Ube2q1 (NM_001106448) Rat Tagged ORF Clone

CAT#: RR214565

Ube2q1 (Myc-DDK-tagged ORF) - Rat ubiquitin-conjugating enzyme E2Q family member 1 (Ube2q1), (10 ug)

Product Images

Circular map for RR214565

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ube2q1
• Synonyms: Ube2q
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214565 representing NM_001106448
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCAGCCGCAGCCGCAGGGGCAGCAGCAGCCGGGGCCGGGGCAGCAACTGGGGGTCCAGGGGGCGG
  CGCCGGGGGCCGGGGGCGGCCCGGGAGGGGGCCCGGGGCCAGGGCCCTGCCTGAGGCGGGAGCTGAAGCT
  GCTCGAGTCCATCTTCCACCGCGGCCACGAGCGCTTCCGCATTGCCAGCGCCTGCCTGGACGAGCTGAGC
  TGCGAGTTCCTGCTGGCCGGAGCCGGAGGGGCCGGGGCCGGGGCTGCGCCCGGACCCCATCTCCCCTCCC
  GGGGGTCGGTGCCGGGGGACCCCGTCCGCATCCACTGCAACATCACGGAATCATACCCTGCTGTGCCCCC
  CATCTGGTCGGTGGAGTCTGATGATCCTAACTTGGCTGCTGTCTTGGAGAGGCTGGTGGACATAAAGAAA
  GGGAATACTTTGCTCTTGCAGCATCTGAAGAGGATCATCTCTGACCTGTGTAAACTCTACAACCTCCCTC
  AGCATCCTGATGTGGAAATGCTGGATCAGCCCTTGCCAGCAGAGCAGTGCACGCAGGAAGAAGTATCTTC
  AGAAGACGAAGACGAAGAGATGCCCGAGGATACAGAAGACCTAGATCACTATGAGATGAAAGAGGAGGAG
  CCAGCGGAGGGCAAGAAGTCTGAAGATGATGGCATCGGAAAAGAAAACCTGGCCATCCTAGAGAAAATTA
  AAAAGAACCAGAGGCAAGATTACTTAAATGGTGCAGTGTCTGGCTCGGTGCAGGCCACTGACCGGCTGAT
  GAAGGAGCTCAGGGATATATACCGATCACAGAGTTTCAAAGGCGGAAACTATGCAGTCGAACTCGTGAAT
  GACAGTCTGTATGACTGGAATGTCAAACTCCTCAAAGTTGACCAGGACAGCGCTTTGCACAATGATCTTC
  AGATCCTAAAGGAGAAGGAAGGAGCAGACTTCATCCTACTCAACTTCTCCTTTAAAGATAACTTCCCCTT
  TGACCCACCGTTCGTCAGGGTTGTGTCTCCAGTCCTCTCTGGAGGGTATGTTCTGGGCGGAGGTGCCATC
  TGCATGGAGCTGCTCACCAAGCAGGGCTGGAGCAGCGCCTACTCCATAGAGTCCGTGATCATGCAGATCA
  GCGCCACGCTGGTGAAGGGGAAGGCACGGGTGCAGTTCGGAGCCAACAAATCTCAGTATAGTCTGACAAG
  AGCACAGCAGTCCTACAAGTCCTTGGTGCAGATCCACGAAAAAAACGGCTGGTACACACCCCCGAAGGAA
  GATGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214565 representing NM_001106448
  Red=Cloning site Green=Tags(s)
  
  MQQPQPQGQQQPGPGQQLGVQGAAPGAGGGPGGGPGPGPCLRRELKLLESIFHRGHERFRIASACLDELS
  CEFLLAGAGGAGAGAAPGPHLPSRGSVPGDPVRIHCNITESYPAVPPIWSVESDDPNLAAVLERLVDIKK
  GNTLLLQHLKRIISDLCKLYNLPQHPDVEMLDQPLPAEQCTQEEVSSEDEDEEMPEDTEDLDHYEMKEEE
  PAEGKKSEDDGIGKENLAILEKIKKNQRQDYLNGAVSGSVQATDRLMKELRDIYRSQSFKGGNYAVELVN
  DSLYDWNVKLLKVDQDSALHNDLQILKEKEGADFILLNFSFKDNFPFDPPFVRVVSPVLSGGYVLGGGAI
  CMELLTKQGWSSAYSIESVIMQISATLVKGKARVQFGANKSQYSLTRAQQSYKSLVQIHEKNGWYTPPKE
  DG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106448
• ORF Size: 1266 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106448.2, NP_001099918.1
• RefSeq Size: 1832 bp
• RefSeq ORF: 1269 bp
• Locus ID: 295252
• Cytogenetics: 2q34
• MW: 46.2 kDa