SIAH1 (AK094663) Human Untagged Clone

CAT#: SC105440

(untagged)-Human cDNA FLJ37344 fis, clone BRAMY2021139, highly similar to Seven in absentia

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIAH1
• Synonyms: SIAH1A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC105440 sequence for AK094663 edited (data generated by NextGen Sequencing)
  ATGGTTATAATTATTTTTCTCCTGCCTCCTTATGTATTTATTTCAGAAATGAGCCGTCAG
  ACTGCTACAGCATTACCTACCGGTACCTCGAAGTGTCCACCATCCCAGAGGGTGCCTGCC
  CTGACTGGCACAACTGCATCCAACAATGACTTGGCGAGTCTTTTTGAGTGTCCAGTCTGC
  TTTGACTATGTGTTACCGCCCATTCTTCAATGTCAGAGTGGCCATCTTGTTTGTAGCAAC
  TGTCGCCCAAAGCTCACATGTTGTCCAACTTGCCGGGGCCCTTTGGGATCCATTCGCAAC
  TTGGCTATGGAGAAAGTGGCTAATTCAGTACTTTTCCCCTGTAAATATGCGTCTTCTGGA
  TGTGAAATAACTCTGCCACACACAGAAAAAGCAGACCATGAAGAGCTCTGTGAGTTTAGG
  CCTTATTCCTGTCCGTGCCCTGGTGCTTCCTGTAAATGGCAAGGCTCTCTGGATGCTGTA
  ATGCCCCATCTGATGCATCAGCATAAGTCCATTACAACCCTACAGGGAGAGGATATAGTT
  TTTCTTGCTACAGACATTAATCTTCCTGGTGCTGTTGACTGGGTGATGATGCAGTCCTGT
  TTTGGCTTTCACTTCATGTTAGTCTTAGAGAAACAGGAAAAATACGATGGTCACCAGCAG
  TTCTTCGCAATCGTACAGCTGATAGGAACACGCAAGCAAGCTGAAAATTTTGCTTACCGA
  CTTGAGCTAAATGGTCATAGGCGACGATTGACTTGGGAAGCGACTCCTCGATCTATTCAT
  GAAGGAATTGCAACAGCCATTATGAATAGCGACTGTCTAGTCTTTGNCACCAGCATTGCA
  CAGCTTTTTGCAGAAAATGGCAATTTAGGCATCAATGTAACTATTTCCATGTGTTGA
  
  Clone variation with respect to AK094663.1
  827 c=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK094663 unedited
  GTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGATTCTCTTGTATAATTTAAG
  TGATCTCTGGATTACTTAATACAATGTAAACAATATGTAAATAGTTGTTATAGACTGTAT
  TTTAAAAATTTTGTATTCTTTATAAATTTTTCTGAATATTTTCAATCCATGGCTGGTGAA
  GTCCTCGGATGCAGACCGTGTGGATACAGAGTGCCGATTTTATACAGGAGTTCACCTGTA
  ACTCCCTGTACCTATCAACAGCTGACTCCAAATTAGAAAGAAATAGAGTAAGGGAGCCTC
  AGGGAGAGTCCTAGCAAAACGGATTCGATTAAACTTCAGTTCCTTGTATAGTTTCTTTAG
  TTGTTTATGGTCCATTTTCTATTTTAGCATTTATTATTCTATGTAGTCTATCCAAAGACG
  ATTAAGGGAGTTCCACATGTTTTCCGGAACATTTTGAAAAGAGAGCTTATCCAGTGTACA
  GATCCTAATAAAGTGCACATTCAGTGTAATTTTATTTTTTTAATATCTTTTTTAATCCTA
  TTTTTCTTCCTCTTTTGCTCAGTAAATTTTGTATGAAACTTTAAAAGGACTTATGGCATG
  TAAACATTATTTATAAAGTAAGTCATGGTTATAATTATTTTTCTCCTGCCTCCTTATGTA
  TTTATTTCAGAAATGAGCCGTCAGACTGCTACAGCATTACCTACCGGTACCTCGAAGTGT
  CCACCATTCCCAGAGGTGCCTGCCCTGACTGGCACAACTGCATCCAACANTGACTTGGCG
  AGTCTTTTTGAGTGTCCAGTCTGCTTTGACTATGTNGTACCGNCCATTCTTCATGTCAGA
  GTGCCATCTTGTTTGTAGCAACTGTCGCCCAAGCTCACATGTNGTCCAACTGCCNGGGCC
  NNTTTGGATCCANCGCACTTTGCTTNGAGAAAGTGCTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK094663 unedited
  CTATGTACTCGGCCGCAATCGAGAGTCGAGTTTTTTCTTTTTTTTTTTTTAATTTGCATT
  TTTTTTTTTTAATGTATAATTATGAAAGACCAAAAACATTTTTTGCAAACTGCCTTTTTA
  AACAAATGATTTGCTTTTAATTACAAATACTGTGCATGACGATGCCTTCTTCTGCAAAAC
  CAATAAATACAAGAACAAATTTTAAAATATGTGATTTGTCCAAGATATCTGAAAAATGAA
  ACAAAAGCCTGATTTGCAGTATTTATAAAAATCAATTTATTCTCTAACCTTTCAAAGATT
  AAGCTCAAAGGTGTGACTCATCTGTAGAAGACAGAAAAGAGAGCACCTTATAGATGCTTT
  AAATAGCTTTTGATTATTTTTCCGATGTCACCTTTACTTTCTGGAACTCATGGCTTCACA
  AAATAGCTGATTTTAAAATATTTCTAATGAACTGATTAATGGGGGAAAAGAAAATATTGA
  ACACGTTATAAATTTTTTACCATAAACAAATATGCATATCAAAAGATACTAACTTTAAAA
  TGGTACAAAAAAAGGAAAAACCTGAATTACAGAAAAGTCGAATTTATTTAATGAAAAACT
  AAAATTAATAAAAATTAGCCNATTCTCACAAAAGAAGCACACAGCAGCACTATGTATTGA
  CTCACAAAGGGAAAAGCAGTGGCCCATGACCACTTCAGATGGCTTGTCNGTTTAAGGAGA
  AAAACCCTAACACGCACACTCCCTCGCAGGCTCACACTCCCACGCAAAAACAAACTTTTC
  AAAATACATCAATCTCCACAAAACAAACTCAGATTATTTACAAGCTTCTTTCTAAACAAA
  GAGCCATCTGGGTGAAAACAATATTTTCAGGGTTACTGGGCTTATTTAAATATAAGTACT
  CTGCACGTTCCGGTTAAACCTTTTTTA
  
• Restriction Sites: NotI-NotI
• ACCN: AK094663
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK094663.1, BAG52904.1
• RefSeq Size: 2829 bp
• RefSeq ORF: 2829 bp
• Locus ID: 6477
• Cytogenetics: 16q12.1
• Domains: Sina
• Protein Families: Druggable Genome
• Protein Pathways: p53 signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway
• Gene Summary: This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]