SKA3 (NM_145061) Human Untagged Clone

SKU SC100066

SKA3 (untagged)-Human spindle and kinetochore associated complex subunit 3 (SKA3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: SKA3
• Synonyms: C13orf3; RAMA1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100066 sequence for NM_145061 edited (data generated by NextGen Sequencing); ATGGACCCTATCCGGAGCTTCTGCGGGAAGCTGCGGTCTCTGGCCAGCACGCTGGACTGC; GAGACGGCCCGGCTGCAGCGAGCGCTGGACGGAGAGGAAAGCGACTTTGAAGATTATCCA; ATGAGAATTTTATATGACCTTCATTCAGAAGTTCAGACTCTAAAGGATGATGTTAATATT; CTTCTTGATAAAGCAAGATTGGAAAATCAAGAAGGCATTGATTTCATAAAGGCAACAAAA; GTACTAATGGAAAAAAATTCAATGGATATTATGAAAATAAGAGAGTATTTCCAGAAGTAT; GGATATAGTCCACGTGTCAAGAAAAATTCAGTACACGAGCAAGAAGCCATTAACTCTGAC; CCAGAGTTGTCTAATTGTGAAAATTTTCAGAAGACTGATGTGAAAGATGATCTGTCTGAT; CCTCCTGTTGCAAGCAGTTGTATTTCTGAGAAGTCTCCACGTAGTCCACAACTTTCAGAT; TTTGGACTTGAGCGGTACATCGTATCCCAAGTTCTACCAAACCCTCCACAGGCAGTGAAC; AACTATAAGGAAGAGCCCGTAATTGTAACCCCACCTACCAAACAATCACTAGTAAAAGTA; CTAAAAACTCCAAAATGTGCACTAAAAATGGATGATTTTGAGTGTGTAACTCCTAAATTA; GAACACTTTGGTATCTCTGAATATACTATGTGTTTAAATGAAGATTACACAATGGGACTT; AAAAATGCGAGGAATAATAAAAGTGAGGAGGCCATAGATGCAGAATCCAGGCTCAATGAT; AATGTTTTTGCCACTCCCAGCCCCATCATCCAGCAGTTGGAAAAAAGTGATGCCGAATAT; ACCAACTCTCCTTTGGTACCTACATTCTGTACTCCTGGTTTGAAAATTCCATCTACAAAG; AACAGCATAGCTTTGGTATCCACAAATTACCCATTATCAAAAACAAATAGTTCATCAAAT; GATTTGGAAGTTGAAGATCGTACTTCGTTGGTTTTAAATTCAGACACATGCTTTGAGAAT; TTAACAGATCCCTCTTCACCTACGATTTCTTCTTATGAGAATCTGCTCAGAACACCTACA; CCTCCAGAAGTAACTAAAATTCCAGAAGATATTCTCCAGCTTTTATCAAAATACAACTCA; AACCTAGCTACTCCAATAGCAATTAAAGCAGTGCCACCCAGTAAAAGGTTCCTTAAACAT; GGACAGAACATCCGAGATGTCAGCAACAAAGAAAACTGA; ; Clone variation with respect to NM_145061.5; 760 a=>g;1086 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145061 unedited; NGGGTGCAGGATTTGTAACACGATTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTG; AGCGGTGGGGTCTGCGTACGCCTGGAGTCCTTCCCCGCTGTGCTCAGCATGGACCCTATC; CGGAGCTTCTGCGGGAAGCTGCGGTCTCTGGCCAGCACGCTGGACTGCGAGACGGCCCGG; CTGCAGCGAGCGCTGGACGGAGAGGAAAGCGACTTTGAAGATTATCCAATGAGAATTTTA; TATGACCTTCATTCAGAAGTTCAGACTCTAAAGGATGATGTTAATATTCTTCTTGATAAA; GCAAGATTGGAAAATCAAGAAGGCATTGATTTCATAAAGGCAACAAAAGTACTAATGGAA; AAAAATTCAATGGATATTATGAAAATAAGAGAGTATTTCCAGAAGTATGGATATAGTCCA; CGTGTCAAGAAAAATTCAGTACACGAGCAAGAAGCCATTAACTCTGACCCAGAGTTGTCT; AATTGTGAAAATTTTCAGAAGACTGATGTGAAAGATGATCTGTCTGATCCTCCTGTTGCA; AGCAGTTGTATTTCTGAGAAGTCTCCACGTAGTCCACAACTTTCAGATTTTGGACTTGAG; CGGTACATCGTATCCCAAGTTCTACCAAACCCTCCACAGGCAGTGAACAACTATAAGGAA; GAGCCCGTAATTGTAACCCCACCTACCAAACAATCACTAGTAAAAGTACTAAAAACTCCA; AAATGTGCACTAAAAATGGATGATTTTGAGTGTGTAACTCCTAAATTAGAACACTTTGGT; ATCTCTGATATACTATGTNGTTAAATGAAGATTACACNATGGGACTTAAAATGCGAGGAA; TATAAAAGTGAGAGGCATAGTGCAGAATCAGCTCATGATAATGTTTTGCCACTCCACCCA; TATNCAGAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145061 unedited; GTTAAAAACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTACAT; TATTTTTAAAAAAAATTATACTTACAACTACCCAGTAACTCCTAAAACAAGGACAAACTC; AAATTTTGTTTTGGGGACAGTTTTCTGACAAGATTAATAGAGCACAATGGACAGGTCCCC; CATTTGACTTTCAAGGAACAATCATAAAACAGGGGAATCTTTACTGGATCAAATTCCCAA; AACTACCCCTTTGAAGTAAGTTGACAAAAAACTTGGGGGTTCTTCTCAAATTTTTAAAGG; GTCCCCACGGGATGCTAGGGCCTTATTTTCTCATTGGCTGGAATTTGACAAGTTCTCCAA; AAGCCCTATCATGAACTTAATTAAATAATACTTTCCAGCTGGACTTAAATCAAAAGGATC; GTAGGCCTGATAAGCAGGTCAACCTGAAGCTACCAGGCAGCTCTGGAGGCTCCCGTGGCC; ATAGCTAACAGGGCACAGGAGGGCCCCTGGCCCAAGATGAGCCCTCAAAACCTTTTAAAG; AGAGTGAAATTCAGGGTCTACTGGGGGTCCAACCTGCAAAGTGGAGCCGGAGAGGTCAAC; TGCCATGTGATAAAAAGAGCTGACATAAGAGAAAAGACCCCCCCACAGTGAAAAGCTGGA; AGAAAAACAAAACTCACAACCTGACAGCAGAGTTCCTGGTTCCAACTGGTTGAGACCCGT; CTCTACCTCCAATGGCACAGTTTATAGTTCCCCAGGCCCTGGAAATCTCCCCCCTTTTCC; AAATTAATTTAACTGGNGGTTCCTGGTTGTTTGCAAACAAGAACTGGATCTTAAAAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_145061
• Insert Size: 2650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145061.3, NP_659498.2
• RefSeq Size: 2907 bp
• RefSeq ORF: 1239 bp
• Locus ID: 221150
• UniProt ID: Q8IX90
• Cytogenetics: 13q12.11
• Summary: This gene encodes a component of the spindle and kinetochore-associated protein complex that regulates microtubule attachment to the kinetochores during mitosis. The encoded protein localizes to the outer kinetechore and may be required for normal chromosome segregation and cell division. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.