AIF (AIFM1) (NM_145813) Human Untagged Clone

CAT#: SC100268

AIFM1 (untagged)-Human apoptosis-inducing factor, mitochondrion-associated, 1 (AIFM1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AIF
• Synonyms: AIF; CMT2D; CMTX4; COWCK; COXPD6; NADMR; NAMSD; PDCD8
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_145813, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTCCGGTGTGGAGGCCTGGCGGCGGGTGCTTTGAAGCAGAAGCTGGTGCCCTTGGTGCGGACCGTGT
  GCGTCCGAAGCCCGAGGCAGAGGAACCGGCTCCCAGCTCGAGCCTTGGGCACAGAAGTGATTCAACTCTT
  CCCCGAGAAAGGAAATATGGGAAAGATCCTCCCCGAATACCTCAGCAACTGGACCATGGAAAAAGTCAGA
  CGAGAGGGGGTTAAGGTGATGCCCAATGCTATTGTGCAATCCGTTGGAGTCAGCAGTGGCAAGTTACTTA
  TCAAGCTGAAAGACGGCAGGAAGGTAGAAACTGACCACATAGTGGCAGCTGTGGGCCTGGAGCCCAATGT
  TGAGTTGGCCAAGACTGGTGGCCTGGAAATAGACTCAGATTTTGGTGGCTTCCGGGTAAATGCAGAGCTA
  CAAGCACGCTCTAACATCTGGGTGGCAGGAGATGCTGCATGCTTCTACGATATAAAGTTGGGAAGGAGGC
  GGGTAGAGCACCATGATCACGCTGTTGTGAGTGGAAGATTGGCTGGAGAAAATATGACTGGAGCTGCTAA
  GCCGTACTGGCATCAGTCAATGTTCTGGAGTGATTTGGGCCCCGATGTTGGCTATGAAGCTATTGGTCTT
  GTGGACAGTAGTTTGCCCACAGTTGGTGTTTTTGCAAAAGCAACTGCACAAGACAACCCCAAATCTGCCA
  CAGAGCAGTCAGGAACTGGTATCCGATCAGAGAGTGAGACAGAGTCCGAGGCCTCAGAAATTACTATTCC
  TCCCAGCACCCCGGCAGTTCCACAGGCTCCCGTCCAGGGGGAGGACTACGGCAAAGGTGTCATCTTCTAC
  CTCAGGGACAAAGTGGTCGTGGGGATTGTGCTATGGAACATCTTTAACCGAATGCCAATAGCAAGGAAGA
  TCATTAAGGACGGTGAGCAGCATGAAGATCTCAATGAAGTAGCCAAACTATTCAACATTCATGAAGACTG
  A
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145813 unedited
  NGGGGGTTCGAGATTTGTATACGATTCATATAGGGCGGCCGCGAATTCGCACGAGGTCGC
  CGTTCCCCTTCCCCGGCTCTAGCAGGCCGGCTTCTCTGTCCAATGCCCACCCGGAGCTGG
  GAGGAGGAGTCTGCGTAATGTGCGTGTGAAGAGACTGGGGGAGCTGGCCGGGGCTCACGG
  TGTTTGACCCGTCGGTCGTGCGTGAGAGGAAAGGGAAGGAGGAGGTCCCGAATAGCGGTC
  GCCGAAATGTTCCGGTGTGGAGGCCTGGCGGCGGGTGCTTTGAAGCAGAAGCTGGTGCCC
  TTGGTGCGGACCGTGTGCGTCCGAAGCCCGAGGCAGAGGAACCGGCTCCCAGGCAACTTG
  TTCCAGCGATGGCATGTTCCTCTAGAACTCCAGATGACAAGACAAATGGCTAGCTCTGGT
  GCATCAGGGGGCAAAATCGATAATTCTGTGTTAGTCCTTATTGTGGGCTTATCAACAGTA
  GGAGCTGGTGCCTATGCCTACAAGACTATGAAAGAGGATGAAAAAAGATACAATGAAAGA
  ATTTCAGGGTTAGGGCTGACACCAGAACAGAAACAGAAAAAGGCCGCGTTATCTGCTTCA
  GAAGGAGAGGAAGTTCCTCAAGACAAGGCGCCAAGTCATGTTCCTTTCCTGCTAATTGGT
  GGAGGCACAGCTGCTTTTGCTGCAGCCAGATCCATCCGGGCTCGGGATCCTGGGGCCAGG
  GTACTGATTGTATCTGAAGATCCTGAGCTGCCGTACATGCGACCTCCTCTTTCAAAAAAC
  TGTGGTTTTCAGATGACCCAAATGTCACAAAGACACTGCGATTCAAACAGTGGAATGGNA
  AAAGAGAAAGCATATATTTCCAGCCACCTTCTTCTATGTCTCTGCTCAGGACTGNCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145813 unedited
  GGCTCCTTTATGNACCGCGGTCCGCAATTCTANATCTCGAGTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAATCAAAATTTATTTCACTATGGGAA
  CCTTAAAAATTTACCTACCTAGTTGAAAATATTCACAAAGGACTTGATCATTCACACTCA
  TACCCAGAGAAAGTCTGCTGAATAAAAAAATGCTCCTTTACCCATTCGACCTCCTCTCAA
  GGGCTGCAGGGGGTTTGCCAATTCCACTGGGGGGCTTCAGTCTTCATGAATGTTGAATAG
  TTTGGCTACTTCATTGAGATCTTCATGCTACTCACCGGCCTTAATGATCTTCCTTGCTAT
  TGGCATTCGGTTAAAGATGTTCCATAACACAATCCCCACGACCACTTTGTCCCTGAGGTA
  AAAAATGACACCTTTGCCGTAGTCCTCCCCCTGGACGGGAGCCTGTGGAACTGCCGGGGT
  GCTGGGAGGAATAGTAATTTCTGAGGCCTCGGACTCTGTCTCACTCTCTGATCGGATACC
  AATTCCTGACTGCTCTGTGGCAAATTTGGGGTTGTCTTGTGCAGTTGCTTTTGCAAAAAC
  ACCAACTGTGGGCAAACTACTGTCCACAAGACCAATAGCTTCATAGCCAACATCGGGGCC
  CAAATCACTCCAGAACATTGACTGATGCCAGTACGGCTTAACAGCTCCAGTCATATTTTC
  TCCAGCCAATCNTCCACTCACACAGCGTGATCATGGTGCTCTACCCGCCTTCTTCCCAAT
  TTATATCGTAAAAGCCTGCAGCATCTCCTGCCACCCAAATGTTAAAGCGTGCTTGTAGCT
  CTGCCTTTACCCCGGAGCCACCCAAATCTGATCTATTTCCAGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145813
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145813.1, NP_665812.1
• RefSeq Size: 1354 bp
• RefSeq ORF: 981 bp
• Locus ID: 9131
• Cytogenetics: Xq26.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Apoptosis
• Gene Summary: This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and cognitive disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (3) lacks several in-frame exons in the 5' coding region compared to variant 1, resulting in an isoform (3) that is 287 aa shorter than isoform 1.