Pycr2 (NM_001012208) Rat Tagged ORF Clone

CAT#: RR214930

Pycr2 (Myc-DDK-tagged ORF) - Rat pyrroline-5-carboxylate reductase family, member 2 (Pycr2), (10 ug)

Product Images

Circular map for RR214930

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pycr2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214930 representing NM_001012208
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGTGGGTTTCATCGGCGCGGGCCAGCTGGCCTGTGCTCTGGCGCGGGGCTTCACGGCCGCGGGCG
  TCCTGTCGGCTCACAAGATAATAGCCAGCTCCCCGGAAATGGACCTGCCCACGGTGTCTGCGCTCAGGAA
  GATGGGTGTGAATCTGACCCGAAGCAATAAGGACACGGTGCGGCACAGTGACGTCCTGTTCCTGGCTGTG
  AAGCCCCACATCATCCCCTTCATCCTGGATGAGATCGGGGCTGACGTGCAGGAGAGGCACATCGTGGTCT
  CCTGTGCGGCTGGCGTCACCATCAGTTCTGTGGAGAAGAAGCTGATGGCTTTCCAGCCAGCCCCCAAAGT
  GATCCGCTGCATGACCAACACGCCTGTGGTGGTCCGAGAGGGGGCCACTGTGTACGCCACAGGCACCCAT
  GCTCTGGTGGAAGATGGGAAGCTTCTGGAGCAGCTGATGAGCAGTGTGGGCTTCTGCACAGAGGTGGAGG
  AAGACCTCATTGATGCTATCACAGGGCTCAGCGGCAGCGGGCCCGCCTACGCGTTTATGGCCCTGGATGC
  GCTGGCAGACGGCGGGGTGAAGATGGGCGTGCCGCGGCGCCTGGCAGTCCGACTGGGAGCTCAGGCGTTG
  CTGGGAGCAGCTAAGATGCTGCTGGATTCAGAGGACCACCCGGGCCAGCTCAAGGACAACGTCTGCTCCC
  CTGGGGGGGCCACCATCCATGCCCTGCACTTCCTGGAGAGTGGGGGCTTCCGCTCTCTGCTCATCAATGC
  AGTTGAGGCCTCCTGTATTCGAACAAGAGAGCTGCAGTCCATGGCTGACCAAGAAAAGGTGTCCCCTGCC
  GCCCTTAAGAAGACCCTCCTGGACAGAGTGAAGCTGGAGTCACCCACGGTGTCCACGCTGGCCCCACCCA
  GCTCAGGGAAACTCCTTACAAGAAACCCTGCCCAGGGAAGCAAGAGGGAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214930 representing NM_001012208
  Red=Cloning site Green=Tags(s)
  
  MSVGFIGAGQLACALARGFTAAGVLSAHKIIASSPEMDLPTVSALRKMGVNLTRSNKDTVRHSDVLFLAV
  KPHIIPFILDEIGADVQERHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVREGATVYATGTH
  ALVEDGKLLEQLMSSVGFCTEVEEDLIDAITGLSGSGPAYAFMALDALADGGVKMGVPRRLAVRLGAQAL
  LGAAKMLLDSEDHPGQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKVSPA
  ALKKTLLDRVKLESPTVSTLAPPSSGKLLTRNPAQGSKRE
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012208
• ORF Size: 960 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012208.1, NP_001012208.1
• RefSeq Size: 1582 bp
• RefSeq ORF: 963 bp
• Locus ID: 364064
• UniProt ID: Q6AY23
• Cytogenetics: 13q26
• MW: 33.7 kDa
• Gene Summary: Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH (By similarity). Involved in cellular response to oxidative stress (By similarity).[UniProtKB/Swiss-Prot Function]