DGAT2 (NM_032564) Human Untagged Clone

SKU SC104559

DGAT2 (untagged)-Human diacylglycerol O-acyltransferase 2 (DGAT2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DGAT2
• Synonyms: ARAT; GS1999FULL; HMFN1045
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC104559 sequence for NM_032564 edited (data generated by NextGen Sequencing); ATGAAGACCCTCATAGCCGCCTACTCCGGGGTCCTGCGCGGCGAGCGTCAGGCCGAGGCT; GACCGGAGCCAGCGCTCTCACGGAGGACCTGCGCTGTCGCGCGAGGGGTCTGGGAGATGG; GGCACTGGATCCAGCATCCTCTCCGCCCTCCAGGACCTCTTCTCTGTCACCTGGCTCAAT; AGGTCCAAGGTGGAAAAGCAGCTACAGGTCATCTCAGTGCTCCAGTGGGTCCTGTCCTTC; CTTGTACTGGGAGTGGCCTGCAGTGCCATCCTCATGTACATATTCTGCACTGATTGCTGG; CTCATCGCTGTGCTCTACTTCACTTGGCTGGTGTTTGACTGGAACACACCCAAGAAAGGT; GGCAGGAGGTCACAGTGGGTCCGAAACTGGGCTGTGTGGCGCTACTTTCGAGACTACTTT; CCCATCCAGCTGGTGAAGACACACAACCTGCTGACCACCAGGAACTATATCTTTGGATAC; CACCCCCATGGTATCATGGGCCTGGGTGCCTTCTGCAACTTCAGCACAGAGGCCACAGAA; GTGAGCAAGAAGTTCCCAGGCATACGGCCTTACCTGGCTACACTGGCAGGCAACTTCCGA; ATGCCTGTGTTGAGGGAGTACCTGATGTCTGGAGGTATCTGCCCTGTCAGCCGGGACACC; ATAGACTATTTGCTTTCAAAGAATGGGAGTGGCAATGCTATCATCATCGTGGTCGGGGGT; GCGGCTGAGTCTCTGAGCTCCATGCCTGGCAAGAATGCAGTCACCCTGCGGAACCGCAAG; GGCTTTGTGAAACTGGCCCTGCGTCATGGAGCTGACCTGGTTCCCATCTACTCCTTTGGA; GAGAATGAAGTGTACAAGCAGGTGATCTTCGAGGAGGGCTCCTGGGGCCGATGGGTCCAG; AAGAAGTTCCAGAAATACATTGGTTTCGCCCCATGCATCTTCCATGGTCGAGGCCTCTTC; TCCTCCGACACCTGGGGGCTGGTGCCCTACTCCAAGCCCATCACCACTGTTGTGGGAGAG; CCCATCACCATCCCCAAGCTGGAGCACCCAACCCAGCAAGACATCGACCTGTACCACACC; ATGTACATGGAGGCCCTGGTGAAGCTCTTCGACAAGCACAAGACCAAGTTCGGCCTCCCG; GAGACTGAGGTCCTGGAGGTGAACTGA; ; Clone variation with respect to NM_032564.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032564 unedited; TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGTGACTGGGCG; GGCTTCAGCCATGAAGACCCTCATAGCCGCCTACTCCGGGGTCCTGCGCGGCGAGCGTCA; GGCCGAGGCTGACCGGAGCCAGCGCTCTCACGGAGGACCTGCGCTGTCGCGCGAGGGGTC; TGGGAGATGGGGCACTGGATCCAGCATCCTCTCCGCCCTCCAGGACCTCTTCTCTGTCAC; CTGGCTCAATAGGTCCAAGGTGGAAAAGCAGCTACAGGTCATCTCAGTGCTCCAGTGGGT; CCTGTCCTTCCTTGTACTGGGAGTGGCCTGCAGTGCCATCCTCATGTACATATTCTGCAC; TGATTGCTGGCTCATCGCTGTGCTCTACTTCACTTGGCTGGTGTTTGACTGGAACACACC; CAAGAAAGGTGGCAGGAGGTCACAGTGGGTCCGAAACTGGGCTGTGTGGCGCTACTTTCG; AGACTACTTTCCCATCCAGCTGGTGAAGACACACAACCTGCTGACCACCAGGAACTATAT; CTTTGGATACCACCCCCATGGTATCATGGGCCTGGGTGCCTTCTGCAACTTCAGCACAGA; GGCCACAGAAGTGAGCAAGAAGTTCCCAGGCATACGGGCTTACCTGGCTACACTGGCAGG; CAACTTCCGAATGCCTGTGTTGAGGGAGTACCTGATGTCTGGNAGTATCTGCCCTGTCAG; CCGGGACACATAGACTATTTGCTTTCAAAGAATGGGAGGGGCAATGCTATCATCATCGGG; GTCGGGGGGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032564 unedited; NNNAAAANTTTTCTTGNACCGCGCCGCAATCTANGATCGGTTTTTTTTTTTTTTTTTTGG; TTTAGCAAAATTGTTATAATTTCTTTTAAATAACCCACAGACACCCATGACACTTCCAAA; TTTACAGAGCAAAAAAGTGATTTGCAGCTGGTTCCTCCAGGGAATTGGCCCCGAAGGCTG; GCTCAGTTCACCTCCAGGACCTCAGTCTCCGGGAGGCCGAACTTGGTCTTGTGCTTGTCG; AAGAGCTTCACCAGGGCCTCCATGTACATGGTGTGGTACAGGTCGATGTCTTGCTGGGTT; GGGTGCTCCAGCTTGGGGATGGTGATGGGCTCTCCCACAACAGTGGTGATGGGCTTGGAG; TAGGGCACCAGCCCCCAGGTGTCNGAGGAGAAGAAGCCTCGACCATGGAGATGCATGGNG; CGAAACCATGTATTTCTGGACTTCTTTTGACCCATCGCCCCCAGAGCCTCCTCGAAGATC; ACTGCTTGACACTCCATTCTCTCAAGGAGTNATGGGACCAGGTCAGCTCATGACCAGGCC; AGTTTACAAAGCCTTGCGTCCCCAGGTGACTGCATCTTGCAGGCTGGACTAAAGACTTAC; CGGCCCCCACCACATATGATGCATGCCCTCCATTCTTGAAGCAAATATCATGGGTCCGGG; TTGCAGCAAATCCTCAAACTTGGTCTCCCTAAACAGCTTTTGAAATTCCTCCATGTAACC; AGTAGGGCCTTCCCTGGAACTTTTGCCATTTTGGGCCCTTGGCTAAATGCCAAAGCCCCA; GCCCTTATCCCTGGGGGGGATCCAAAATATTCCCTGGGGCAAAGTTTGGTTTTTCCCACT; GGGGGAAAGTTCCTAAAATCGCCCCAGCCATTTGGCCCCTGGCCCCCCCCCCTTTTGGGG; GTCACCACACACCCAAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_032564
• Insert Size: 1380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032564.2, NP_115953.2
• RefSeq Size: 2439 bp
• RefSeq ORF: 1167 bp
• Locus ID: 84649
• UniProt ID: Q96PD7
• Cytogenetics: 11q13.5
• Domains: DAGAT
• Protein Families: Transmembrane
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways, Retinol metabolism
• Summary: This gene encodes one of two enzymes which catalyzes the final reaction in the synthesis of triglycerides in which diacylglycerol is covalently bound to long chain fatty acyl-CoAs. The encoded protein catalyzes this reaction at low concentrations of magnesium chloride while the other enzyme has high activity at high concentrations of magnesium chloride. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).