Vps35 (NM_001105718) Rat Tagged ORF Clone

CAT#: RR214633

Vps35 (Myc-DDK-tagged ORF) - Rat vacuolar protein sorting 35 homolog (S. cerevisiae) (Vps35), (10 ug)

Product Images

Circular map for RR214633

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vps35
• Synonyms: Mem3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214633 representing NM_001105718
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTACAACACAGCAATCACCCCAGGATGAGCAAGAAAAACTCTTGGATGAAGCCATCCAGGCTGTGA
  AAGTTCAATCATTCCAGATGAAAAGATGCCTGGACAAAAACAAGCTGATGGACGCTCTGAAACATGCCTC
  GAATATGCTTGGAGAACTCCGGACTTCTATGTTATCACCAAAGAGTTACTATGAACTTTATATGGCTATT
  TCTGATGAGCTGCACTACTTGGAGGTCTATTTGACTGATGAATTTGCTAAAGGAAGAAAGGTGGCAGATC
  TCTATGAACTTGTACAGTATGCTGGAAACATTATTCCAAGGCTTTATCTCTTGATCACAGTTGGAGTTGT
  GTATGTCAAGTCATTTCCTCAGTCCAGGAAAGATATTCTGAAAGATTTGGTAGAAATGTGCCGTGGCGTG
  CAGCATCCACTAAGGGGTTTGTTTCTTCGAAATTATCTTCTTCAGTGTACTAGGAACATTTTACCTGATG
  AAGGAGAGCCAACAGATGAAGAAACAACTGGTGATATCAGTGATTCCATGGATTTTGTACTACTCAACTT
  TGCAGAAATGAATAAGCTCTGGGTGCGGATGCAGCATCAAGGACATAGTCGAGATAGAGAAAAAAGAGAA
  CGAGAGAGACAAGAACTGAGAATTTTAGTGGGAACTAATTTGGTGCGCCTTAGTCAGTTGGAAGGTGTAA
  ATGTGGAACGTTACAAACAGATTGTTTTAACAGGCATTTTGGAGCAAGTTGTGAATTGTAGAGATGCTTT
  GGCTCAAGAGTATCTCATGGAGTGCATCATTCAGGTTTTTCCTGATGAATTCCATCTCCAGACTTTGAAT
  CCTTTTCTTAGAGCTTGTGCTGAGTTACACCAAAATGTAAATGTGAAAAACATAATCATTGCTTTAATTG
  ACAGATTAGCTTTATTTGCTCATCGTGAAGATGGACCTGGAATTCCAGCTGAGATTAAACTTTTTGACAT
  ATTTTCACAACAGGTGGCTACAGTGATACAGTCCAGACAAGACATGCCATCAGAGGACGTTGTATCTTTA
  CAAGTCTCTCTCATTAATCTTGCTATGAAATGTTACCCTGATCGTGTGGACTATGTTGATAAAGTTCTAG
  AAACAACAGTGGAGATATTCAATAAACTTAACCTTGAACATATTGCTACCAGTAGTGCAGTTTCAAAGGA
  GCTTACCAGACTTTTGAAGATTCCAGTTGATACTTACAACAATATCTTAACAGTCTTAAAGTTAAAGCAT
  TTCCACCCACTTTTTGAGTACTTTGACTACGAGTCCAGAAAGAGCATGAGCTGTTATGTGCTTAGTAATG
  TTCTGGATTATAACACAGAAATCGTCTCTCAGGACCAGGTAGATTCCATAATGAATTTGGTGTCCACGTT
  GATTCAGGATCAGCCAGACCAGCCTGTAGAAGACCCTGACCCAGAAGACTTTGCTGATGAACAGAGCCTT
  GTTGGCCGATTTATTCACCTTCTGCGTTCTGAAGACCCTGATCAGCAGTATTTGATTTTGAATACAGCAC
  GAAAACATTTTGGGGCTGGTGGAAATCAGCGGATTCGCTTCACACTGCCACCTTTGGTATTTGCAGCTTA
  TCAGTTGGCTTTTCGGTACAAAGAGAATTCTCAAATGGATGACAAATGGGAAAAGAAATGCCAGAAGATT
  TTTTCATTTGCCCATCAGACTATCAGTGCTTTGATTAAAGCCGAGCTGGCTGAGTTACCACTGAGACTTT
  TTCTTCAAGGAGCATTAGCTGCTGGGGAGATTGGCTTCGAAAATCATGAAACAGTAGCATATGAATTTAT
  GTCCCAGGCGTTTTCTCTATATGAGGATGAACTCAGTGACTCCAAAGCACAGCTGGCTGCTATCACTTTG
  ATCATTGGTACTTTTGAGAGGATGAAATGCTTCAGTGAAGAGAATCATGAGCCCTTGAGAACTCAGTGTG
  CACTTGCTGCATCAAAACTTCTGAAAAAACCGGACCAAGGCCGAGCTGTGAGCACATGTGCACATCTCTT
  TTGGTCTGGCCGAAACACAGACAAAAATGGGGAAGAGCTTCATGGAGGTAAAAGGGTCATGGAGTGCCTA
  AAGAAGGCACTAAAAATAGCAAATCAGTGCATGGACCCCTCTCTACAAGTTCAGCTCTTTATAGAGATTC
  TGAACAGGTACATCTATTTCTATGAAAAAGAAAATGATGCGGTAACAATTCAGGTCTTGAACCAACTTAT
  TCAAAAAATTCGAGAAGATCTCCCAAACCTTGAGTCCAGTGAAGAAACAGAGCAAATTAACAAGCATTTT
  CACAACACGTTGGAGCACTTGCGCTCAAGACGGGAATCACCAGAGTCTGAGGGGCCAATCTATGAAGGTC
  TCATCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214633 representing NM_001105718
  Red=Cloning site Green=Tags(s)
  
  MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAI
  SDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGV
  QHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRE
  RERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLN
  PFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPAEIKLFDIFSQQVATVIQSRQDMPSEDVVSL
  QVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKH
  FHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSL
  VGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSQMDDKWEKKCQKI
  FSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDELSDSKAQLAAITL
  IIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECL
  KKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHF
  HNTLEHLRSRRESPESEGPIYEGLIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105718
• ORF Size: 2388 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105718.2, NP_001099188.2
• RefSeq Size: 3196 bp
• RefSeq ORF: 2391 bp
• Locus ID: 25479
• Cytogenetics: 19q11
• MW: 91.7 kDa
• Gene Summary: mouse homolog is a hydrophilic membrane protein having a direct role in the retrieval of cargo proteins [RGD, Feb 2006]