CXCR5 (NM_032966) Human Untagged Clone

CAT#: SC102158

CXCR5 (untagged)-Human chemokine (C-X-C motif) receptor 5 (CXCR5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CXCR5
• Synonyms: BLR1; CD185; MDR15
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032966 edited
  CCTCTCAACATAAGACAGTGACCAGTCTGGTGACTCACAGCCGGCACAGCCATGAACTAC
  CCGCTAACGCTGGAAATGGACCTCGAGAACCTGGAGGACCTGTTCTGGGAACTGGACAGA
  TTGGACAACTATAACGACACCTCCCTGGTGGAAAATCATCTCTGCCCTGCCACAGAGGGG
  CCCCTCATGGCCTCCTTCAAGGCCGTGTTCGTGCCCGTGGCCTACAGCCTCATCTTCCTC
  CTGGGCGTGATCGGCAACGTCCTGGTGCTGGTGATCCTGGAGCGGCACCGGCAGACACGC
  AGTTCCACGGAGACCTTCCTGTTCCACCTGGCCGTGGCCGACCTCCTGCTGGTCTTCATC
  TTGCCCTTTGCCGTGGCCGAGGGCTCTGTGGGCTGGGTCCTGGGGACCTTCCTCTGCAAA
  ACTGTGATTGCCCTGCACAAAGTCAACTTCTACTGCAGCAGCCTGCTCCTGGCCTGCATC
  GCCGTGGACCGCTACCTGGCCATTGTCCACGCCGTCCATGCCTACCGCCACCGCCGCCTC
  CTCTCCATCCACATCACCTGTGGGACCATCTGGCTGGTGGGCTTCCTCCTTGCCTTGCCA
  GAGATTCTCTTCGCCAAAGTCAGCCAAGGCCATCACAACAACTCCCTGCCACGTTGCACC
  TTCTCCCAAGAGAACCAAGCAGAAACGCATGCCTGGTTCACCTCCCGATTCCTCTACCAT
  GTGGCGGGATTCCTGCTGCCCATGCTGGTGATGGGCTGGTGCTACGTGGGGGTAGTGCAC
  AGGTTGCGCCAGGCCCAGCGGCGCCCTCAGCGGCAGAAGGCAGTCAGGGTGGCCATCCTG
  GTGACAAGCATCTTCTTCCTCTGCTGGTCACCCTACCACATCGTCATCTTCCTGGACACC
  CTGGCGAGGCTGAAGGCCGTGGACAATACCTGCAAGCTGAATGGCTCTCTCCCCGTGGCC
  ATCACCATGTGTGAGTTCCTGGGCCTGGCCCACTGCTGCCTCAACCCCATGCTCTACACT
  TTCGCCGGCGTGAAGTTCCGCAGTGACCTGTCGCGGCTCCTGACGAAGCTGGGCTGTACC
  GGCCCTGCCTCCCTGTGCCAGCTCTTCCCTAGCTGGCGCAGGAGCAGTCTCTCTGAGTCA
  GAGAATGCCACCTCTCTCACCACGTTCTAGGTCCCAGTGTCCCCTTTTATTGCTGCTTTT
  CCTTGGGGCAGGCAGTGATGCTGGATGCTCCTTCCAACAGGAGCTGGGATCCTAAGGGCT
  CACCGTGGCTAAGAGTGTCCTAGGAGTATCCTCATTTGGGGTAGCTAGAGGAACCAACCC
  CCATTTCTAGAACATCCCTGCCAGCTCTTCTGCCGGCCCTGGGGCTAGGCTGGAGCCCAG
  GGAGCGGAAAGCAGCTCAAAGGCACAGTGAAGGCTGTCCTTACCCATCTGCACCCCCCTG
  GGCTGAGAGAACCTCACGCACCTCCCATCCTAATCATCCAATGCTCAAGAAACAACTTCT
  ACTTCTGCCCTTGCCAACGGAGAGCGCCTGCCCCTCCCAGAACACACTCCATCAGCTTAG
  GGGCTGCTGACCTCCACAGCTTCCCCTCTCTCCTCCTGCCCACCTGTCAAACAAAGCCAG
  AAGCTGAGCACCAGGGGATGAGTGGAGGTTAAGGCTGAGGAAAGGCCAGCTGGCAGCAGA
  GTGTGGCCTTCGGACAACTCAGTCCCTAAAAACACAGACATTCTGCCAGGCCCCCAAGCC
  TGCAGTCATCTTGACCAAGCAGGAAGCTCAGACTGGTTGAGTTCAGGTAGCTGCCCCTGG
  CTCTGACCGAAACAGCGCTGGGTCCACCCCATGTCACCGGATCCTGGGTGGTCTGCAGGC
  AGGGCTGACTCTAGGTGCCCTTGGAGGCCAGCCAGTGACCTGAGGAAGCGTGAAGGCCGA
  GAAGCAAGAAAGAAACCCGACAGAGGGAAGAAAAGAGCTTTCTTCCCGAACCCCAAGGAG
  GGAGATGGATCAATCAAACCCGGCGGTCCCCTCCGCCAGGCGAGATGGGGTGGGGTGGAG
  AACTCCTAGGGTGGCTGGGTCCAGGGGATGGGAGGTTGTGGGCATTGATGGGGAAGGAGG
  CTGGCTTGTCCCCTCCTCACTCCCTTCCCATAAGCTATAGACCCGAGGAAACTCAGAGTC
  GGAACGGAGAAAGGTGGACTGGAAGGGGCCCGTGGGAGTCATCTCAACCATCCCCTCCGT
  GGCATCACCTTAGGCAGGGAAGTGTAAGAAACACACTGAGGCAGGGAAGTCCCCAGGCCC
  CAGGAAGCCGTGCCCTGCCCCCGTGAGGATGTCACTCAGATGGAACCGCAGGAAGCTGCT
  CCGTGCTTGTTTGCTCACCTGGGGTGTGGGAGGCCCGTCCGGCAGTTCTGGGTGCTCCCT
  ACCACCTCCCCAGCCTTTGATCAGGTGGGGAGTCAGGGACCCCTGCCCTTGTCCCACTCA
  AGCCAAGCAGCCAAGCTCCTTGGGAGGCCCCACTGGGGAAATAACAGCTGTGGCTCACGT
  GAGAGTGTCTTCACGGCAGGACAACGAGGAAGCCCTAAGACGTCCCTTTTTTCTCTGAGT
  ATCTCCTCGCAAGCTGGGTAATCGATGGGGGAGTCTGAAGCAGATGCAAAGAGGCAAGAG
  GCTGGATTTTGAATTTTCTTTTTAATAAAAAGGCACCTATAAAACAGGTCAATACAGTAC
  AGGCAGCACAGAGACCCCCGGAACAAGCCTAAAAATTGTTTCAAAATAAAAACCAAGAAG
  ATGTCTTC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032966 unedited
  TCGGGATTTGNTATACGACTCACTATAGGGCGGCACGCGAATTCGCACCAGGAAGCCTCT
  CAACATAAGACAGTGACCAGTCTGGTGACTCACATCCGGCACAGCCATGAACTACCCGCT
  AACGCTGGAAATGGACCTCGAGAACCTGGAGGACCTGTTCTGGGAACTGGACAGATTGGA
  CAACTATAACGACACCTCCCTGGTGGAAAATCATCTCTGCCCTGCCACAGAGGGGCCCCT
  CATGGCCTCCTTCAAGGCCGTGTTCGTGCCCGTGGCCTACAGCCTCATCTTCCTCCTGGG
  CGTGATCGGCAACGTCCTGGTGCTGGTGATCCTGGAGCGGCACCGGCAGACACGCAGTTC
  CACGGAGACCTTCCTGTTCCACCTGGCCGTGGCCGACCTCCTGCTGGTCTTCATCTTGCC
  CTTTGCCGTGGCCGATGGCTCTGTGGGCTGGGTCCTGGGGACCTTCCTCTGCAAAACTGT
  GATTGCCCTGCACAAAGTCAACTTCTACTGCAGCAGCCTGCTCCTGGCCTGCATCGCCGT
  GGACCGCTACCTGGCCATTGTCCACGCCGTCCATGCCTACCGCCACCGNCGNCTCCTCTC
  CATCCACATCACCTGTGGGACCATCTGGCTGGTGGGCTTCCTCCTTGCCTTGCCAGAGAT
  TCTCTTCGCCAAAGTCAGCCAAAGCCATCACAACAACTCCCTGCCACGTTGCACCTTTCT
  CCAAGATATCCAAGCAGAAACGCATGCCTGGNTCACCTTCCGATTCCTCTACCATGTGGC
  GGGATTCCTGCCTGCCATGCTGGTGATGGGCTGGTGCTACCTGGGATTAAAGCACAGGGT
  GCGCCAAGTCCATCGGCGCCCTCATCGGAGAAAGCAGTCAAGGTGGCATTCTGTAGAAAA
  CATTTCTTCTTTGCTGTTCACCTACACCATTGTATTTCCTGGAACCTGCGAAGCTGTAGC
  CCGGGACAATCCTGCAGCTGAATGCCTTTCCCCGG
  
• Restriction Sites: Please inquire
• ACCN: NM_032966
• Insert Size: 2800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032966.1, NP_116743.1
• RefSeq Size: 2893 bp
• RefSeq ORF: 984 bp
• Locus ID: 643
• UniProt ID: P32302
• Cytogenetics: 11q23.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction
• Gene Summary: This gene encodes a multi-pass membrane protein that belongs to the CXC chemokine receptor family. It is expressed in mature B-cells and Burkitt's lymphoma. This cytokine receptor binds to B-lymphocyte chemoattractant (BLC), and is involved in B-cell migration into B-cell follicles of spleen and Peyer patches. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (2) differs at the 5' end compared to variant 1. This results in translation initiation from an in-frame downstream AUG, and an isoform (2) with a shorter N-terminus compared to isoform 1.