Shkbp1 (NM_001271080) Rat Tagged ORF Clone

CAT#: RR217125

Shkbp1 (myc-DDK-tagged) - Rat Sh3kbp1 binding protein 1 (Shkbp1)

Product Images

Circular map for RR217125

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Shkbp1
• Synonyms: RGD1309281; Sb1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217125 representing NM_001271080
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTCCCGACGACTGCAGTGGAGGGGGTCCGGAGTCGGGGAGCGCCCGGGGAAGTGATTCATCTGA
  ACGTAGGGGGCAAGCGGTTCAGCACCTCTCGCCAGACGCTAACCTGGATCCCAGACTCCTTCTTTTCCAG
  TCTTTTGAGCGGACGTATCTCAACCCTGAAAGATGAGACCGGAGCCATCTTCATCGACCGGGACCCCACT
  GTCTTTGCCCCCATCCTCAATTTCCTGAGGACCAAAGAGTTAGACCCCAGGGGTGTCCATGGTTCCAGCC
  TCCTCCATGAAGCCCAATTCTATGGGCTCACTCCACTGGTTCGTCGCCTGCAGGTAAGGGAAGAGTTGGA
  TCGATCTTCCTGCGGAAATGTCCTCTTCAATGGTTATCTACCTCCTCCAGTGTTCCCAGTGAAGCGGAGA
  AACCGGCACAGCCTGGTGGGGCCCCAGCAAATAGGGGGACGCCCAGCCCCTGTTCGACGGAGCAACACGA
  TGCCCCCCAACTTGGGTAATGCAGGGCTGCTAGGCCGAATGCTGGATGATAGAGCACCTCCATCCCCATC
  AGGCCAACCAGAGGAACCAGGGATGGTGCGGCTGGTGTGTGGTCACCACAACTGGATTGCTGTGGCGTAT
  ACCCACTTTCTCGTCTGTTATAGACTGAAGGAAGCCTCCGGTTGGCAGCTTGCATTCTCCAGTCCTCGCC
  TAGACTGGCCCATTGAGCGCCTGGCTCTCACAGCTCGGGTGCTCGGTGGTGCTCCAGGCGAGCATGACAA
  GATGGTGGCTGCAGCCACAGGCAGCGAGATCCTTCTGTGGGCGCTACAGGCACAAGGTGGTGGCTCTGAG
  ATAGGTGTCTTCCACCTGGGTGTGCCTGTGGAAGCCTTGTTCTTTGTTGGGAACCAGCTCATTGCCACAA
  GCCACACAGGGCGCATCGGTGTCTGGAACGCCGTGACCAAGCACTGGCAGGTCCAGGAGGTCCAGCCTAT
  CACGAGTTATGATGCAGCCGGCTCCTTCCTCCTCCTGGGCTGCAGCAATGGCTCCATCTATTATGTAGAT
  GTGCAGAAATTCCCCCTGCGCATGAAGGACAACGACCTCCTTGTCAGTGAGCTCTACCGAGACCCAGCAG
  AAGATGGGGTCACCGCCCTCAGCGTCTACCTCACCCCCAAGACCAGTGACAGTGGGAACTGGATTGAGAT
  TGCCTATGGCACCAGCTCCGGAGTGGTGCGGGTCATTGTCCAGCACCCCGAGACAGTGGGCTCGGGACCC
  CAGCTCTTCCAGACCTTCAGTGTACACCGCAGCCCTGTCACCAAGATCATGCTGTCAGAGAAGCACCTCA
  TCTCTGTGTGTGCCGACAACAACCATGTTCGGACGTGGTCTGTGACCCGCTTTCGAGGCATGATCTCCAC
  CCAGCCTGGTTCCACTCCACTGGCATCCTTCAAGATCCTGGCACTTGAATCAGCCGATGGGCTCGGTGGC
  TGCAGTGCTGGCAATGACATTGGTCCCTATGGTGAGCGGGATGACCAGCAGGTGTTCATCCAGAAGGTGG
  TACCAAATGCCAGCCAGCTGTTTGTGCGTCTCTCCTCCACTGGGCAGCGGGTGTGTTCTGTACGCTCAGT
  GGATGGCTCCGCTACGACTGCCTTCACAGTGTTGGAGTGTGAGGGCTCCAGGCGGTTGGGCTCCAGACCC
  CGACGCTACCTGCTCACCGGCCAAGCCAATGGCAGCCTGGCCATGTGGGACCTGACCACTGCCATGGACG
  GCCTGGGCCAAACCCCTGCAGGCGGCCTGACCGAAGAAGAGCTGATGGACCAACTGGAGCAGTGTGAACT
  GAGCCCATTGACTTCTTCTCGGGCCTCTTTTCCCAGCCCCTCACCCAGAACCTCTCTTACCAGTCTACAC
  TCAGCCTCCAGCAACACCTCGCTGTGTGGCCACAGAGGGAGCCCCAGCCCACCGCAGGCAGGGGCCCGGA
  GCCGCGGGGCAGGAAGCTTCGTGGATCGGTTTAAAGAACTGGCAAGGGGTGCACCGGAGCTCCGAGGGCC
  ACCAACACCAGCCCCTCGACCCTCCACCAGTCTTGGGAATCCCCTGATACTTCCCAAAAACACACTTAAC
  GAAACTTCCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217125 representing NM_001271080
  Red=Cloning site Green=Tags(s)
  
  MAVPTTAVEGVRSRGAPGEVIHLNVGGKRFSTSRQTLTWIPDSFFSSLLSGRISTLKDETGAIFIDRDPT
  VFAPILNFLRTKELDPRGVHGSSLLHEAQFYGLTPLVRRLQVREELDRSSCGNVLFNGYLPPPVFPVKRR
  NRHSLVGPQQIGGRPAPVRRSNTMPPNLGNAGLLGRMLDDRAPPSPSGQPEEPGMVRLVCGHHNWIAVAY
  THFLVCYRLKEASGWQLAFSSPRLDWPIERLALTARVLGGAPGEHDKMVAAATGSEILLWALQAQGGGSE
  IGVFHLGVPVEALFFVGNQLIATSHTGRIGVWNAVTKHWQVQEVQPITSYDAAGSFLLLGCSNGSIYYVD
  VQKFPLRMKDNDLLVSELYRDPAEDGVTALSVYLTPKTSDSGNWIEIAYGTSSGVVRVIVQHPETVGSGP
  QLFQTFSVHRSPVTKIMLSEKHLISVCADNNHVRTWSVTRFRGMISTQPGSTPLASFKILALESADGLGG
  CSAGNDIGPYGERDDQQVFIQKVVPNASQLFVRLSSTGQRVCSVRSVDGSATTAFTVLECEGSRRLGSRP
  RRYLLTGQANGSLAMWDLTTAMDGLGQTPAGGLTEEELMDQLEQCELSPLTSSRASFPSPSPRTSLTSLH
  SASSNTSLCGHRGSPSPPQAGARSRGAGSFVDRFKELARGAPELRGPPTPAPRPSTSLGNPLILPKNTLN
  ETSF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271080
• ORF Size: 2112 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271080.1, NP_001258009.1
• RefSeq Size: 2300 bp
• RefSeq ORF: 2115 bp
• Locus ID: 292735
• UniProt ID: P0C5J9
• Cytogenetics: 1q21
• MW: 76 kDa
• Gene Summary: Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.[UniProtKB/Swiss-Prot Function]