Znrf2 (NM_001108628) Rat Tagged ORF Clone

CAT#: RR214668

Znrf2 (Myc-DDK-tagged ORF) - Rat zinc and ring finger 1 (Znrf1), (10 ug)

Product Images

Circular map for RR214668

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Znrf2
• Synonyms: Znrf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214668 representing NM_001108628
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCCAAGCAGAGCGGCCCCGCCGCCAACGGCCGCACCCGCGCCTACTCGGGCTCGGACCTGCCCT
  CCGGGACCAGCGGCGGCGGCGGGGCGGACGGCGCTCGGGCGGCTCGGTTCGCGGCCCCTGTCCCCGGCGC
  GCAGCAGCCCAGCGCCTCGGCCAGCACGGCGGAGGATGCTGCTGCTGCGGCGGCGGCACGGCACAGCGGC
  GGCCTCGGCCCCGGCAGCGCCCGTAGCCGCTCGCTCGGTGGGGCGGTGGGCAGCGCGGCGAGCGGCCGCG
  CGGCACAGTCGGCCTTCAGCATTCCCAGCAGTGGCGGCGGCAGCGGTGGCCCGTACGGCTCACAGGACTC
  GGTGCACAGCAGCCCAGAGGACGGTGTCGGCTCTAGGGACCGGGACCGGCCAGCGGGCGGGGGCCCTGGC
  GGGCCGCGTCTGGTGATCGGCTCGCTCCCGGCCCACCTCTCCCCGCACTTGTTTGGAGGATTTAAGTGCC
  CGGTATGCTCAAAATTTGTACCCTCAGATGAAATGGATTTGCATCTTGTGATGTGTTTAACAAAGCCAAG
  AATAACGTATAATGAGGATGTGCTGAGTAAAGATGCGGGGGAATGTGCAATATGCCTTGAAGAATTGCAG
  CAGGGAGACACCATAGCACGACTGCCTTGCCTGTGTATATATCATAAAGGCTGCATAGATGAATGGTTTG
  AAGTAAATAGATCTTGTCCAGAGCACCCTTCGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214668 representing NM_001108628
  Red=Cloning site Green=Tags(s)
  
  MGAKQSGPAANGRTRAYSGSDLPSGTSGGGGADGARAARFAAPVPGAQQPSASASTAEDAAAAAAARHSG
  GLGPGSARSRSLGGAVGSAASGRAAQSAFSIPSSGGGSGGPYGSQDSVHSSPEDGVGSRDRDRPAGGGPG
  GPRLVIGSLPAHLSPHLFGGFKCPVCSKFVPSDEMDLHLVMCLTKPRITYNEDVLSKDAGECAICLEELQ
  QGDTIARLPCLCIYHKGCIDEWFEVNRSCPEHPSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108628
• ORF Size: 735 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108628.1, NP_001102098.1
• RefSeq Size: 5774 bp
• RefSeq ORF: 738 bp
• Locus ID: 362367
• Cytogenetics: 4q24
• MW: 24.5 kDa