Bnip3l (NM_080888) Rat Tagged ORF Clone

CAT#: RR215204

Bnip3l (Myc-DDK-tagged ORF) - Rat BCL2/adenovirus E1B interacting protein 3-like (Bnip3l), (10 ug)

Specifications

Product Data

• Product Name: Bnip3l (NM_080888) Rat Tagged ORF Clone
• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bnip3l
• Synonyms: Nix; UV93
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215204 representing NM_080888
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCACTTAGTCGAGCCGCCGCCGCCCCTGCACAACAACAACAACAACTGCGAGGAAGGGGAGCAGT
  CCCTGCCGCCGCCCGCTGGCCTCAACAGTTCCTGGGTGGAGCTACCCATGAACAGCAGCAATGGCAACGG
  TAATGGAAATGGGAAGAATGGGGGCCTGGAGCACGTTCCTTCCTCGTCTTCCATCCACAATGGAGACATG
  GAGAAGATCCTTCTGGATGCGCAGCATGAGTCGGGACAGAGCAGCTCAAGAGGCAGTTCTCACTGTGACA
  GCCCTTCACCACAAGAAGACGGGCAAATAATGTTTGATGTTGAGATGCACACCAGCAGGGACCACAGCTC
  TCAGTCAGAAGAAGAAGTTGTAGATGGAGAAAAAGAAGTTGAGGCTTTGAAGAAAAGTGCAGACTGGGTA
  TCAGACTGGTCCAGTAGACCCGAAAACATCCCACCCAAAGAGTTCCATTTCAGACACCCTAAGCGTGCAG
  CCTCTCTAAGCATGAGGAAGAGTGGAGCCATGAAGAAAGGGGGCATTTTCTCTGCAGAGTTCCTGAAGGT
  CTTCATCCCATCTCTCTTCCTCTCTCACGTGTTGGCTTTGGGGCTGGGCATCTATATCGGAAAACGACTG
  AGCACACCTTCTGCCAGCACCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215204 representing NM_080888
  Red=Cloning site Green=Tags(s)
  
  MSHLVEPPPPLHNNNNNCEEGEQSLPPPAGLNSSWVELPMNSSNGNGNGNGKNGGLEHVPSSSSIHNGDM
  EKILLDAQHESGQSSSRGSSHCDSPSPQEDGQIMFDVEMHTSRDHSSQSEEEVVDGEKEVEALKKSADWV
  SDWSSRPENIPPKEFHFRHPKRAASLSMRKSGAMKKGGIFSAEFLKVFIPSLFLSHVLALGLGIYIGKRL
  STPSASTY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• Tag: Myc-DDK
• ACCN: NM_080888
• ORF Size: 654 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080888.1, NP_543164.1
• RefSeq Size: 3219 bp
• RefSeq ORF: 657 bp
• Locus ID: 140923
• Cytogenetics: 15p12
• MW: 23.7 kDa
• Gene Summary: may be involved in oligodendroglial lineage cell differentiation; may be involved in apoptosis [RGD, Feb 2006]