Snip1 (NM_001014069) Rat Tagged ORF Clone

CAT#: RR215675

Snip1 (Myc-DDK-tagged ORF) - Rat Smad nuclear interacting protein 1 (Snip1), (10 ug)

Product Images

Circular map for RR215675

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Snip1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215675 representing NM_001014069
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGCGGGGAAGAGCGAGCGGGAGCGAAGCAGCCGGCGGCGACACCGGTCAGGCGACGCTTTGGCGA
  CCGTAGTAGTGAAGCAGGAACGTCTAAGCCCCGAACCCGTCGCACACCGCCGACCTGACGCTCCAGCCGC
  TAGCCCGCCCCCGCCCGCCGCCGAGTCGGGCTCGGCTGGTCACCGCGGGAGCCGAGCCCGGGGAGCGAGC
  CGGTCCCCAGCCAAAAAGAAAAGCAAGTCCTCCGGCAGAAGAAGCAAGTCTCCTCGGACTAAGAGAAGCC
  GGAGTCCCCACTACTCCACGGTCAAAGTGAAGCAGGAACGGGAGGACCATCCACGGAGAGGACGAGAGGA
  TAGGCAGCACCGGGAGTTATCAGAACAGGAGCACAGGAGAGCACGGAACAGCGAGCGAGATCGGCACCGG
  GGCCATGCCCGCCAAAGGAGGAGCTCTGATGAGAGGCCTGTCAGTGGGCAGGGTCGGGATAGAGACAGCC
  AGATCCTGCAGGCCCAGGAAGAAGAGAGGGACTTTAATAATGCCAGGCGCCGGGAGCACCGCCAGCAGAA
  TGAGAGTGCTGGCGCTGAGGCTCAGGAGGTGATCCCTCGCCCTGCTGGTAACAAGAACAAAGAGGTGCCT
  GTTAAAGAAAAACCAAGCTTTGAACTTTCTGGGGCACTTCTTGAGGACACTAATACCTTCCGGGGTGTGG
  TCATTAAATACAGTGAGCCCCCAGAAGCCCGCATCCCCAAAAAACGGTGGCGTCTCTACCCCTTTAAAAA
  TGATGAGGTGCTTCCAGTCATGTACATCCATCGGCAGAGTGCGTACCTGCTGGGGAGACATCGCCGCATC
  GCGGACATTCCTATCGACCATCCCTCTTGCTCAAAGCAACATGCAGTCTTCCAGTACCGGCTTGTGGAGT
  ACACCCGTGCTGATGGGACGGTTGGTCGGAGGGTGAAGCCCTACATCATTGACCTTGGCTCAGGCAACGG
  AACATTCTTGAACAACAAGCGTATTGAGCCACAGAGATACTATGAACTGAAAGAAAAAGATGTACTTAAA
  TTTGGGTTCAGCAGCAGAGAATATGTCTTGCTTCATGAGTCTTCAGACACCTCTGAACTTGACAGGAAGG
  AAGATGAAGATGAGGAGGAGGAGGAGGAAATGGTGTCTGACAGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215675 representing NM_001014069
  Red=Cloning site Green=Tags(s)
  
  MKAGKSERERSSRRRHRSGDALATVVVKQERLSPEPVAHRRPDAPAASPPPPAAESGSAGHRGSRARGAS
  RSPAKKKSKSSGRRSKSPRTKRSRSPHYSTVKVKQEREDHPRRGREDRQHRELSEQEHRRARNSERDRHR
  GHARQRRSSDERPVSGQGRDRDSQILQAQEEERDFNNARRREHRQQNESAGAEAQEVIPRPAGNKNKEVP
  VKEKPSFELSGALLEDTNTFRGVVIKYSEPPEARIPKKRWRLYPFKNDEVLPVMYIHRQSAYLLGRHRRI
  ADIPIDHPSCSKQHAVFQYRLVEYTRADGTVGRRVKPYIIDLGSGNGTFLNNKRIEPQRYYELKEKDVLK
  FGFSSREYVLLHESSDTSELDRKEDEDEEEEEEMVSDSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014069
• ORF Size: 1167 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014069.1, NP_001014091.1
• RefSeq Size: 2338 bp
• RefSeq ORF: 1170 bp
• Locus ID: 313588
• UniProt ID: Q5M9G6
• Cytogenetics: 15p14
• MW: 44.9 kDa
• Gene Summary: Required for pre-mRNA splicing as component of the spliceosome. Down-regulates NF-kappa-B signaling by competing with RELA for CREBBP/EP300 binding. Involved in the microRNA (miRNA) biogenesis. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.[UniProtKB/Swiss-Prot Function]