Nr2f1 (NM_031130) Rat Tagged ORF Clone

CAT#: RR215036

Nr2f1 (Myc-DDK-tagged ORF) - Rat nuclear receptor subfamily 2, group F, member 1 (Nr2f1), (10 ug)

Product Images

Circular map for RR215036

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nr2f1
• Synonyms: COUP-TFI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215036 representing NM_031130
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAATGGTAGTTAGCAGCTGGCGAGATCCGCAGGACGACGTGGCCGGGGGCAACCCCGGCGGCCCCA
  ACCCCGCAGCGCAGGCAGCCCGCGGCGGCGGCGGCGGCGAGCAACAGCAGGCGGGCTCCGGCGCGCCGCA
  CACGCCGCAGACCCCGGGCCAGCCCGGAGCGCCCGCCACCCCCGGCACGGCAGGGGACAAGGGCCAGGGC
  CCGCCCGGTTCAGGCCAGAGCCAGCAGCACATCGAGTGCGTGGTGTGCGGGGACAAGTCGAGCGGCAAGC
  ACTACGGCCAATTCACCTGCGAGGGCTGCAAAAGTTTCTTCAAGAGGAGCGTCCGCAGGAACTTAACTTA
  CACATGCCGTGCCAACAGGAACTGTCCCATCGACCAGCACCACCGCAACCAGTGCCAATACTGCCGCCTC
  AAGAAGTGCCTCAAAGTGGGCATGAGGCGGGAAGCGGTTCAGCGAGGAAGAATGCCTCCAACCCAGCCCA
  ATCCAGGCCAGTACGCACTCACAAACGGGGATCCCCTCAATGGCCACTGCTACCTGTCCGGCTACATCTC
  CCTGCTGCTGCGAGCAGAGCCCTACCCCACGTCGCGTTATGGCAGCCAGTGCATGCAGCCCAACAACATC
  ATGGGCATCGAGAACATCTGCGAGCTGGCAGCCCGCCTCCTCTTCAGCGCCGTCGAGTGGGCCCGCAACA
  TCCCGTTCTTCCCGGATCTGCAGATCACCGACCAGGTGTCCCTCCTACGCCTCACCTGGAGCGAGCTGTT
  CGTGCTCAACGCGGCCCAGTGCTCCATGCCCCTGCACGTGGCCGTGCTGGCAGCAGCCGGCCTACACGCC
  TCGCCCATGTCCGCGGACCGGGTCGTGGCCTTCATGGACCACATCCGCATCTTTCAGGAACAGGTGGAGA
  AGCTCAAGGCGCTGCACGTCGACTCTGCCGAGTACAGCTGCCTCAAAGCCATCGTGCTGTTCACGTCAGA
  TGCTTGTGGCCTGTCGGATGCTGCACACATCGAAAGCCTGCAGGAGAAATCGCAGTGTGCACTGGAGGAA
  TACGTGAGGAGCCAGTACCCCAACCAGCCCAGCCGCTTTGGCAAACTGCTGCTGCGATTGCCCTCTCTTC
  GCACAGTGTCCTCTTCTGTCATAGAGCAGCTCTTCTTCGTCCGTTTGGTAGGTAAAACCCCCATCGAAAC
  TCTCATCCGAGACATGTTGTTGTCAGGGAGCAGTTTCAACTGGCCTTACATGTCCATCCAGTGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215036 representing NM_031130
  Red=Cloning site Green=Tags(s)
  
  MAMVVSSWRDPQDDVAGGNPGGPNPAAQAARGGGGGEQQQAGSGAPHTPQTPGQPGAPATPGTAGDKGQG
  PPGSGQSQQHIECVVCGDKSSGKHYGQFTCEGCKSFFKRSVRRNLTYTCRANRNCPIDQHHRNQCQYCRL
  KKCLKVGMRREAVQRGRMPPTQPNPGQYALTNGDPLNGHCYLSGYISLLLRAEPYPTSRYGSQCMQPNNI
  MGIENICELAARLLFSAVEWARNIPFFPDLQITDQVSLLRLTWSELFVLNAAQCSMPLHVAVLAAAGLHA
  SPMSADRVVAFMDHIRIFQEQVEKLKALHVDSAEYSCLKAIVLFTSDACGLSDAAHIESLQEKSQCALEE
  YVRSQYPNQPSRFGKLLLRLPSLRTVSSSVIEQLFFVRLVGKTPIETLIRDMLLSGSSFNWPYMSIQCS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031130
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031130.1, NP_112392.1
• RefSeq Size: 2514 bp
• RefSeq ORF: 1260 bp
• Locus ID: 81808
• Cytogenetics: 2q11
• MW: 45.8 kDa
• Gene Summary: acts as a transcriptional activator; inhibits neurite outgrowth; may regulate cell surface interactions during neurogenesis [RGD, Feb 2006]