Srek1 (NM_020092) Rat Tagged ORF Clone

CAT#: RR215519

Srek1 (Myc-DDK-tagged ORF) - Rat splicing factor, arginine/serine-rich 12 (Sfrs12), (10 ug)

Product Images

Circular map for RR215519

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Srek1
• Synonyms: Sfrs12; Srrp86; Srsf12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215519 representing NM_020092
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAGCCTGGTGCCTGGTGCAGGGCTGCTTCCCATACCAACCTCCAGTCCACTGACGGCAGTGAGTA
  GTCTTGGCGTTTCACTTAGCAGTTTGGGAGCCATACCGGCAGCAGCACTGGACCCAAACATCACAGCACT
  TGGAGAGATACCACAGCCACCACTTATGGGAAATGTGGATCCTTCTAAAATTGACGAGATTAGGAGAACA
  GTTTATGTTGGAAATTTGAATTCACAGACAACGACAGCTGATCAACTACTTGAATTTTTTAAACAAGTTG
  GAGAAGTGAAGTTTGTTCGGATGGCAGGTGATGAGACTCAGCCAACTCGGTTTGCTTTTGTGGAATTTGC
  AGACCAAAATTCTGTACCAAGGGCCCTTGCTTTTAATGGAGTTATGTTTGGAGACAGGCCGCTGAAAATA
  AATCACTCCAACAATGCAATAGTAAAACCTCCTGAGATGACACCTCAGGCTGCAGCTAAGGAGTTAGAAG
  AAGTAATGAAGCGAGTGCGGGAGGCTCAGTCATTTATCTCAGCAGCCATTGAACCAGAGTCTGGAAAGAG
  CAATGAAAGGAAAGGCGGTCGATCTCGTTCCCACACTCGCTCAAAGTCCAGGTCTAGCTCAAAGTCCCAT
  TCTAGAAGGAAGAGATCGCAGTCAAAGCACAGGAGTAGATCCCATAACAGGTCACGTTCAAGACAGAAAG
  ATAGAGCTAGATCCAAGAGCCCACATAAGAAACGCTCTAAGTCTAGGGAGAGGCGGAAGTCAAGGAGCCG
  GTCGCGGTCACGGGACAAGAGAAAAGATACCCGGGAAAAGGTGAAGGAAAGAGTGAAGGAGAGAGAAAGG
  GAAAAGGAAAAGGAAAGGGAAAAGGAGCGTGAAAAAGACAAGGAACGGGGTAAAAACAAAGATAAAGACC
  GTGAGAAGGAAAAGGACCATGAAAAGGAGCGAGACAAAGAAAAGGAGAAGGAACAAGATAAAGACAAGGA
  ACGGGAAAAAGACAGATCCAAAGAGGCAGATGAAAAACGGAAGAAGGAGAAGAAGTCTAGAACACCGCCC
  CGAAGCTACAATTCCTCAAGACGATCCCGTAGTGCCAGCAGGGAAAGGCGGAGGAGGAGAAGCAGGAGTT
  CTTCTAGATCGCCAAGAACATCAAAGACCATAAAAAGAAAGTCTTCTAGGTCTCCGTCTCCCAGGGGCCG
  GAATAAGAAAGAGAAAAAGAGAGAGAAAGAACGGGACCACATCAGTGACAGGAGAGAAAGAGAGCGTTCC
  ACCTCCACAAAAAAGAGTTCTAGTGACCGAGACGGGAAGGAGAAGGTGGAGAAGACTAACACACCAGTTA
  AGGAGAAGGAACACAGTAAAGAGGCAGATTCAACTGTGAGCACCGATGCGGATGAGAAGGACACTGCCAG
  GACTGAGGAGGAAAGTAAAGCACAGCACAATGGGAATTGCCAGCCCCACGAAGAGCGCCTGTGCAGCACG
  GCCGACGCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215519 representing NM_020092
  Red=Cloning site Green=Tags(s)
  
  MTSLVPGAGLLPIPTSSPLTAVSSLGVSLSSLGAIPAAALDPNITALGEIPQPPLMGNVDPSKIDEIRRT
  VYVGNLNSQTTTADQLLEFFKQVGEVKFVRMAGDETQPTRFAFVEFADQNSVPRALAFNGVMFGDRPLKI
  NHSNNAIVKPPEMTPQAAAKELEEVMKRVREAQSFISAAIEPESGKSNERKGGRSRSHTRSKSRSSSKSH
  SRRKRSQSKHRSRSHNRSRSRQKDRARSKSPHKKRSKSRERRKSRSRSRSRDKRKDTREKVKERVKERER
  EKEKEREKEREKDKERGKNKDKDREKEKDHEKERDKEKEKEQDKDKEREKDRSKEADEKRKKEKKSRTPP
  RSYNSSRRSRSASRERRRRRSRSSSRSPRTSKTIKRKSSRSPSPRGRNKKEKKREKERDHISDRRERERS
  TSTKKSSSDRDGKEKVEKTNTPVKEKEHSKEADSTVSTDADEKDTARTEEESKAQHNGNCQPHEERLCST
  ADAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020092
• ORF Size: 1482 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020092.1, NP_064477.1
• RefSeq Size: 1485 bp
• RefSeq ORF: 1485 bp
• Locus ID: 56763
• UniProt ID: Q9JKL7
• Cytogenetics: 2q12
• MW: 56.8 kDa
• Gene Summary: comprises of a single amino-terminal RNA recognition motif and two carboxy-terminal domains rich in serine-arginine (SR) dipeptides [RGD, Feb 2006]