Pla2g6 (NM_001270796) Rat Tagged ORF Clone

CAT#: RR217171

Pla2g6 (myc-DDK-tagged) - Rat phospholipase A2, group VI (cytosolic, calcium-independent) (Pla2g6), transcript variant 2

Product Images

Circular map for RR217171

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pla2g6
• Synonyms: iPla2; PNPLA9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217171 representing NM_001270796
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTTCTTTGGACGCCTCGTTAACACCCTCAGTAGTGTCACCAACTTGTTCTCAAACCCATTCCGGG
  TAAAGGAGGTGTCCTTGGCTGACTATGCCTCAAGTGAACGTGTTCGAGAGGAAGGGCAGCTGATCCTGTT
  GCAGAATGCTTCCAATCGTACCTGGGACTGTGTCCTGGTCAGCCCTAGGAACCCACAGAGTGGCTTCCGA
  CTCTTCCAACTGGAGTCAGAGGCAGACGCCCTGGTGAACTTCCAGCAGTATTCCTCCCAGCTGCCACCGT
  TCTATGAGAGCTCTGTGCAGGTCCTGCATGTGGAGGTGCTGCAGCACCTGACGGACCTCATTCGGAACCA
  CCCCAGCTGGACAGTGACGCACCTGGCCGTGGAACTTGGCATTCGGGAGTGTTTCCATCACAGCCGCATC
  ATCAGCTGCGCCAACAGCACAGAGAATGAGGAGGGCTGTACCCCACTACATCTGGCCTGCCGCAAGGGTG
  ACAGTGAGATTCTGGTGGAGTTGGTACAGTACTGCCACGCCCAGATGGATGTCACTGACAACAAAGGAGA
  GACCGCCTTCCATTATGCTGTGCAAGGGGACAATCCCCAGGTGCTACAGCTCCTAGGAAAGAATGCCTCA
  GCCGGCCTGAACCAGGTGAACAACCAAGGGCTGACGCCGCTGCACCTGGCCTGCCAGATGGGGAAGCAGG
  AGATGGTGCGTGTCCTGCTGCTCTGCAATGCCCGCTGCAACATCATGGGGCCCGGTGGCTTCCCCATCCA
  CACAGCCATGAAGTTTTCCCAGAAGGGGTGTGCTGAAATGATTATCAGCATGGACAGTAACCAGATCCAC
  AGCAAGGATCCTCGCTATGGAGCCAGCCCGCTCCACTGGGCCAAGAACGCTGAGATGGCCCGAATGCTTC
  TGAAGAGGGGCTGTGACGTGGACAGCACAAGTGCCTCGGGGAACACAGCCCTGCACGTGGCGGTGACGCG
  CAACCGCTTTGACTGTGTCATGGTGCTGCTGACCTATGGGGCCAACGCAGGTGCCCGAGGAGAGCATGGG
  AACACACCACTGCATCTGGCCATGTCGAAAGATAACATGGAGATGGTCAAAGCCCTCATTGTATTTGGGG
  CAGAAGTAGACACCCCGAATGACTTTGGGGAGACTCCTGCATTCATAGCTTCCAAGATCAGCAAGCAGCT
  TCAGGACCTCATGCCCGTCTCCCGAGCCCGGAAGCCAGCGTTCATCCTGAGCTCCATGAGGGATGAGAAG
  CGAAGCCATGACCACCTGCTCTGCTTGGATGGAGGGGGCGTGAAGGGCCTGGTCATCATCCAGCTTCTCA
  TTGCCATTGAGAAGGCCTCAGGTGTGGCTACCAAGGACCTCTTCGACTGGGTGGCAGGAACCAGCACAGG
  CGGCATCCTGGCCCTGGCCATTCTACACAGTAAATCCATGGCCTATATGCGTGGCGTGTACTTCCGTATG
  AAGGATGAGGTGTTTCGGGGCTCACGGCCCTATGAGTCTGGGCCCCTGGAGGAGTTCCTGAAGCGTGAGT
  TTGGGGAGCACACCAAGATGACAGATGTCAAGAAACCCAAGGTGATGCTGACAGGGACACTGTCAGACCG
  GCAGCCAGCAGAGCTCCACCTCTTCCGGAATTACGACGCCCCAGAGGCCGTTCGGGAACCTCGCTGTACC
  CCAAACATTAACCTGAAGCCGCCCACCCAGCCTGCAGACCAGTTAGTATGGCGGGCAGCCCGGAGCAGTG
  GGGCAGCCCCAACCTATTTCCGGCCCAATGGACGCTTCTTGGATGGTGGGCTGCTGGCCAACAACCCCAC
  ACTGGATGCCATGACTGAAATCCATGAGTACAATCAGGATATGATCCGCAAGGGCCAGGGCAACAAGGTG
  AAGAAACTCTCCATAGTCGTGTCTCTGGGGACAGGAAAGTCCCCTCAAGTGCCTGTAACCTGTGTAGATG
  TCTTTCGTCCCAGCAACCCTTGGGAACTGGCCAAGACTGTTTTTGGAGCCAAGGAACTGGGCAAGATGGT
  GGTGGACTGTTGCACAGATCCAGATGGGCGGGCTGTGGATCGTGCCCGGGCCTGGTGTGAGATGGTCGGT
  ATCCAGTACTTCAGGCTGAACCCCCAGCTAGGCTCAGACATCATGCTGGACGAGGTCAGTGATGCAGTGC
  TGGTCAACGCCCTCTGGGAGACCGAGGTCTACATCTATGAGCACCGGGAGGAGTTCCAGAAGCTTGTCCA
  GCTGCTGCTATCTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217171 representing NM_001270796
  Red=Cloning site Green=Tags(s)
  
  MQFFGRLVNTLSSVTNLFSNPFRVKEVSLADYASSERVREEGQLILLQNASNRTWDCVLVSPRNPQSGFR
  LFQLESEADALVNFQQYSSQLPPFYESSVQVLHVEVLQHLTDLIRNHPSWTVTHLAVELGIRECFHHSRI
  ISCANSTENEEGCTPLHLACRKGDSEILVELVQYCHAQMDVTDNKGETAFHYAVQGDNPQVLQLLGKNAS
  AGLNQVNNQGLTPLHLACQMGKQEMVRVLLLCNARCNIMGPGGFPIHTAMKFSQKGCAEMIISMDSNQIH
  SKDPRYGASPLHWAKNAEMARMLLKRGCDVDSTSASGNTALHVAVTRNRFDCVMVLLTYGANAGARGEHG
  NTPLHLAMSKDNMEMVKALIVFGAEVDTPNDFGETPAFIASKISKQLQDLMPVSRARKPAFILSSMRDEK
  RSHDHLLCLDGGGVKGLVIIQLLIAIEKASGVATKDLFDWVAGTSTGGILALAILHSKSMAYMRGVYFRM
  KDEVFRGSRPYESGPLEEFLKREFGEHTKMTDVKKPKVMLTGTLSDRQPAELHLFRNYDAPEAVREPRCT
  PNINLKPPTQPADQLVWRAARSSGAAPTYFRPNGRFLDGGLLANNPTLDAMTEIHEYNQDMIRKGQGNKV
  KKLSIVVSLGTGKSPQVPVTCVDVFRPSNPWELAKTVFGAKELGKMVVDCCTDPDGRAVDRARAWCEMVG
  IQYFRLNPQLGSDIMLDEVSDAVLVNALWETEVYIYEHREEFQKLVQLLLSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270796
• ORF Size: 2256 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270796.1, NP_001257725.1
• RefSeq Size: 2992 bp
• RefSeq ORF: 2259 bp
• Locus ID: 360426
• UniProt ID: P97570
• Cytogenetics: 7q34
• MW: 83.6 kDa
• Gene Summary: has Ca2+ independent phospholipase A2 activity; may play a role in regulating transmembrane ionic fluxes in pancreatic beta cells [RGD, Feb 2006]