
  GGAGGAGGTGACCACGCGCGAGGTGAGTGGCATCAAGGCGCTGTACGAGTCGGAGCTGGC
  CGATGCCCGGAGAGTCCTGGATGATACGGCTCGAGAGCGTGCCCGGCTGCAGATAGAGAT
  TGGGAAGCTGAGGGCAGAGTTGGACGAGGTCAACAAGAGCGCCAAGAAGAGGGAGGGCGA
  GCTTACGGTGGCCCAGGGCCGTGTGAAGGACCTGGAGTCCCTGTTCCACCGGAGCGAGGT
  GGAGCTGGCAGCTGCCCTCATCGACAAGCGCGGCCTGGAGAGTGACGTGGCTGATCTGCG
  GGCCCAGCTGGCCAAGGCCGATGACGGTCATGCAGTGGCCAAAAAGCAGCTGGAGAATGA
  GACGCTGATGCGTGTGGACCTGGAGAACCGCTGCCAGAGCCTGCATGATGAGCTGGACTT
  CCGGAATATTGTGTTCNATGATGATGTGCGGGATACNCNGCGGCGGCACGATCGGCGCCT
  GGTGGATGTGGACAGCATCCGGCAGCATGAGTACGACTTCAAGATGCACATGCGCTGGAT
  GATCTGCNGAGCCAGCACGACNAGCCAGTGCGGCTCTACAGCTGGAGCTGGAGCAGACCT
  ACCAGCCCAGCTGGCAGCGCCAGCTGAGCTCTGACAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC006551 unedited
  CTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTGATATAAAAATAGTTTTCAGTGGCTC
  TGGGTAAAGAAAGTGTGTGGATGAAGAGTGTGGGTTCACATCACGTAGCAGCCTCTTGAG
  GTGGTCCTCGGGTCCCCCTGTTGGTGGAAAAGATCCTCCTCGCCAAACTCGGCTTCCTCC
  TCCTCTTCCTCCCCATTCTCATTCTCACGCATCACCGAGGACTTCTTCACAGTCCTCATG
  GCCACTTCCTCGCCATCCGCGTTAACCAGGACGGTGCGGAAGCTCTCGCCCGTGCCCCAG
  CTGCTCTGGCCCTTCCACACCAGCGTCGAGGGGGGGCTGTGGGCCACCCCCGCACCAGCT
  GCCCACACCGTGACCATCTGGCCGGCGCGCAAGATGTACTTGGGCGTGAACTTGTAGGCG
  ATCTCCTCCCCCTCCAAGACCTGCCTCTTGATTCTCCAGTTCCCCAGAGACTGATCCTTG
  TCCGAGTTGTTCTTGAGCTGCACAAACTTGCCCTCCAGGTCGATCTCCTCGATGCTGACG
  CTGCCCGAGGCCGAGGCCTGCTGGGCCAGGTGGAAGCCACCGCTGCCACCCGTGCCCGTG
  CCCAGGACGCTTGGGCCGCTGCCCAAGGGCTCCTCCACCTCCAGCCGCTTCCGCTTACTG
  CGGCCCAAGCGCCCGGTGGCGGACAAGCTGCCGCTGCTGCTCGAGGTGGCTCGTGAGACG
  GTGACGCGCCGAGATGGGCTGGGGGACAGCTTTAGCCTCTCCTTCTTGCCCTCCAGGAGC
  TTCCGGTAGCGTTGATCTCCATGTCCAAGG
  
• Restriction Sites: NotI-NotI
• ACCN: BC006551
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006551.2, AAH06551.1
• RefSeq Size: 1943 bp
• RefSeq ORF: 1803 bp
• Locus ID: 84823
• Cytogenetics: 19p13.3
• Domains: IF_tail, filament
• Gene Summary: This gene encodes a B type nuclear lamin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Mutations in this gene are associated with acquired partial lipodystrophy. [provided by RefSeq, May 2012]