Pou6f2 (NM_001101002) Rat Tagged ORF Clone

CAT#: RR214306

Pou6f2 (Myc-DDK-tagged ORF) - Rat similar to POU domain, class 6, transcription factor 2 (LOC681092), (10 ug)

Product Images

Circular map for RR214306

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pou6f2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214306 representing NM_001101002
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAGCTGGACAAGTCAGTAAGCCCTTGCTGTCAGTGCGGAGTGAAATGAATGCGGAGTTGAGAGGTG
  AGGACAAGGCTGCTACTTCAGACAGCGAGCTGAATGAGCCCCTGCTTGCGCCCGTGGAATCAAATGACAG
  CGAGGACACTCCCAGCAAGCTCTTCGGGGCCAGAGGAAACACAGTCCTACCAGAACCAGGAACTCCTGAC
  CAGCACCAGGCCAGTCAGACCCATCCCTCCTTTCCAGTTGGACCACAGCCTCTTCTAACGGCACAGCAGT
  TAGCCTCTGCTGTGGCCGGGGTGATGCCGGGAGGCCCCCCAGCCCTCAATCAGCCTATCCTCATCCCCTT
  CAACATGGCGGGACAACTAGGTGGTCAGCAAGGCCTGGTCCTCACACTGCCCACCGCGAATCTCACAAAC
  ATCCAAGGGCTGGTAGCGGCAGCTGCAGCTGGAGGCATTATGACTCTGCCATTGCAAAATTTACAAGCTA
  CCTCATCCCTGAACTCCCAGCTGCAACAGCTCCAGCAACTCCAGCTCCAGCAGCAACAGCAGCAGCAACA
  ACCACCTCCTCCACCAACCAGCCAGCACCCACAGCCAGCCTCACAGGCGCCCGCACAGTCTCAGCAGCCG
  CAGTCCCAGCCCACCCCGCCTCACCAGCCACCACCTGCTTCACAGCAGTTGCCAGCTGCCCCAGCTCAGC
  TGCCGCAGGCTACTCAGCCTCAACAGCATCAACCTCATTCCCATCCCCAGAACCAGAACCAGTCGTCTCC
  AACCCAGCAGAGCTCAAGCCCCCCTCAGAAACCCACCCCATCTCCAGGAGGACATAGCCTTCCGTCACCA
  CTCACACCGCCTAACCCTCTACAGATTATTGGGACCATTCCACTGATGCCTAACCCAGGGCCATCGAGTC
  AAGCAGCAAGTGGCACTCAGGGCCTTCAAGTACAGCCTATCACACCCCAGCTCCTAACCAATGCCCAGGG
  CCAAATCATTGCCACGGTCATCGGAAACCAGATCTTGCCTGTGATCAATACCCAGGGTATTACCCTGTCC
  CCCATCAAGCCAGGCCAGCAGCTTCACCAATCTTCCCAGACATCAGTTGGTCAAGCAGGCACCCAAGGCA
  ACCTCCTGCACCTGGCTCATGGCCAAGCATCCACCTCTCAAAGTCCGGTGCGACAGGCTTCCTCCTCCTC
  CTCTTCATCCTCCTCTTCTTCAGCTTTGAGCGTGGGCCAGTTAGTCAGCACTTACAGAATGCATTACTAC
  TTGACAGTGCCTTTGGAGCTGTTTAAATGGCAGCATAGAGTGCAGAAGGGAACAGAGTTTTGGAATACAG
  ACTCCTCTTCATGTGCTGACACTGTGGGCCCCTCAAATAGAGCGACCCATCCTTTTGCAAGAAACTTGAC
  CGGCACTACGGTGCCCCAAGATATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214306 representing NM_001101002
  Red=Cloning site Green=Tags(s)
  
  MIAGQVSKPLLSVRSEMNAELRGEDKAATSDSELNEPLLAPVESNDSEDTPSKLFGARGNTVLPEPGTPD
  QHQASQTHPSFPVGPQPLLTAQQLASAVAGVMPGGPPALNQPILIPFNMAGQLGGQQGLVLTLPTANLTN
  IQGLVAAAAAGGIMTLPLQNLQATSSLNSQLQQLQQLQLQQQQQQQQPPPPPTSQHPQPASQAPAQSQQP
  QSQPTPPHQPPPASQQLPAAPAQLPQATQPQQHQPHSHPQNQNQSSPTQQSSSPPQKPTPSPGGHSLPSP
  LTPPNPLQIIGTIPLMPNPGPSSQAASGTQGLQVQPITPQLLTNAQGQIIATVIGNQILPVINTQGITLS
  PIKPGQQLHQSSQTSVGQAGTQGNLLHLAHGQASTSQSPVRQASSSSSSSSSSSALSVGQLVSTYRMHYY
  LTVPLELFKWQHRVQKGTEFWNTDSSSCADTVGPSNRATHPFARNLTGTTVPQDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001101002
• ORF Size: 1425 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001101002.1, NP_001094472.1
• RefSeq Size: 2445 bp
• RefSeq ORF: 1428 bp
• Locus ID: 681092
• Cytogenetics: 17q11
• MW: 49.7 kDa