Acy1 (NM_001005383) Rat Tagged ORF Clone

CAT#: RR214639

Acy1 (Myc-DDK-tagged ORF) - Rat aminoacylase 1 (Acy1), (10 ug)

Product Images

Circular map for RR214639

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acy1
• Synonyms: Acy1a; Acy1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214639 representing NM_001005383
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAGCAAGGTTCCCGAGGAGGAGCACCCATCGGTGACGCTCTTCCGCCAGTACCTGCGCATCTGCA
  CTGTGCAACCCAATCCTGACTATGGGAGTGCGGTCACCTTCCTTGAGGAGAGAGCCCGCCAGCTGGGTCT
  GAGCTGTCAGAAAATAGAGGTGGCGCCTGGCTATGTGATCACTGTGCTGACTTGGCCAGGCACCAACCCG
  TTGCTTCATTCCATCTTGCTAAACTCCCACACAGATGTGGTACCTGTCTTCAAGGAGCATTGGCATCACG
  ACCCCTTTGAAGCCTTCAAGGATTCCGAGGGCTACATCTATGCTAGGGGCGCCCAGGACATGAAGAGTGT
  CAGCATCCAGTACCTGGAGGCCGTGAGAAGGCTGAAGAGCGAGAGCCACCGGTTCCCCAGAACCATCCAC
  ATGACATTTGTGCCTGACGAGGAGGTCGGAGGTCACAAGGGGATGGAGTTGTTTGTGAAGAGGCCTGAGT
  TCCAGGCTCTGCGGGCAGGCTTTGCTCTGGATGAGGGCCTGGCCAACCCCACTGATGCCTTCACCGTTTT
  TTATAGTGAACGGAGTCCTTGGTGGATACGGGTTACCAGCACTGGGAAGCCAGGCCATGCCTCACGCTTC
  ATTGAAGATACAGCAGCTGAGAAGTTGCATAAGGTTGTAAACTCCATCTTGGCATTCCGGGAGAAGGAGA
  GGCAGAGGCTGCAGGCGAACCCTCACCTGAAGGAAGGGGCCGTGACTTCGGTGAATCTGACTAAGCTCGA
  GGGTGGTGTGGCCTATAATGTGGTACCTGCTACCATGAGTGCCTGTTTTGACTTCCGAGTGGCGCCAGAT
  GTAGACATGAAGGCCTTCGAGAAGCAGCTGCAGAGCTGGTGCCAGGAAGCGGGAGAGGGGGTCACCTTTG
  AGTTTGCTCAGAAGTTTACAGAGCCTCGAATGACACCCACTGATGATACGGACCCCTGGTGGGCAGCTTT
  CAGCGGGGCCTGCAAGGAAATGAACCTCACTCTGGAGCCGGAGATCTTTCCTGCTGCCACTGACAGCCGT
  TATATCCGAGCGGTGGGGATCCCAGCTCTGGGCTTCTCGCCCATGAACCGCACACCCGTGTTACTACATG
  ACCATAACGAACGACTGCACGAGGCTGTGTTTCTACGTGGAGTTGACATATATACACGCCTAGTTGCTGC
  ACTTGCTAGTGTGCCCGCTCTGCCTGGTGAAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214639 representing NM_001005383
  Red=Cloning site Green=Tags(s)
  
  MTSKVPEEEHPSVTLFRQYLRICTVQPNPDYGSAVTFLEERARQLGLSCQKIEVAPGYVITVLTWPGTNP
  LLHSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGAQDMKSVSIQYLEAVRRLKSESHRFPRTIH
  MTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFTVFYSERSPWWIRVTSTGKPGHASRF
  IEDTAAEKLHKVVNSILAFREKERQRLQANPHLKEGAVTSVNLTKLEGGVAYNVVPATMSACFDFRVAPD
  VDMKAFEKQLQSWCQEAGEGVTFEFAQKFTEPRMTPTDDTDPWWAAFSGACKEMNLTLEPEIFPAATDSR
  YIRAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALASVPALPGES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005383
• ORF Size: 1224 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005383.1, NP_001005383.1
• RefSeq Size: 1356 bp
• RefSeq ORF: 1227 bp
• Locus ID: 300981
• UniProt ID: Q6AYS7
• Cytogenetics: 8q32
• MW: 45.9 kDa
• Gene Summary: enzyme that displays aminoacylase and/or metallopeptidase functions; may be involved in amino acid metabolism [RGD, Feb 2006]