Snx17 (NM_001011981) Rat Tagged ORF Clone

CAT#: RR215439

Snx17 (Myc-DDK-tagged ORF) - Rat sorting nexin 17 (Snx17), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Snx17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215439 representing NM_001011981
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTTTTCCATTCCTGAAACCGAGTCCCGCAGCGGGGACAGCGGCGGCTCCACCTACGTGGCCTATA
  ACATTCACGTGAATGGAGTCCTGCACTGTCGTGTGCGCTACAGCCAGCTCCTGGGGCTGCACGAGCAGCT
  TCGGAAGGAGTATGGGGCCAATGTGCTTCCTGCATTCCCCCCAAAGAAGCTTTTCTCTTTGACGCCTGCA
  GAGGTAGAACAGAGGAGGGAACAGTTAGAGAAGTACATGCAAGCTGTTCGGCAAGATCCATTGCTTGGGA
  GCAGTGAGACCTTTAATAGCTTTCTACGTCGGGCCCAACAGGAGACACAACAGGTCCCCACAGAGGAGGT
  TTCCTTGGAAGTGTTGCTCAGTGACGGACAGAAAGTTCTGGTCAATGTGCTAACTTCTGACCAGACGGAA
  GACGTCCTGGAGGCCGTGGCTGCAAAGCTGGATCTTCCAGATGACTTGATCGGATATTTTAGTCTCTTCC
  TTGTTCGAGAAAAAGAAGATGGCGCCTTTTCTTTTGTACGGAAGCTTCAGGAGTTTGAGCTGCCTTACGT
  ATCTGTCACCAGTCTTCGGAGTCAAGAGTATAAGATCGTGCTCAGAAAGAGTTACTGGGACTCTGCCTAT
  GACGACGATGTCATGGAGAACCGGGTTGGCCTGAACCTGCTTTATGCCCAGACGGTGTCAGACATCGAGC
  ATGGCTGGATCCTGGTCACCAAGGAGCAGCACCGACAGCTCAAATCTCTGCAAGAGAAGGTCTCCAAAAA
  GGAGTTCTTGCGGCTGGCCCAGACTCTGCGGCACTATGGCTATCTGCGCTTTGATGCCTGTGTGGCTGAC
  TTTCCGGAGAAGGACTGCCCTGTTGTGGTCAGTGCAGGCAACAGTGAGCTCAGTCTCCAGCTGCGACTCC
  CAGGCCAGCAGCTCCGTGAAGGCTCTTTCCGGGTCACCCGAATGCGGTGCTGGCGGGTCACCTCCTCTGT
  GCCACTCCCCAGTGGGGGTACAAGCACTCCAAGCCGGGGTCGGGGTGAGGTACGCCTGGAACTGGCTTTT
  GAATACCTCATGAGCAAGGACCGGCTACAGTGGGTCACCATCACCAGCCCACAGGCTATCATGATGAGCA
  TCTGCCTGCAGTCCATGGTAGATGAGCTGATGGTGAAGAAGTCTGGGGGCAGTATCAGGAAGATGCTGCG
  TCGGAGGGTGGGGGGCAACCTGAGACGTTCAGACAGCCAGCAAGCAGTGAAGTCCCCACCATTGCTCGAA
  TCACCAGATGCCAGCCGGGAATCCATGGTCAAACTCTCAAGTAAACTGAGTGCGGTGAGCTTGCGGGGAA
  TTGGCAGTCCCAGCACAGATGCCAGTGCCAGTGCCGTCCACGGCAATTTCGCCTTCGAGGGCATTGGAGA
  TGAGGATCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215439 representing NM_001011981
  Red=Cloning site Green=Tags(s)
  
  MHFSIPETESRSGDSGGSTYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVLPAFPPKKLFSLTPA
  EVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRAQQETQQVPTEEVSLEVLLSDGQKVLVNVLTSDQTE
  DVLEAVAAKLDLPDDLIGYFSLFLVREKEDGAFSFVRKLQEFELPYVSVTSLRSQEYKIVLRKSYWDSAY
  DDDVMENRVGLNLLYAQTVSDIEHGWILVTKEQHRQLKSLQEKVSKKEFLRLAQTLRHYGYLRFDACVAD
  FPEKDCPVVVSAGNSELSLQLRLPGQQLREGSFRVTRMRCWRVTSSVPLPSGGTSTPSRGRGEVRLELAF
  EYLMSKDRLQWVTITSPQAIMMSICLQSMVDELMVKKSGGSIRKMLRRRVGGNLRRSDSQQAVKSPPLLE
  SPDASRESMVKLSSKLSAVSLRGIGSPSTDASASAVHGNFAFEGIGDEDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011981
• ORF Size: 1410 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011981.1, NP_001011981.1
• RefSeq Size: 1944 bp
• RefSeq ORF: 1413 bp
• Locus ID: 298836
• UniProt ID: Q6AYS6
• Cytogenetics: 6q14
• MW: 52.9 kDa
• Gene Summary: Critical regulator of endosomal recycling of numerous surface proteins, including integrins, signaling receptor and channels. Binds to NPxY sequences in the cytoplasmic tails of target cargos. Associates with retriever and CCC complexes to prevent lysosomal degradation and promote cell surface recycling of numerous cargos such as integrins ITGB1, ITGB5 and their associated alpha subunits. Also required for maintenance of normal cell surface levels of APP and LRP1. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)).[UniProtKB/Swiss-Prot Function]