Egr3 (NM_017086) Rat Tagged ORF Clone

CAT#: RR214264

Egr3 (Myc-DDK-tagged ORF) - Rat early growth response 3 (Egr3), (10 ug)

Product Images

Circular map for RR214264

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Egr3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214264 representing NM_017086
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGGCAAACTCGCCGAGAAGCTGCCGGTGACCATGAGCAGTTTGCTAAATCAATTGCCTGACAATC
  TGTACCCCGAGGAGATCCCCAGCGCGCTCAACCTCTTCTCCGGCAGCAGTGACTCGGTAGCCCATTACAC
  TCAGATGGCTACAGAGAATGTGATGGACATCGGTCTGACCAACGAGAAGCCCAATCCGGAACTCTCTTAT
  TCGGGCTCTTTCCAGCCAGCCCCAGGCAACAAGACCGTGACCTACTTGGGAAAGTTCGCTTTCGACTCTC
  CTTCCAACTGGTGCCAGGACAACATCATTAGCCTCATGAGTGCCGGCATCTTGGGGGTGCCCCCGGCCTC
  AGGGGCGCTCAGTACGCAGACGACCACGGCTAGCATGGTGCAGCCTCCTCAGGGAGACGTGGAGGCCATG
  TATCCGGCGCTGCCCCCCTATTCCAACTGCGGCGACCTCTACTCGGAGCCGGTGTCTTTCCACGACCCCC
  AGGGGAACCCAGGGCTCGCCTATTCCCCCCAGGATTACCAATCGGCCAAGCCGGCCTTGGACAGCAATCT
  TTTCCCCATGATTCCTGACTACAACCTGTATCACCACCCCAACGACATGGGCTCCATTCCAGAACACAAG
  CCCTTCCAGGGCATGGACCCCATTCGGGTCAACCCACCCCCTATTACCCCTCTGGAGACCATCAAGGCCT
  TCAAAGACAAGCAGATCCACCCGGGCTTCGGCAGCTTGCCCCAACCGCCGCTTACTCTCAAGCCCATCCG
  GCCCCGCAAGTACCCCAATCGGCCTAGCAAGACCCCACTCCACGAGCGGCCCCACGCGTGTCCCGCGGAA
  GGCTGTGACCGGCGTTTCAGCCGCTCGGACGAGCTGACCCGGCACCTGCGCATCCACACGGGCCACAAGC
  CCTTCCAGTGTCGGATCTGCATGCGCAGCTTCAGCCGCAGCGACCACCTCACTACTCACATCCGCACGCA
  CACCGGGGAGAAGCCCTTTGCCTGTGAGTTCTGTGGGCGCAAGTTTGCGCGCAGCGACGAGCGCAAGCGC
  CACGCCAAGATCCACCTCAAGCAAAAGGAGAAGAAGTCGGAGAAAGGGGGTGCGCCCTCTGCATCCTCGG
  CGCCCACCGTGTCCCTGGCCCCTGTGGTCACCACCTGCGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214264 representing NM_017086
  Red=Cloning site Green=Tags(s)
  
  MTGKLAEKLPVTMSSLLNQLPDNLYPEEIPSALNLFSGSSDSVAHYTQMATENVMDIGLTNEKPNPELSY
  SGSFQPAPGNKTVTYLGKFAFDSPSNWCQDNIISLMSAGILGVPPASGALSTQTTTASMVQPPQGDVEAM
  YPALPPYSNCGDLYSEPVSFHDPQGNPGLAYSPQDYQSAKPALDSNLFPMIPDYNLYHHPNDMGSIPEHK
  PFQGMDPIRVNPPPITPLETIKAFKDKQIHPGFGSLPQPPLTLKPIRPRKYPNRPSKTPLHERPHACPAE
  GCDRRFSRSDELTRHLRIHTGHKPFQCRICMRSFSRSDHLTTHIRTHTGEKPFACEFCGRKFARSDERKR
  HAKIHLKQKEKKSEKGGAPSASSAPTVSLAPVVTTCA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017086
• ORF Size: 1161 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017086.1, NP_058782.1
• RefSeq Size: 1164 bp
• RefSeq ORF: 1164 bp
• Locus ID: 25148
• UniProt ID: P43301
• Cytogenetics: 15p11
• MW: 42.6 kDa
• Gene Summary: zinc finger transcription factor; may mediate long-term, neuroplastic responses [RGD, Feb 2006]