GNS (BC017742) Human Untagged Clone

CAT#: SC105874

(untagged)-Homo sapiens, clone IMAGE:4391536

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GNS
• Synonyms: G6S
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC017742, the custom clone sequence may differ by one or more nucleotides
  
  
  AGAGCAGTAAGCTTGTGATAAAGGCCAATTCCAGGTAGCTCTTGAAGGTGATAGCCATCTACTTTCCAGT
  GGCTGCCAACCACAGGGAGTGCCAGTTAACACTGGAAGGATTAAGGCAAGGTCCCTTCTCTTGAGACTCC
  CCTCTGAGATCTGAAAAATGAAGTGGCTTAGGAACATCAGCAGTGAAGAACTGCCAAGAGTTGGTGAAGG
  TTGTCTCTTCCGAGGGCCTTCTGAAGACAGGGCTCTTGAACAGACAAGTGGAAGGGCTGTACCAGGGATA
  AAGGAAAGAAGTGCCTGTCCAGCAGGGAGCTTGAATTTAAGTTCCATGTATGAAGTCATTGGCTCTATCT
  GCATTTTTCTGTCATTCTCTTCATTTGTTTTAAGGTGGAAAATTTTCTTACAGTTGATGCAAAGTATCAA
  CTACTTTACCCTACCTTCTCCCCTTTTAGATGGGTTCTTCCTGAGTTTTGGAGTCTTGTATGATTATCAG
  TATTCCCCTGTCAAAATCAAATCTATTCAGGTTTCTTCACTGTTGAGAACACCTAAATGTTTTTATTTTT
  GAGAAGTGGGGACAGAGTCTCACTATGTCACCCAGGCTGGAGTGCAATGGCATGATCTCAGCTCACTGCA
  ACCTTCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCCGCCTCCTGAGTAGCTGGGATTATAGGCACGCA
  CCACCACGCCCAGCTAATTTTTTGTATTTTTAGTAGAGACAGAGTTTCACCATGTTGGCCAGGCTGGTCT
  TGAACTCCTGACCTTGTGATCCACCCACCTCGGCCTCCCAGAGTGCTGGGATTACAGGCATGAGCCACCA
  CGCTTGGCTAAGAACACCTAAATTTTTATGTTTCTTGGCTCAAAAACCAGTTCCATTTCTAATGTTGTCC
  TCACAAGAAGGCTAATTGGTGGTGAGACAGCAGGGGAGGAGGAAGAGCTGTGGTTTGTAACTTGTTCAAC
  TCAGGCAATAAGCGATTTTAGCTTTATTTAAAGTCTTCTGTCCAGCTTTAAGCACTTTGTAAGACATGGC
  TGAAAGTAGCTTTTCTATCAGAATTGCAGATAGTCATGTTGGGCTAACAGTCAATTGGATATATTCCTTT
  ACCTCACATGACCCCAGCAACTGTGGTGGTATCTAGAGGTGAAACAGGCAAGTGAAATGGACACCTCTGC
  TGTGAATGTTTTAGAGAAGGAAATTCAAAAAATGTTGTAACTGAAAGCACTGTTGAATATGGGTATCGGC
  TTTCTTTTTCACTTTGACTCTTAACATTATCAGTCAACTTCCACATTAATGAAAGTTGACCATAGTTATT
  TCCAAATAAAAAGAAACCAACTCTTACCAGGTCTTGGACTGTGATGTCATATTATTCAGTTTTATGCTTG
  TTCCTGAGCAGAACTCATAAGAGTGACATAGTCAGCTGCTGACGGCACCTCAGCCACGCCACTCTTACTC
  AGTTCAGTGGGTGTGCTTGCGTGGTAGGATGTGGTGCAGCCCTCTCTACGCTCTTCTATTTTTGGTATAT
  TTCCTATCTAACCTTCAAATAGCTTCCAATTCTTTTTTTCTTGGACTGGCTTCATTCTGAATTTGTGCTA
  AAATAATCTTTCATAAAGAGACCTCAGTTTATAGCGTAACAGACTACACAATGCACTGATGTTTTCATAA
  TGTTTAAGGGACCCACTGCAAGAAGCTTGCTGCCTCCTTTTAATTGTATTCATTTAGATTTTGATTTTCC
  ATGTTAAGAAGGTGAGGTCCATGTTGGTGCCCTTCAGAGTAGAGAACCATGTAAACATTAGGAATGAACA
  GAGGCCTTAGGAATGAATAGAGAGTTTGCCTTATACAATTTCCTGTTACAAAGCTCTCCCTCTCATGCAA
  AGTAGGGAACACCTTTTGAGCATCTTTGAATTTGACAAATGGTGCTGTTGCAAACACTTTTTTTTTGAGA
  TGAAGTCTCGCGGTTGTCACCCGGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAACTTCCACCTC
  CTGGGTTCCAGCAGTTCTCCTGCCTCAGCCTCCCAAGTAGCTGAGATTACAGGCGCCTGCCACCCCACCT
  GGCTGATTTTTGTAATTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGATTAACTCCTGACCTC
  AGGTGATCCACCTTTCTCGGCCTCCCAAAGTGCTGGGATTACGGGTGTGAGCCACCGTGCCCGGCCTGCA
  AACACATTTTAATTGACAACACTAGGGCTGTTGTACAAAATAGTAATGATAGCCATGGAAGTTTTACCTT
  ATTCTGTGAGAAGTGTTCTTAAACTTATTAAGTGTCTAAACTAAGGTTTAGTGCTTTTTTAAAGGAAAGT
  TGTCCCAGGATTCATCCTAAAGAAAGCAAAAGTTAATTCAACTGATCCACCAATGGAATTAGATGGGTAG
  AGTTGGGTTCTTGAGTTTTACCACCACTTAGTTCCCACTGAATTTTGTAACTTCCTGTGTTTGCATCCTC
  TGTTCCTATTCTGCCCTTGCTCTGTGTCATCTCAGTCATTTGACTTAGAAAGTGCCCTTCAAAAGGACCC
  TGTTCACTGCTGCACTTTTCAATGAATTAAAATTTATTTCTGTTCTAAAAAAAAAAAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC017742 unedited
  GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTAGGCTGGT
  GCTGCACTGAAATAGAGCAGTAAGCTTGTGATAAAGGCCAATTCCAGGTAGCTCTTGAAG
  GTGATAGCCATCTACTTTCCAGTGGCTGCCAACCACAGGGAGTGCCAGTTAACACTGGAA
  GGATTAAGGCAAGGTCCCTTCTCTTGAGACTCCCCTCTGAGATCTGAAAAATGAAGTGGC
  TTAGGAACATCAGCAGTGAAGAACTGCCAAGAGTTGGTGAAGGTTGTCTCTTCCGAGGGC
  CTTCTGAAGACAGGGCTCTTGAACAGACAAGTGGAAGGGCTGTACCAGGGATAAAGGAAA
  GAAGTGCCTGTCCAGCAGGGAGCTTGAATTTAAGTTCCATGTATGAAGTCATTGGCTCTA
  TCTGCATTTTTCTGTCATTCTCTTCATTTGTTTTAAGGTGGAAAATTTTCTTACAGTTGA
  TGCAAAGTATCAACTACTTTACCCTACCTTCTCCCCTTTTAGATGGGTTCTTCCTGAGTT
  TTGGAGTCTTGTATGATTATCAGTATTCCCCTGTCAAAATCAAATCTATTCAGGTTTCTT
  CACTGTTGAGAACACCTAAATGTTTTTATTTTTGAGAAGTGGGGACAGAGTCTCACTATG
  TCACCCAGGCTGGAGTGCAATGGCATGATCTCAGCTCACTGCAACCTTCGCCTCCTGGGT
  TCAAGCGATTCTCCTGCCTCCGCCTCCTGAGTAGCTGGGATTATAGGCACACACCACCAC
  GCCCAGCTAATTTTTTGTATTTTTAGTAGAGACAGAGNTTCACCATNGTGGCCAGNCTGG
  TCTTGAACTCCTGACN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC017742 unedited
  ATTTACTTGNACCGCGGCCCGCTATCTAGGATCGAGTTTTTTCTTTTTTTTTTTAGACAG
  AAATATTTTATTCTTGAAAATGCAGCAGTGAACAGGGTCCTTTTGAAGGGCACTTTCTAA
  GTCAAATGACTGAGATGACACAGAGCAAGGGCAGAATAGGAACAGAGGATGCAAACACAG
  GAAGTTACAAAATTCAGTGGGAACTAAGTGGTGGTAAAACTCAAGAACCCAACTCTACCC
  ATCTAATTCCATTGGTGGATCAGTTGAATTAACTTTTGCTTTCTTTAGGATGAATCCTGG
  GACAACTTTCCTTTAAAAAAGCACTAAACCTTAGTTTAGACACTTAATAAGTTTAAGAAC
  ACTTCTCACAGAATAAGGTAAAACTTCCATGGCTATCATTACTATTTTGTACAACAGCCC
  TAGTGTTGTCAATTAAAATGTGTTTGCAGGCCGGGCACGGTGGCTCACACCCGTAATCCC
  AGCACTTTGGGAGGCCGAGAAAGGTGGATCACCTGAGGTCAGGAGTTAATCAGCCTGGCC
  AACATGGTGAAACCCCGTCTCTACTAAAATTACAAAAATCAGCCAGGTGGGGTGGCAGGC
  GCCTGTAATCTCAGCTACTTGGGAGGCTGAGGCAGGAGAACTGCTGGAACCCAGGAGGTG
  GAAGTTGCAGTGAGCCGAGATCACGCCACTGCACTCCAGCCCGGGTGACAACCGCGAGAC
  TTCATCTCAAAAAAAAAGTGTTTGCAACAGCACCATTTGTCAAATTCAAAGATGCTCAAA
  AGGTGTTCCCTACTTTGCATGAGAGGGAGAGCTTTGTAACAGGGAATTGTATAAGGCAAA
  CTCTCTATTCATTCCTAAGGCCTCTNGTCATTCCTATGTTTACATGGNTCTCTACTCTGA
  AGGGCACCACATGGACCTCACCTTCTAC
  
• Restriction Sites: NotI-NotI
• ACCN: BC017742
• Insert Size: 2750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC017742.1
• RefSeq Size: 2651 bp
• RefSeq ORF: 2651 bp
• Locus ID: 2799
• Cytogenetics: 12q14.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycosaminoglycan degradation, Lysosome, Metabolic pathways
• Gene Summary: The product of this gene is a lysosomal enzyme found in all cells. It is involved in the catabolism of heparin, heparan sulphate, and keratan sulphate. Deficiency of this enzyme results in the accumulation of undegraded substrate and the lysosomal storage disorder mucopolysaccharidosis type IIID (Sanfilippo D syndrome). Mucopolysaccharidosis type IIID is the least common of the four subtypes of Sanfilippo syndrome. [provided by RefSeq, Jul 2008]