WNT9A (NM_003395) Human Untagged Clone

CAT#: SC102858

WNT9A (untagged)-Human wingless-type MMTV integration site family, member 9A (WNT9A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WNT9A
• Synonyms: WNT14
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC102858 sequence for NM_003395 edited (data generated by NextGen Sequencing)
  ATGCTGGATGGGTCCCCGCTGGCGCGCTGGCTGGCCGCGGCCTTCGGGCTGACGCTGCTG
  CTCGCCGCGCTGCGCCCTTCGGCCGCCTACTTCGGGCTGACGGGCAGCGAGCCCCTGACC
  ATCCTCCCGCTGACCCTGGAGCCAGAGGCGGCTGCCCAGGCGCACTACAAGGCCTGCGAC
  CGGCTGAAGCTGGAGCGGAAGCAGCGGCGCATGTGCCGCCGGGACCCGGGCGTGGCAGAG
  ACGCTGGTGGAGGCCGTGAGCATGAGTGCGCTCGAGTGCCAGTTCCAGTTCCGCTTTGAG
  CGCTGGAACTGCACGCTGGAGGGCCGCTACCGGGCCAGCCTGCTCAAGCGAGGCTTCAAG
  GAGACTGCCTTCCTCTATGCCATCTCCTCGGCTGGCCTGACGCACGCACTGGCCAAGGCG
  TGCAGCGCGGGCCGCATGGAGCGCTGTACCTGCGATGAGGCACCCGACCTGGAGAACCGT
  GAGGCCTGGCAGTGGGGGGGCTGCGGAGACAACCTTAAGTACAGCAGCAAGTTCGTCAAG
  GAATTCCTGGGCAGACGGTCAAGCAAGGATCTGCGAGCCCGTGTGGACTTCCACAACAAC
  CTCGTGGGTGTGAAGGTGATCAAGGCTGGGGTGGAGACCACCTGCAAGTGCCACGGCGTG
  TCAGGCTCATGCACGGTGCGGACCTGCTGGCGGCAGTTGGCGCCTTTCCATGAGGTGGGC
  AAGCATCTGAAGCACAAGTATGAGACGGCACTCAAGGTGGGCAGCACCACCAATGAAGCT
  GCCGGCGAGGCAGGTGCCATCTCCCCACCACGGGGCCGTGCCTCGGGGGCAGGTGGCAGC
  GACCCGCTGCCCCGCACTCCAGAGCTGGTGCACCTGGATGACTCGCCTAGCTTCTGCCTG
  GCTGGCCGCTTCTCCCCGGGCACCGCTGGCCGTAGGTGCCACCGTGAGAAGAACTGCGAG
  AGCATCTGCTGTGGCCGCGGCCATAACACACAGAGCCGGGTGGTGACAAGGCCCTGCCAG
  TGCCAGGTGCGTTGGTGCTGCTATGTGGAGTGCAGGCAGTGCACGCAGCGTGAGGAGGTC
  TACACCTGCAAGGGCTGA
  
  Clone variation with respect to NM_003395.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003395 unedited
  TATTGTATACGACTCCTATAGGCGGCCGCGACATTCGCACGAGGCGATGGTCGGCCGCCG
  AGGCGCGGCAAGATGCTGGATGGGTCCCCGCTGGCGCGCTGGCTGGCCGCGGCCTTCGGG
  CTGACGCTGCTGCTCGCCGCGCTGCGCCCTTCGGCCGCCTACTTCGGGCTGACGGGCAGC
  GAGCCCCTGACCATCCTCCCGCTGACCCTGGAGCCAGAGGCGGCCGCCCAGGCGCACTAC
  AAGGCCTGCGACCGGCTGAAGCTGGAGCGGAAGCAGCGGCGCATGTGCCGCCGGGACCCG
  GGCGTGGCAGAGACGCTGGTGGAGGCCGTGAGCATGAGTGCGCTCGAGTGCCAGTTCCAG
  TTCCGCTTTGAGCGCTGGAACTGCACGCTGGAGGGCCGCTACCGGGCCAGCCTGCTCAAG
  CGAGGCTTCAAGGAGACTGCCTTCCTCTATGCCATCTCCNCGGCTGGCCTGACGCACGCA
  CTGGCCAAGGCGTGCANCGCGGGCCGCATGGAGCGCTGTACCTGCGATGAGGCACCCGAC
  CTGGAGAACCGTGAGGCCTGGCAGTGGNGGGGGCTGCGGAGACAACCTTAAGTACAGCAG
  CAAGTTCGTCAAGGAATTCCTGGGCAGACGGTCAAGCAAGGATCTGCGAGCCCGTGGTGG
  ACTTCCCAACAACCTCGTGGGTGTGGAAAGTGATCAAGCTGGGGGGGGGAGACCCACCTG
  CAGTGCCAGGCGGTGTCAGGCTCATGCACGGGGGCGAACCTGCTGGCGGCAGTTGGGCGC
  TTTCCATGGAGGTGGGCAAGCATTTTGAACCCAAGTATGAAAACGGACTTAAGGTGGGGC
  AGCACCCACAATGAAGCTGCGGCCGAGGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003395 unedited
  CTCGCGGTTCGCTTTCTATTGCNCGGTTTTTTTTTTTTTTTTTTATAATTCAAAAACCCC
  CATTTAATGTTTCCATTTGTTTCTGGTTCTGTGACACAAAGCTGTTAAATAAATACATTC
  TCCACAGCCCCTTTGGGACGGGCCACACAGATGAGGCAGAGAAATCATTGTATGCACGGA
  GACTCGGAGCACAGACAGGGCCCCCCCCACCCACCCATTTTGTTTATTTTCTCATGGGGG
  ACCAGGCCCCTCCAGGCCGCCCCTGAAGCCCCACAGGGGGTATTTCATCTATTGTTTAAG
  GAAAGAAAAACCACCCGTACCCACCTCCCGCCGCGCCCCCCCCAGGACTATTTATACACA
  CTCTTCGTGTTTTTTTTTTATGTGCATGATTATATTGTCGCGCCCCTCGACCTCCCCCCC
  TCCCGGCTTCCCCGCTTTTTATATTTTATTTTTCCGTCGAATCCGATCCCCCCCCCGCCC
  CCCCCCTACACGTAACCTTCTCCCGTCCCTTCGTCTTTATCACCGTCCCCCCGCCCCTTC
  CTTCCCGCCCCGTTTTTTTTTCCCTCCTTTCTTTTTTTCTTTCCCCCCACTCTCGTTCGC
  CGTTCCCCTGCAGTCACGCTTCGCTCCTCGCCTCTCCTTTATCCGCTTTTCCGCCACCAT
  GTTCTTCCTCCGCCGCCTCCACGCCCTTGTCCTGGATGCGCCGTTTTCTTCTCGCGCCCT
  TATTCCGCCGCCTGTCCTCCGTTCCCCATGTTTTCTTCTTTTGCCCCACTTTTTTTGGTA
  ATGGATTTTCCGACCCTCGCCGCCCCTGCCCGCCCCCTCTTCCTTCTCCCCCCCCCCCTC
  CCTCTCCCGCGTGCTTAATAATCCCCGCTCTTCTATTCCCGCCCCTTCTGCCACCTTCTT
  CGCCACCGACCCGACGGCCTTTATTCCCCTTATTCATGTGCCCCGCCGCGTCGTTGCAGC
  GTTCGTCGGCGCGCGCGGGNATAANAAGGCCGGCGTAGTATCTTATTTCCGCCATCCGCC
  GCTCCTATCCGCTTATTCTCCGCGCCGCCGCGTGTTTTTCGCTCCGCGTCTTCTCTCCTT
  TCCTATCCATCTGGCCGTCTTGTTTCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003395
• Insert Size: 3780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003395.1, NP_003386.1
• RefSeq Size: 1631 bp
• RefSeq ORF: 1098 bp
• Locus ID: 7483
• UniProt ID: O14904
• Cytogenetics: 1q42.13
• Domains: wnt
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway
• Gene Summary: The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is expressed in gastric cancer cell lines. The protein encoded by this gene shows 75% amino acid identity to chicken Wnt14, which has been shown to play a central role in initiating synovial joint formation in the chick limb. This gene is clustered with another family member, WNT3A, in the chromosome 1q42 region. [provided by RefSeq, Jul 2008]