p35 (CDK5R1) (BC035448) Human Untagged Clone

CAT#: SC103532

(untagged)-Homo sapiens, clone IMAGE:4939043

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: p35
• Synonyms: CDK5P35; CDK5R; NCK5A; p23; p25; p35; p35nck5a
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC035448, the custom clone sequence may differ by one or more nucleotides
  
  
  GCACCTGTCCCCCACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCGCTGCTTCTGCAGGGCTGG
  CAGGACCAGGGCTTCATCACGCCGGCCAACGTGGTCTTCCTCTACATGCTCTGCAGGGATGTTATCTCCT
  CCGAGGTGGGCTCGGATCACGAGCTCCAGGCCGTCCTGCTGACATGCCTGTACCTCTCCTACTCCTACAT
  GGGCAACGAGATCTCCTACCCGCTCAAGCCCTTCCTGGTGGAGAGCTGCAAGGAGGCCTTTTGGGACCGT
  TGCCTCTCTGTCATCAACCTCATGAGCTCAAAGATGCTGCAGATAAATGCCGACCCACACTACTTCACAC
  AGGTCTTCTCCGACCTGAAGAACGAGAGCGGCCAGGAGGACAAGAAGCGGCTCCTCCTAGGCCTGGATCG
  GTGAGCACTGTAGCCTGCGTCATGGCTCAAGGATTCAATGCATTTTTAAGAATTTATTATTAAATCAGTT
  TTGTGTACAGTATGTGTCTAGCAAAGCCACCAAGGGCCTCACCTTTCCCACAGTCTCTCCCTGGGGTTTT
  TTTCATCCCTGCCAAGAACTCTGGGCACTTTTGAACTCACGAGCCTTGCGCAAAACCCAGAAGATGTATT
  CAGAGCCACCCAGGCCACTGACCTCCCACTTTGGGGAACTCAAAGGACTGACCTGCCCCTGCCGCCTGTG
  CCCTTGCTGGGTCCAGGGTAGGCAAGGCTGCCGGCTGCACCCTGTATGGAGCTAGAGAGGGGCCTCTGGC
  TGCCTGGCCCAGGGAGGAATTGGGGTTTCTGGTTGGAGGCCCTTTTCTGGCTCCTGTGTGGAGTTATTCA
  CTCTCCCAGAGGCTCCTGGAGCCAGCCACCCTAACTGAGCTGCCAGTGGGGTCGTGAGGCAAGATCCCCG
  CCACCCGGGGACATCTTCAATCTAGGCGAGGCGAAGCTGAGCGGGTCTAGTGGAAAGATTGTGTCTGGTC
  GTTTGACCACACACCGCCCTGATTTGCTGTTTTCTTTTTTTAGGGAGAAGGGCTTTTCTTTAGTGGAGAA
  ATGGAACTCGCCCCCCTACCCCCTTGTCTGCTGCTCCCAGCCACGTTGGTGGTATTGGCCGATGAGCTGG
  TTTGACTCATTAATTTCTCTCAATTTGGGTCCCAGCTAAAGAGGTGGGGTGAAGCTGGGGACAGCTTTCC
  TGGGTGAGTTTTTCTTTTGAATAATGTAGTGCAGTCACCCTGTGGCAAATGCCAGGACAGCTGCAGGTGC
  CCATTAGCAACGCCTGCCTTCTGGGCAAGGTGAGGGATGCCATACCTTGAGACCAAGCCGGTGCCCACCT
  AAGGGCTAAGGCCTCTTGCTGGTGCACATGACATTTGTCCTGCAGAGCTGGGGGGAAGGCGATGTGGTGA
  CCCCACCCCCACCCGTTAATTTAAAGCTGTTTCTAAACAGTTGAGTTTCTTCTAAAGAGGAAGCCTTGCC
  CAGCAAGGACCAGTGAGACAGCCGGATCTTGGAGACAATTACAAGACGGGGAGTGAGACTCCTGATTGCT
  CTGGAAGCCTGCTGATTTCTACAACTGATCATTTGCAGCTGCTGGTTTTGGTTTCCACCTTACCCTACTG
  GCTGTAAAAACACAAATGTGTACTTTATTGATTTTCTTTCTAATTCTCCCGCATTGGTGGCTTGGGACTT
  GGGAGAGGGAGCAAGCCTTCCTCCATGGCCCATCACTCGGCTGTGGAGAACAAAGACCAATGTGAAGACA
  CTACAGAGGATTCTGTCTTCCAGGCCCAGTCCACTGGGGAGTGCTGGAATGGGGACCTGGGGTGGGGAGG
  CAGAGGGTCACTTTACATAGGATTAAGTTCGAGGTGGCTACCGATTTCAGCACATGCACTACTGAAATTT
  ACACAAAAAGAAAGCTGTGAAATTGAAGTCCCAATTTAAGAGTCCTGAGGCAGAACCTGGTGGCTGGAGG
  CATTCCCAGAGGTGGGGAAGAGAGCCTGCCCGGCCGGAGAACATCTGCCTTGCTGCACCTGAGGCCCAGC
  AGAGCCGTTCCTGGGACTGTCAGATAATCGGTGCAGCGGTGGAAGGAGCCTGCGGCTGCTGGCACAGACT
  TCACACAGCACCTCCTCTCTGCTGGGTTTCCACACAGCCTGTCTTCAGATCCTGCTGCCGCGTGCGACCA
  GAGGTGGGAGGCCCCTGGTGGCATGGAAGAGGGAGGGTCAGTGCCAAGTCTCAGGAGGAGGGCGCATGTG
  TGTATCACCCTCAGCTGGCGGAACCTGGCTGCGAACTGTGCAGTTACGTTGCATCCACAGGATTCCAGTT
  GCGTGTCTGTTTCCTTCTCTTTCTCCGTATTTATTTTTTTATTCTTCGGAGGAGGTGGACATTTCGGAAG
  TGGTGGGGACTAAGGGAAGAACTCTCTAGTTCCCTCAGTGTGAAGCCTGTCGTGTTCTCTCCCCTTGCAC
  TGGTCATCAGTATTGTGTAAAGGAACAACTGATATACTTGAGTGTGCAAGCAAAGAACCCATTTGCCATG
  CTGCTATGAAGACTACTTTTAGATCAACAATAAAAAAAAACCTACAAAAAAACCTTTATTCTTTAATTGT
  TGCTTTTACGGTGATATTGTGCATGCAAACCAGGAGCATTTTGTGTCTTAAGAAAAATAATCTTAGAACA
  GATGGCTGTGAAAATTACACCCATGCACAGAACAAGCCACAGGAATAATAGTTCAGGATTTGGTTTTTCT
  CTTTTTCTTGTAAACCTGGAGGGTTGATATATTCTTTCCATGCAGTTATTAGAACTTAGTTTTGTTCCAA
  CAGTTAAACTTGCAATGAAAAGAAAATGTGCCATTTTTTTCACTCAGAATTATTCATAGCTGTATATTTG
  AAACTGCTAATTACACACGTGTGATGTATGTTGGTTTTTTAGTGCAATTTCTTCTGTAGCTATTCTTTGA
  CCAAACTGTGGGTATTGTTAATATTAATTTATATTTGTCTCATTTTGTATGTATGTGTAGTGTGTTTGTG
  AGTATGTGTGGTTTATAATCTGACAAAGTCATGAAGCTCAGTTTGGCTGTAATTTAATTCCCCTTCCCTT
  ATTTTTATTTATTTTTGTACTGTGCTGATTCAATAAAATGCACTGACCATCCATAAAAAAAAAAAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC035448 unedited
  GGTGCACCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGC
  CTGCACCCCCGGCCAGCCAGCTCTCGGGTTCCCAGACCGGGGGCTCCTCCTCAGTCAAGA
  AAGCCCCTCACCCTGCCGTCACCTCCGCAGGGACGCCCAAACGGGTCATCGTCCAGGCGT
  CCACCAGTGAGCTGCTTCGCTGCCTGGGTGAGTTTCTCTGCCGCCGGTGCTACCGCCTGA
  AGCACCTGTCCCCCACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCGCTGCTTC
  TGCAGGGCTGGCAGGACCAGGGCTTCATCACGCCGGCCAACGTGGTCTTCCTCTACATGC
  TCTGCAGGGATGTTATCTCCTCCGAGGTGGGCTCGGATCACGAGCTCCAGGCCGTCCTGC
  TGACATGCCTGTACCTCTCCTACTCCTACATGGGCAACGAGATCTCCTACCCGCTCAAGC
  CCTTCCTGGTGGAGAGCTGCAAGGAGGCCTTTTGGGACCGTTGCCTCTCTGTCATCAACC
  TCATGAGCTCAAAGATGCTGCAGATAAATGCCGACCCACACTACTTCACACAGGTCTTCT
  CCGACCTGAAGAACGAGAGCGGCCAGGAGGACAAGAAGCGGCTCCTCCTANGCCTGGATC
  GGTGAGCACTGTAGCCTGCGTCATGGCTCAAGGATTCAATGCATNTTTAAGAATNTATTA
  TTAAATCAGTTTTGTGTACAGTATGTGTCTAGCAAAGCCACCAAAGGCCTCACCTTNTCC
  ACAGTCTCTCCCTGGGGTTTTTTTCATTCCTGCCAAGAACTCTGGGCACTTTTGAACTCA
  CGAGCCTTGCGCAAAAACCAGAAGATGTATNCAGAAGCCACCAGGCCACTGACCTCCCAC
  TTTGGGGACTCAAAGACCTGACTGCC
  
• Restriction Sites: NotI-NotI
• ACCN: BC035448
• Insert Size: 3800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC035448.1
• RefSeq Size: 3149 bp
• RefSeq ORF: 3149 bp
• Locus ID: 8851
• Cytogenetics: 17q11.2
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease
• Gene Summary: The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008]