Inpp5e (NM_053632) Rat Tagged ORF Clone

CAT#: RR217040

Inpp5e (myc-DDK-tagged) - Rat inositol polyphosphate-5-phosphatase E (Inpp5e)

Product Images

Circular map for RR217040

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Inpp5e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217040 representing NM_053632
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTCCAAGTCCGCTTGCCTGCGTCACACGGAGGCACCTGGGCAGCTAGAAGGCAGAATGCTCCAGG
  GACAGCTTTCGAACCCCGAAAAGAAGCTCATCCCAACATCAGCCTCCTTACCAGCTGCTGACTCTCAGAG
  TTCACAGACAAACTCCATGCCTCCTTTAAGCATGCCAGCAAAACCAAGCAACCAGAACCTACAAGCCAAG
  GCAAACCTCATCACCCCACAGCCACCCATTAGACCCAAGTTGGAGAGAACCCTGTCCCTCGATGACAAGG
  GCTGGAGAAGGAGGCGTTTCCGGGGTAGTCAGGAGGATCTGACCGTCCAGAATGGGGCCAGTCCCTGCAG
  GGGTTCCTTGCAGGACTCAGTAGCCCAGTCTCCTGCCTACAGTCGTCCCCTGCCCTGCCTCAGTACATCC
  TTACAGGAGATACCTAAGCCCCGAAGGGCCACAGGCAGCGAGGGAGGGAGCCCATCCTTGTGGAGTGACT
  GTCTTTCTGGAATGATCAGCACCTCCTTGGACCTCCTGCACAGAGAGGCTGCCTCAGGTGGGCCCCACTC
  CAGGCTGGCAAGTTTGCGTGCTACACACACACCGCCAGCTATGGACCTCAACATAGCCTCCAGCTCCCTG
  AGAACAGCAAACAAGGTTGACCCTGAGCACACGGACTACAAGCTCCGCATGCAGAACAGACTGGTCAGGG
  CCCACAGCAACCTGGGCCCTAGCAGACCCCGGAGTCCCCTGGCTGGTGACGACCACTCCATTCACTCAGC
  CAGATCCTCTTTCAGCCTTCTGGCCCCCATCCGCACCAAGGACATCAGGAGCAGGAGCTATCTGGAGGGA
  AGTCTTCTGGCCAGTGGGGCCCTGCTAGGAGCAGACGAGCTGGCCAGGTACTTCCCAGACCGAAACATGG
  CTCTTTTCGTGGCTACCTGGAACATGCAGGGCCAGAAGGAGCTTCCGGCAAGCCTGGATGAGTTTCTGCT
  TCCCACCGAGGCTGACTACACACAGGACCTGTATGTCATTGGCGTTCAGGAGGGCTGCTCTGACAGGCGG
  GAATGGGAGACACGCTTGCAGGAGACACTGGGCCCTCAGTATGTACTGCTGTCATCAGCAGCACATGGGG
  TCCTGTACATGTCCCTGTTTATCCGTAGGGACCTCATCTGGTTCTGCTCAGAGGTCGAGTACTCCACAGT
  AACTACACGCATCGTGTCTCAGATCAAGACCAAAGGGGCCCTGGGCGTCAGCTTCACCTTTTTTGGCACC
  TCCTTCCTCTTCATCACATCTCACTTCACCTCTGGAGATGGGAAGGTAGCAGAGCGGCTACTGGACTACA
  ACAGAACCATCCAAGCCCTCGCCTTGCCCCGGAATGTGCCAGACACAAACCCCTACCGCTCTAGTGCAGG
  GGATGTCACTACCCGGTTTGATGAGGTCTTCTGGTTTGGGGACTTCAACTTCCGCCTAAGTGGTGGACGA
  GTGGCTGTGGAGGCCTTCCTGAAGCAGGACCCAGAGGTGGATGTGCTGGCACTCCTGCAACATGACCAGC
  TCACCCGGGAGATGAAGAAAGGGTCCATCTTCAAGGGCTTTGAGGAGGCAGAAATTCACTTTCTCCCATC
  CTACAAGTTTGACATTGGGAAGGACACCTACGACAGCACCTCCAAGCAAAGGACACCCTCCTACACAGAC
  CGAGTCCTATACAAAAGCCGTCACAAGGGTGACATCTGTCCCATGAAGTATTCCTCTTGTCCTGGGATCA
  AGACTTCAGACCACCGTCCTGTGTATGGCTTGTTCCGGGTGAAAGTGAGGCCAGGACGAGACAACATCCC
  GTTAGCTGCCGGCAAGTTTGACCGAGAACTGTACTTGATAGGAATTAAAAGACGAATTTCGAAAGAGATT
  CAGAGACAAGAAGCACTGAAGAGTCAGAGCTCCAGTGCTGTGTGCACTGTGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217040 representing NM_053632
  Red=Cloning site Green=Tags(s)
  
  MPSKSACLRHTEAPGQLEGRMLQGQLSNPEKKLIPTSASLPAADSQSSQTNSMPPLSMPAKPSNQNLQAK
  ANLITPQPPIRPKLERTLSLDDKGWRRRRFRGSQEDLTVQNGASPCRGSLQDSVAQSPAYSRPLPCLSTS
  LQEIPKPRRATGSEGGSPSLWSDCLSGMISTSLDLLHREAASGGPHSRLASLRATHTPPAMDLNIASSSL
  RTANKVDPEHTDYKLRMQNRLVRAHSNLGPSRPRSPLAGDDHSIHSARSSFSLLAPIRTKDIRSRSYLEG
  SLLASGALLGADELARYFPDRNMALFVATWNMQGQKELPASLDEFLLPTEADYTQDLYVIGVQEGCSDRR
  EWETRLQETLGPQYVLLSSAAHGVLYMSLFIRRDLIWFCSEVEYSTVTTRIVSQIKTKGALGVSFTFFGT
  SFLFITSHFTSGDGKVAERLLDYNRTIQALALPRNVPDTNPYRSSAGDVTTRFDEVFWFGDFNFRLSGGR
  VAVEAFLKQDPEVDVLALLQHDQLTREMKKGSIFKGFEEAEIHFLPSYKFDIGKDTYDSTSKQRTPSYTD
  RVLYKSRHKGDICPMKYSSCPGIKTSDHRPVYGLFRVKVRPGRDNIPLAAGKFDRELYLIGIKRRISKEI
  QRQEALKSQSSSAVCTVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053632
• ORF Size: 1944 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053632.1, NP_446084.1
• RefSeq Size: 3342 bp
• RefSeq ORF: 1947 bp
• Locus ID: 114089
• UniProt ID: Q9WVR1
• Cytogenetics: 3p13
• MW: 72 kDa
• Gene Summary: hydrolyzes the 5-phosphate group of inositol 1,4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, and phosphatidylinositol 4, 5-bisphosphate [RGD, Feb 2006]