Pycr2 (NM_001012208) Rat Tagged ORF Clone
CAT#: RR214930
- TrueORF®
Pycr2 (Myc-DDK-tagged ORF) - Rat pyrroline-5-carboxylate reductase family, member 2 (Pycr2), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001012208" in other vectors (3)
USD 198.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Pycr2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR214930 representing NM_001012208
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGTGGGTTTCATCGGCGCGGGCCAGCTGGCCTGTGCTCTGGCGCGGGGCTTCACGGCCGCGGGCG TCCTGTCGGCTCACAAGATAATAGCCAGCTCCCCGGAAATGGACCTGCCCACGGTGTCTGCGCTCAGGAA GATGGGTGTGAATCTGACCCGAAGCAATAAGGACACGGTGCGGCACAGTGACGTCCTGTTCCTGGCTGTG AAGCCCCACATCATCCCCTTCATCCTGGATGAGATCGGGGCTGACGTGCAGGAGAGGCACATCGTGGTCT CCTGTGCGGCTGGCGTCACCATCAGTTCTGTGGAGAAGAAGCTGATGGCTTTCCAGCCAGCCCCCAAAGT GATCCGCTGCATGACCAACACGCCTGTGGTGGTCCGAGAGGGGGCCACTGTGTACGCCACAGGCACCCAT GCTCTGGTGGAAGATGGGAAGCTTCTGGAGCAGCTGATGAGCAGTGTGGGCTTCTGCACAGAGGTGGAGG AAGACCTCATTGATGCTATCACAGGGCTCAGCGGCAGCGGGCCCGCCTACGCGTTTATGGCCCTGGATGC GCTGGCAGACGGCGGGGTGAAGATGGGCGTGCCGCGGCGCCTGGCAGTCCGACTGGGAGCTCAGGCGTTG CTGGGAGCAGCTAAGATGCTGCTGGATTCAGAGGACCACCCGGGCCAGCTCAAGGACAACGTCTGCTCCC CTGGGGGGGCCACCATCCATGCCCTGCACTTCCTGGAGAGTGGGGGCTTCCGCTCTCTGCTCATCAATGC AGTTGAGGCCTCCTGTATTCGAACAAGAGAGCTGCAGTCCATGGCTGACCAAGAAAAGGTGTCCCCTGCC GCCCTTAAGAAGACCCTCCTGGACAGAGTGAAGCTGGAGTCACCCACGGTGTCCACGCTGGCCCCACCCA GCTCAGGGAAACTCCTTACAAGAAACCCTGCCCAGGGAAGCAAGAGGGAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR214930 representing NM_001012208
Red=Cloning site Green=Tags(s) MSVGFIGAGQLACALARGFTAAGVLSAHKIIASSPEMDLPTVSALRKMGVNLTRSNKDTVRHSDVLFLAV KPHIIPFILDEIGADVQERHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVREGATVYATGTH ALVEDGKLLEQLMSSVGFCTEVEEDLIDAITGLSGSGPAYAFMALDALADGGVKMGVPRRLAVRLGAQAL LGAAKMLLDSEDHPGQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKVSPA ALKKTLLDRVKLESPTVSTLAPPSSGKLLTRNPAQGSKRE SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001012208 |
ORF Size | 960 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001012208.1, NP_001012208.1 |
RefSeq Size | 1582 bp |
RefSeq ORF | 963 bp |
Locus ID | 364064 |
UniProt ID | Q6AY23 |
Cytogenetics | 13q26 |
MW | 33.7 kDa |
Gene Summary | Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH (By similarity). Involved in cellular response to oxidative stress (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN214930 | Pycr2 (untagged ORF) - Rat pyrroline-5-carboxylate reductase family, member 2 (Pycr2), (10 ug) |
USD 330.00 |
|
RR214930L3 | Lenti ORF clone of Pycr2 (Myc-DDK-tagged ORF) - Rat pyrroline-5-carboxylate reductase family, member 2 (Pycr2), (10 ug) |
USD 630.00 |
|
RR214930L4 | Lenti ORF clone of Pycr2 (mGFP-tagged ORF) - Rat pyrroline-5-carboxylate reductase family, member 2 (Pycr2), (10 ug) |
USD 630.00 |
{0} Product Review(s)
Be the first one to submit a review