Lamin A (LMNA) (NM_170707) Human Untagged Clone

CAT#: SC101048

LMNA (untagged)-Human lamin A/C (LMNA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Lamin A
• Synonyms: CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; PRO1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101048 sequence for NM_170707 edited (data generated by NextGen Sequencing)
  ATGGAGACCCCGTCCCAGCGGCGCGCCACCCGCAGCGGGGCGCAGGCCAGCTCCACTCCG
  CTGTCGCCCACCCGCATCACCCGGCTGCAGGAGAAGGAGGACCTGCAGGAGCTCAATGAT
  CGCTTGGCGGTCTACATCGACCGTGTGCGCTCGCTGGAAACGGAGAACGCAGGGCTGCGC
  CTTCGCATCACCGAGTCTGAAGAGGTGGTCAGCCGCGAGGTGTCCGGCATCAAGGCCGCC
  TACGAGGCCGAGCTCGGGGATGCCCGCAAGACCCTTGACTCAGTAGCCAAGGAGCGCGCC
  CGCCTGCAGCTGGAGCTGAGCAAAGTGCGTGAGGAGTTTAAGGAGCTGAAAGCGCGCAAT
  ACCAAGAAGGAGGGTGACCTGATAGCTGCTCAGGCTCGGCTGAAGGACCTGGAGGCTCTG
  CTGAACTCCAAGGAGGCCGCACTGAGCACTGCTCTCAGTGAGAAGCGCACGCTGGAGGGC
  GAGCTGCATGATCTGCGGGGCCAGGTGGCCAAGCTTGAGGCAGCCCTAGGTGAGGCCAAG
  AAGCAACTTCAGGATGAGATGCTGCGGCGGGTGGATGCTGAGAACAGGCTGCAGACCATG
  AAGGAGGAACTGGACTTCCAGAAGAACATCTACAGTGAGGAGCTGCGTGAGACCAAGCGC
  CGTCATGAGACCCGACTGGTGGAGATTGACAATGGGAAGCAGCGTGAGTTTGAGAGCCGG
  CTGGCGGATGCGCTGCAGGAACTGCGGGCCCAGCATGAGGACCAGGTGGAGCAGTATAAG
  AAGGAGCTGGAGAAGACTTATTCTGCCAAGCTGGACAATGCCAGGCAGTCTGCTGAGAGG
  AACAGCAACCTGGTGGGGGCCGCCCACGAGGAGCTGCAGCAGTCGCGCATCCGCATCGAC
  AGCCTCTCTGCCCAGCTCAGCCAGCTCCAGAAGCAGCTGGCAGCCAAGGAGGCGAAGCTT
  CGAGACCTGGAGGACTCACTGGCCCGTGAGCGGGACACCAGCCGGCGGCTGCTGGCGGAA
  AAGGAGCGGGAGATGGCCGAGATGCGGGCAAGGATGCAGCAGCAGCTGGACGAGTACCAG
  GAGCTTCTGGACATCAAGCTGGCCCTGGACATGGAGATCCACGCCTACCGCAAGCTCTTG
  GAGGGCGAGGAGGAGAGGCTACGCCTGTCCCCCAGCCCTACCTCGCAGCGCAGCCGTGGC
  CGTGCTTCCTCTCACTCATCCCAGACACAGGGTGGGGGCAGCGTCACCAAAAAGCGCAAA
  CTGGAGTCCACTGAGAGCCGCAGCAGCTTCTCACAGCACGCACGCACTAGCGGGCGCGTG
  GCCGTGGAGGAGGTGGACGAGGAGGGCAAGTTTGTCCGGCTGCGCAACAAGTCCAATGAG
  GACCAGTCCATGGGCAATTGGCAGATCAAGCGCCAGAATGGAGATGATCCCTTGCTGACT
  TACCGGTTCCCACCAAAGTTCACCCTGAAGGCTGGGCAGGTGGTGACGATCTGGGCTGCA
  GGAGCTGGGGCCACCCACAGCCCCCCTACCGACCTGGTGTGGAAGGCACAGAACACCTGG
  GGCTGCGGGAACAGCCTGCGTACGGCTCTCATCAACTCCACTGGGGAAGAAGTGGCCATG
  CGCAAGCTGGTGCGCTCAGTGACTGTGGTTGAGGACGACGAGGATGAGGATGGAGATGAC
  CTGCTCCATCACCACCACGGCTCCCACTGCAGCAGCTCGGGGGACCCCGCTGAGTACAAC
  CTGCGCTCGCGCACCGTGCTGTGCGGGACCTGCGGGCAGCCTGCCGACAAGGCATCTGCC
  AGCGGCTCAGGAGCCCAGGTGGGCGGACCCATCTCCTCTGGCTCTTCTGCCTCCAGTGTC
  ACGGTCACTCGCAGCTACCGCAGTGTGGGGGGCAGTGGGGGTGGCAGCTTCGGGGACAAT
  CTGGTCACCCGCTCCTACCTCCTGGGCAACTCCAGCCCCCGAACCCAGAGCCCCCAGAAC
  TGCAGCATCATGTAA
  
  Clone variation with respect to NM_170707.2
  861 t=>c;1338 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_170707 unedited
  GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCACCTACA
  CCAGCCAACCCANATCCCGAGGTCCGACAGCGCCCGGCCCAGATCCCCACGCCTGCCAGG
  AGCAAGCCGAGAGCCAGCCGGCCGGCGCACTCCGACTCCGAGCAGTCTCTGTCCTTCGAC
  CCGAGCCCCGCGCCCTTTCCGGGACCCCTGCCCCGCGGGCAGCGCTGCCAACCTGCCGGC
  CATGGAGACCCCGTCCCAGCGGCGCGCCACCCGCAGCGGGGCGCAGGCCAGCTCCACTCC
  GCTGTCGCCCACCCGCATCACCCGGCTGCAGGAGAAGGAGGACCTGCAGGAGCTCAATGA
  TCGCTTGGCGGTCTACATCGACCGTGTGCGCTCGCTGGAAACGGAGAACGCAGGGCTGCG
  CCTTCGCATCACCGAGTCTGAAGAGGTGGTCAGCCGCGAGGTGTCCGGCATCAAGGCCGC
  CTACGAGGCCGAGCTCGGGGATGCCCGCAAGACCCTTGACTCAGTAGCCAAGGAGCGCGC
  CCGCCTGCAGCTGGAGCTGAGCAAAGTGCGTGAGGAGTTTAAGGAGCTGAAAGCGCGCAA
  TACCAAGAAGGAGGGTGACCTGATAGCTGCTCAGGCTCGGCTGAAGGACCTGGAGGCTCT
  GCTGAACTCCAAGAGGCCGCACTGAGCACTGCTCTCAGTGAGAAGCGCACGCTGGAGGGC
  GAGCTGCATGATCTGCGGGGCCAGGTGGCCAAGCTTGAGGCAGCCCTAGGTGAGGCCCAG
  AAAGCACTTCAGGATGAAATGCTGCGGCGGGTGGATGCTGANAACCAGCTGCACACATTG
  AGGAGGACTGGACTCCAGAAGACATCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_170707 unedited
  ACATTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCTTGTGTTTTTCCTTCGTATAAAACCA
  CTGTAGAAAATAACTTCTCTTAGAAAAAAAAATACAGAAGAAAAAAACCAAAGGGTTATT
  TTTCTTTGGCTTCAAGCCCCCTCCCATGAGGTGCAGGGCAGGGGGTGGGCATGAGGTGAG
  GAGGACGCAGGAAGCCTCCACCCCCACCCCTGCCTGGCAGGTCCCAGATTACATGATGCT
  GCAGTTCTGGGGGCTCTGGGTTCGGGGGCTGGAGTTGCCCAGGAGGTAGGAGCGGGTGAC
  CAGATTGTCCCCGAAGCTGCCACCCCCACTGCCCCCCACACTGCGGTAGCTGCGAGTGAC
  CGTGACACTGGAGGCAGAAGAGCCAGAGGAGATGGGTCCGCCCACCTGGGCTCCTGAGCC
  GCTGGCAGATGCCTTGTCGGCAGGCTGCCCGCAGGTCCCGCACAGCACGGTGCGCGAGCG
  CAGGTTGTACTCAGCGGGGTCCCCCGAGCTGCTGCAGTGGGAGCCGTGGTGGTGATGGAG
  CANGTCATCTCCATCCTCATCCTCGTCGTCCTCAACCACAGTCACTGAGCGCACCAGCTT
  GCGCATGGCCACTTCTTCCCCAGTGGGAGTGATGAGAGCCGTACGCAGGGCTGTTCCGCA
  GCCCCAGTTGTTCTGTGCCCTTCACACCAGGTCGGTAAGGGGGCTGTGGGTGGCCCCAAC
  TCCTTGCAGCCAGATCGTNACCACCTGCCCACCCTTCAGGGTGAACTTTGGTGGGGAACG
  GTAATTACCAAGGGATCATCTCCCATTCCGCCCCTTGATTTGGCCAATGCCAATGGAATG
  GGCCTAATTGGACTTGTTGCCCCACCGGACACAATTGGCTCTCCCGTCCTCTTCTTTCCG
  GCCCTCCCCCCCCCATCGGTCGTGTCTGTATACACCCCTTGCGCCCTCACAGTCCTCCCA
  TGTGCCCCTTTCCGGACCCTTCGCCACATTCTGTTTGCGCCCCCGCCACAGAAACACGTT
  TTCTCCCCCCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_170707
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170707.1, NP_733821.1
• RefSeq Size: 3181 bp
• RefSeq ORF: 1995 bp
• Locus ID: 4000
• UniProt ID: P02545
• Cytogenetics: 1q22
• Protein Families: Druggable Genome
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)
• Gene Summary: The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]
  Transcript Variant: This variant (1) encodes isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.