IGJ (JCHAIN) (NM_144646) Human Untagged Clone

CAT#: SC100076

IGJ (untagged)-Human immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides (IGJ)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IGJ
• Synonyms: IGCJ; IGJ; JCH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100076 sequence for NM_144646 edited (data generated by NextGen Sequencing)
  ATGAAGAACCATTTGCTTTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTG
  AAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAAATGTAAGTGTGCCCGGATT
  ACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAACATC
  CGAATTATTGTTCCTCTGAACAACAGGGAGAATATCTCTGATCCCACCTCACCATTGAGA
  ACCAGATTTGTGTACCATTTGTCTGACCTCTGTAAAAAATGTGATCCTACAGAAGTGGAG
  CTGGATAATCAGATAGTTACTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACA
  GAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCTGTGGTCCCACTCGTATAT
  GGTGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGATGCCTGCTATCCTGACTAA
  
  Clone variation with respect to NM_144646.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_144646 unedited
  GGGGTTCATATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGGTCAAGA
  TGAAGAACCATTTGCTTTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGA
  AAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAAATGTAAGTGTGCCCGGATTA
  CTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAACATCC
  GAATTATTGTTCCTCTGAACAACAGGGAGAATATCTCTGATCCCACCTCACCATTGAGAA
  CCAGATTTGTGTACCATTTGTCTGACCTCTGTAAAAAATGTGATCCTACAGAAGTGGAGC
  TGGATAATCAGATAGTTACTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAG
  AGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCTGTGGTCCCACTCGTATATG
  GTGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGATGCCTGCTATCCTGACTAAT
  TTAAGTCATTGCTGACTGCATAGCTCTTTTTCTTGAGAGGCTCTCCATTNTGATTCAGAA
  AGTTAGCATATTTATTACCAATGAAAATTGAACCAGNGCTTTTTTTTTTTTTTGGGTGAT
  GTAAAACCAACTTCCTGGCACCAAATATTAAAATAGTACATTGTTATCTTTATTAGGAAT
  CACTTCTTATTATATGTTCATACCTCTAGTATCAAAATTTTCCAATATTATGCTCACCTG
  AAAAAGTTTGCTCTCTAAGGATACAGTTCTAGCTTTAAACAATAACCAGGGGAGGAAATT
  AACCTCCAGGGTGAAAATTAGGAGGGGTAATAAAGGTTCCTGCATTTCCCCCCGGTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_144646
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144646.2, NP_653247.1
• RefSeq Size: 948 bp
• RefSeq ORF: 480 bp
• Locus ID: 3512
• UniProt ID: P01591
• Cytogenetics: 4q13.3
• Gene Summary: Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.[UniProtKB/Swiss-Prot Function]