Ptpn11 (NM_001177593) Rat Tagged ORF Clone

CAT#: RR216979

Ptpn11 (myc-DDK-tagged) - Rat protein tyrosine phosphatase, non-receptor type 11 (Ptpn11), transcript variant 1

Product Images

Circular map for RR216979

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptpn11
• Synonyms: Shp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216979 representing NM_001177593
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACATCCCGGAGATGGTTTCACCCCAATATCACTGGTGTGGAGGCAGAGAATCTCCTGCTGACCCGAG
  GAGTCGATGGCAGTTTCTTAGCGAGGCCCAGCAAGAGTAACCCTGGAGACTTCACTCTGTCTGTTAGAAG
  AAATGGAGCCGTTACCCACATCAAGATTCAGAACACTGGGGACTACTATGACCTCTATGGCGGGGAAAAG
  TTTGCCACCTTGGCTGAACTGGTCCAGTATTACATGGAGCATCACGGGCAGCTGAAAGAGAAGAATGGAG
  ATGTTATTGAGCTCAAGTACCCACTGAACTGTGCAGACCCTACCTCTGAAAGGTGGTTCCACGGTCACTT
  GTCTGGAAAAGAAGCAGAGAAGCTGCTGACAGAGAAGGGGAAGCATGGCAGTTTCCTCGTCCGGGAGAGC
  CAGAGCCACCCTGGGGACTTCGTCCTCTCCGTCCGCACTGGTGATGACAAAGGGGAGAGCAATGACAGCA
  AGTCCAAAGTGACCCATGTCATGATCCGCTGTCAGGAGCTGAAATATGATGTTGGTGGAGGAGAGCGCTT
  TGACTCTTTGACAGACCTGGTGGAGCATTACAAGAAGAACCCCATGGTGGAGACACTGGGCACAGTCCTG
  CAGCTCAAACAGCCCCTCAACACAACTCGTATTAATGCCGCTGAAATCGAAAGCCGGGTTCGGGAGTTAA
  GCAAGCTAGCCGAGACCACAGATAAAGTCAAACAGGGCTTTTGGGAAGAATTTGAGACTCTACAGCAACA
  GGAATGCAAACTTCTCTACAGCCGAAAAGAAGGACAGAGACAAGAAAATAAAAACAAAAATAGATACAAA
  AACATCCTGCCCTTTGATCATACCAGGGTTGTCCTGCACGATGGGGATCCCAACGAGCCAGTTTCTGATT
  ACATCAATGCCAACATCATCATGCCTGAATTTGAAACCAAGTGCAACAATTCAAAACCCAAAAAGAGTTA
  CATTGCCACTCAAGGCTGCCTGCAGAACACGGTGAATGACTTCTGGCGGATGGTGTTCCAGGAGAACTCT
  CGAGTCATTGTCATGACCACAAAGGAAGTGGAGAGAGGGAAGAGCAAGTGTGTCAAGTACTGGCCTGATG
  AGTGTGCACTCAAAGAGTATGGCGTCATGCGTGTGAGGAACGTCAGAGAAAGTGCTGCGCATGACTACAC
  CTTACGAGAACTCAAACTCTCTAAGGTCGGACAAGCTCTACTCCAGGGAAACACAGAGAGAACCGTCTGG
  CAGTACCACTTTCGGACCTGGCCAGACCACGGTGTGCCTAGTGACCCTGGAGGTGTGCTGGACTTCCTGG
  AGGAGGTCCACCACAAGCAGGAGAGCATCGTGGATGCAGGCCCTGTCGTGGTTCACTGCAGTGCTGGGAT
  TGGCCGGACAGGAACGTTCATTGTGATTGATATCCTTATTGACATCATCCGAGAGAAAGGTGTGGACTGT
  GACATCGACGTTCCTAAAACCATTCAGATGGTACGGTCCCAGAGGTCAGGGATGGTCCAGACAGAAGCAC
  AGTACCGGTTCATCTACATGGCCGTCCAGCACTACATAGAGACGCTGCAACGCAGGATCGAGGAGGAGCA
  GAAAAGCAAAAGGAAAGGACATGAATATACCAATATTAAGTATTCCCTGGTGGACCAGACAAGTGGCGAT
  CAGAGTCCCCTGCCACCTTGCACCCCAACGCCACCCTGTGCGGAAATGCGGGAGGACAGCGCTCGAGTCT
  ATGAGAACGTGGGCCTCATGCAGCAGCAGAGGAGTTTCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216979 representing NM_001177593
  Red=Cloning site Green=Tags(s)
  
  MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEK
  FATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRES
  QSHPGDFVLSVRTGDDKGESNDSKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVL
  QLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYK
  NILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENS
  RVIVMTTKEVERGKSKCVKYWPDECALKEYGVMRVRNVRESAAHDYTLRELKLSKVGQALLQGNTERTVW
  QYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIVDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDC
  DIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLVDQTSGD
  QSPLPPCTPTPPCAEMREDSARVYENVGLMQQQRSFR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177593
• ORF Size: 1791 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177593.1, NP_001171064.1
• RefSeq Size: 5538 bp
• RefSeq ORF: 1794 bp
• Locus ID: 25622
• UniProt ID: P41499
• Cytogenetics: 12q16
• MW: 68.5 kDa
• Gene Summary: catalyzes the tyrosine dephosphorylation of para-nitrophenylphosphate, nicotinic acetylcholine receptor, myelin basic protein, and other proteins [RGD, Feb 2006]