NDUFS8 (AK002110) Human Untagged Clone

CAT#: SC105006

(untagged)-Human cDNA FLJ11248 fis, clone PLACE1008696, highly similar to Human NADH dehydrogenase-ubiquinone Fe-S protein 8 23 kDa subunit gene

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFS8
• Synonyms: CI-23k; CI23KD; TYKY
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK002110, the custom clone sequence may differ by one or more nucleotides
  
  
  AAGTAGCGGCGGCGCTTCAAGATGCGCTGCCTGACCACGCCTATGCTGCTGCGGGCCCTGGCCCAGGCTG
  CACGTGCAGGACCTCCTGGTGGCCGGAGCCTCCACAGCAGTGCAGTGGCAGCCACCTACAGTGAGTACCT
  GGGTGGCCTCTGGCCTCAGGTGTTCCTGACTGGTCCTGCTGGAGTAGGGGAAGAGACCTGAGTTCCAGTC
  CTGGCTTTGTCAGGGGCGTTCTTCTCTGGGCCTGTTTCCACTTTTAAAATGATTATGGTGATGGAGACTG
  ACTCTGATTCACAGGGGCAGGGTCCATCATTGCCGAGGTTAGCATGTGTGATCTCAGAGCTCCCTGGGTG
  TGATGGGGAGGGGGTTGCCAGGTGTGAGCCATGGGTACCTGTGCCTATTGCCAGTCCCTGGTGAAGGCAA
  AGGCTCAAGCCCCCCACCTACCCCCCTTGATTCCTCTTTCAAGCCCTGTTGGGCAGGGAAGTGCGTCTGG
  GCTCCAATGGCCACCCAAGCACAGGGAGAGGTAGGCACGGACGGGCTCAGGCCAGAGGCGGCCTCCAGGG
  TCCTTGCTGAGGCTGGGGAGAGGTGTGGTGAGTGTACAGCCCCTTTGTAGCCCAGCAGTTGCACCTGGAA
  GTGAGGGCCAGGCTGCTCTCCCTGAGGCTCCAGGGAGACAGTGTGTGAGGCCTCTTGCCATGGCCTCCCT
  GCCCGCCTCTCTGCAGAGTATGTGAACATGCAGGATCCCGAGATGGACATGAAGTCAGTGACTGACCGGG
  CAGCCCGCACCCTGCTGTGGACTGAGCTCTTCCGAGGTGCGTCCTGGGCAGGAGGGGACAGGGAAGGTGC
  CGGGTGGGCACGGATGCATGGGGGAGGAGGGTGCCCCTGCCCACCACACCCGTGCTGCCCACAGGCCTGG
  GCATGACCCTGAGCTACCTGTTCCGGGAACCGGCCACCATCAACTACCCGTTCGAGAAGGGCCCGCTGAG
  CCCTCGCTTCCGTGGGGAGCATGCGCTGCGCCGGTACCCATCCGGGGAGGAGCGTTGCATTGCCTGCAAG
  CTCTGCGAGGCCATCTGCCCCGCCCAGGTGAGCCCCTGCCCCAACCGGCCACCACGCCAGCCCCTGCCAC
  TGGGAGAGGGAGGCCAGGCAGCCCTAGGCCCAAACCCTAGCCCCTGCTTGATGTGCGTCCCCAGGAAGTC
  CCTTTCCCCCTCTGAGCCACTTCCCTCGTGAATGGGAATGATGAGGGCTTGTTACCAGAGCACAGAGTCA
  GTGAGTTGGCAGAGTGAAGCTTCCAGCCCTGGGCAGACGCCTGACACACAGAGGTTCCCCCTTGGGGTCT
  GGCCAAGGGTGTGACCCCTGGGCCTCTGAGCCATGGGGTGACACCTGAGGCCATCCACGCCTCCCTGGAT
  GTAGCCACCGTTTCCGGAGTCCTGCTTGTGTGCCTGTTGCAGCTTCCCTTCCCCAGTGGCTGCTCCCCTG
  AGGCCCGGCTTTCCGTTCTCTTAGTGGGTCTCGTGCTTACCCGTGGGGGCCAGGCCTGCCAGCACGGGAA
  CAATATGACTCGGTCAGAGCTCTGGTCAGACCCCTGTGTGTGCCTGGCAGGAGCCCCGGCAGCCGCCGTC
  GCTACTTTCTGTGTGCACAGCAACCCCTCCCAGTGTTCTGTGACTCGGGTGGCTCCTGTCCAGATCCTAG
  TTTGTCCTCTGGAGCACTCCCAGGTCCTCCAGGCCATGAGCTGCGGCCCTGATGTCTCTACTCCAGCCAC
  GGACTGAGAGTGCATAGGAGTGTCCCCAAACAGCAGTCACGATGGTCACAACCTTGGCTTCCAGACAGTG
  AGTGCTGTCCACAGACAGACCCGTTGCTGTGCCATGTGTGTGACTCACAGCCTCTCCTGTAATCTCCACA
  ACCCCTCGAGGAGGTCGAGGTTATTGTCTCCATCTTTATAAGCACTAAAGGATGAGCCTGCCAGCCACAG
  GTCCCACTGTGAGTTGTGGACACTACATTTCTGGGATGGGGGCATATGAGAGTCCTTGCCAAGCACATTT
  GAAGGTTTTCCCAGCCAGCCCCTCCCCCAGCGGGCTACCCAGGAACTGAGAGTTAGGACGTGCCCTTGAG
  TTTGGGCTGTGCCCCCACTTGCTGTGTAGCTTTGGGGAGCCCCTCAACCTCTCGGAACCTTGTTTTTCTC
  ATCCATCAGATGGAGAGAAGTAGATTTAAGGCACAGGATTGCACCCAACAGTGTAGGCATTCTTGGCGAT
  GTTGCAGGTTCGGCTCCAGACCATCGGGGTAAACTGAGTCACACAGCTGTTTTGGTTTTCCAGTGCATTT
  AAAAGTTAGATTTAGACCGGGCACAGTGGCTCATGCCTGTAATTCCAGCACTTTGAGAAGCCGAGGTGGG
  CGGATCACTTGAGGTCAAGAGTTCGAGACCAGCCTGGCCAACATAGTGAAACCCCGTCTCTACTAAAAAT
  AACAAAACTTAGCCGGGCACGTTGGCAGGTCCCTGTAATCTCAGCTACTTGCTAGGCTGAGGCACGATAA
  TCACTTGAACCGGGAGGTGGGGGTTGCAGTGAGCCAAAATTGAAAAAAAAAAAAAATTAGGGCCGGTTGC
  GGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCGGATCACAAGGTCAGGAATTCAA
  GACCAGCCTGGCCAACATGTTGAAACCCCGTCTCTAGTAAAAATACAAAAATTAGCCGGGTGTGGGGTTG
  CACACCTGTAGTCCCAGCTACTCAAGAGGCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCGGAGGTTG
  CAGTGAACTATGATTGCACCACCATACTCCATCCTGGGCCACAGAGCAAGACTTCGTCTCAAAAAAAAAA
  AAGGATTTACACTGTACTGTAGGCTGCGAAGTGTCTAAAACACAATGTATATACCTTAATTAAAAATACT
  TTAGGGGCCAGGCACAGTGGCTCACACCTGTAGTCCAAGTGCTTTGGGAGGTCAAGGTGAGGGGATTACT
  TGAGCCCAGGAGTTTGAGACCAGCCTGGGCAACATGGCAAAACCCCGACTCTACAACAACAAC
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK002110 unedited
  CCCCCTTCNGGATTACGTATACGATTCACTATAGGCGGCCGCGCAATCGGCACGAGGGCA
  AGGCAAGTAGCGGCGGCGCTTCAAGATGCGCTGCCTGACCACGCCTATGCTGCTGCGGGC
  CCTGGCCCAGGCTGCACGTGCAGGACCTCCTGGTGGCCGGAGCCTCCACAGCAGTGCAGT
  GGCAGCCACCTACAGTGAGTACCTGGGTGGCCTCTAGCCTCAGGTGTTCCTGACTGGTCC
  TGCTGGAGTAGGGGAAGAGACCTGAGTTCCAGTCCTGGCTTTGTCAGGGGCGTTCTTCTC
  TGAGCCTGTTTCCACTTTTAAAATGATTATGGTGATGGAGACTGACTCTGATTCACAGGG
  GCAGGGTCCATCATTGCCGAGGTTAGCATGTGTGATCTCAGAGCTCCCTGGGTGTGATGG
  GGAGNGGGGTTGCCAGGTGTGAGCCATGGGTACCTGTGCCTATTGCCAGTCCCTGGTGAA
  GGCAAAGGCTCAAGCCCCCCACCTACCCCCCTTGATTCCTCTTTCAAGCCCTGTTGGGCA
  GGGAAGTGCGTCTGGGCTCCAATGGCCACCCAAGCACAGGGAGAGGTAGGCACGGACGGG
  CTCAGGCCAGAGGCGGCCTCCAGGGTCCTTGCTGAGGCTGGGGAGAGGTGTGGTTGAGTG
  TACAGCCCCTTTGTACCCCAGCAGTTGCACCTGGAAGTGAGGGCCAGGCTGCTCTCCCTG
  AGGCTCCAGGGAGACAGTGTTGTGCAGGCCTCTTTGCCATGGCCTCACTTGCCCGCCTCT
  CTGCAGAGTATGTGAACATGCCANGATCCCTGAGATGGACCATGAAATCACTGACTTGAC
  CGGGCAGCCCCGCACCCTGCTGGGGGACTGAGCTCTTTCCGAGGCCTGGGCATGCACTCT
  GAGCTACCTTGNTTCCGGGAACCCGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK002110 unedited
  NGGCCTCCTTAGNACCGCGGCCGCATTCTAAGATCGGTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTAACAGGGGGACCTGGGGCTGGCAGGCTCATCCT
  TTAGGGCTTATAAAGATGGAAACAATAACCTCGACCTCCTCGAGGGGTTGGGGAAATTAC
  AGGAAAGGCTGTGAGTCACACACATGGCACAACAACGGGTCTGTCTGTGGACAGCACTCA
  CTGTCTGGAAGCCAAGGTTGGGACCATCGGGACTGCTGTTTGGGGACACTCCTATGCACT
  CTCATTCCGTGGCTGGAGTAAAAACATCAGGGCCGCAGCTCATGGCCTGGAGGACCTGGG
  AGTGCTCCAAAGGACAAACTAGGATCTGGACAGGAGCCACCCGAGTCACAAAACACTGGG
  AGGGGTTGCTGTGCACACAAAAAGTANCGACGGCGGCTGCCGGGGCTCCTGCCAGGCACA
  CACAGGGGTCTGACCAAAGCTCTGACCGAGTCATATTGTTCCCGTGCTGGCAGGCCTGGC
  CCCCACGGGTAAGCACGAGACCCACTAATATAACGGAAAGCCGGGCCTCAGGGGAGCAGC
  CACTGGGGAAGGGAAGCTGCAACAGGCACACAAGCAGGACTCCGGAAACGGTGGCTACAT
  CCAGGGAGGCGTGGATGGCCCTCAGTGTCACCCCATGGCTCAAAGGCCCCAGGGTCAACC
  CTTGGCCAGACCCNAGGGGGAACCTCTGGGTGTTAGGCGCCTGCCCAGGGCTGGAAGCTT
  ACTCTGCCCAATCACTGACTTTGGGCTCTGGGAAACAGCCCTCATAATTCCCATTCACGA
  GGAAGTGGCCCCAAAAGGGGGAAAGAGGACTTCCCCGGGGC
  
• Restriction Sites: NotI-NotI
• ACCN: AK002110
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK002110.1
• RefSeq Size: 3073 bp
• RefSeq ORF: 3073 bp
• Locus ID: 4728
• Cytogenetics: 11q13.2
• Domains: fer4
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. [provided by RefSeq, Mar 2010]