Apoe (NM_001270682) Rat Tagged ORF Clone

CAT#: RR216320

  • TrueORF®

Apoe (myc-DDK-tagged) - Rat apolipoprotein E (Apoe), transcript variant 3


  "NM_001270682" in other vectors (1)

Reconstitution Protocol

USD 330.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Other products for "Apoe"

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Apoe
Synonyms APOEA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR216320 representing NM_001270682
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
GCC
C

ATGAAGGCTCTGTGGGCCCTGCTGTTGGTCCCATTGCTGACAGGATGCCTGGCCGAGGGAGAGCTGGAGG
TGACAGATCAGCTCCCAGGGCAAAGCGACCAACCCTGGGAGCAGGCCCTGAACCGCTTCTGGGATTACCT
GCGCTGGGTGCAGACGCTTTCTGACCAGGTCCAGGAAGAGCTGCAGAGCTCCCAAGTCACACAGGAACTG
ACGGTACTGATGGAGGACACTATGACGGAAGTAAAGGCATACAAAAAGGAGCTGGAGGAACAGCTGGGCC
CAGTGGCGGAGGAGACACGGGCCAGGCTGGCTAAAGAGGTGCAGGCGGCACAGGCCCGTCTGGGAGCTGA
CATGGAGGATCTACGCAACCGACTCGGGCAGTACCGCAACGAGGTAAACACCATGCTGGGCCAGAGCACA
GAGGAGCTGCGGTCGCGCCTCTCCACACACCTGCGCAAGATGCGCAAGCGCCTGATGCGGGATGCGGATG
ATCTGCAGAAGCGCCTGGCGGTGTACAAGGCCGGGGCACAGGAGGGCGCCGAGCGCGGTGTGAGTGCTAT
CCGTGAGCGCCTGGGGCCACTGGTGGAGCAGGGTCGTCAGCGCACAGCCAACCTAGGCGCTGGCGCCGCC
CAGCCCCTGCGCGATCGCGCCCAGGCTTTGAGTGACCGCATCCGAGGGCGGCTGGAGGAAGTGGGCAACC
AGGCCCGAGACCGCCTAGAGGAGGTGCGTGAGCAGATGGAGGAGGTGCGCTCCAAGATGGAGGAGCAGAC
CCAGCAGATACGCCTGCAGGCCGAGATCTTCCAGGCCCGCATCAAGGGCTGGTTCGAGCCGCTAGTGGAA
GACATGCAGCGCCAGTGGGCAAACCTAATGGAGAAGATACAGGCCTCTGTGGCTACCAACTCCATTGCCT
CCACCACAGTGCCCCTGGAGAATCAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR216320 representing NM_001270682
Red=Cloning site Green=Tags(s)

MKALWALLLVPLLTGCLAEGELEVTDQLPGQSDQPWEQALNRFWDYLRWVQTLSDQVQEELQSSQVTQEL
TVLMEDTMTEVKAYKKELEEQLGPVAEETRARLAKEVQAAQARLGADMEDLRNRLGQYRNEVNTMLGQST
EELRSRLSTHLRKMRKRLMRDADDLQKRLAVYKAGAQEGAERGVSAIRERLGPLVEQGRQRTANLGAGAA
QPLRDRAQALSDRIRGRLEEVGNQARDRLEEVREQMEEVRSKMEEQTQQIRLQAEIFQARIKGWFEPLVE
DMQRQWANLMEKIQASVATNSIASTTVPLENQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites AscI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001270682
ORF Size 936 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001270682.1, NP_001257611.1
RefSeq Size 1191 bp
RefSeq ORF 939 bp
Locus ID 25728
UniProt ID P02650
Cytogenetics 1q21
MW 35.8 kDa
Gene Summary plays a role in plasma lipoprotein transport [RGD, Feb 2006]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.