Mapk3 (NM_017347) Rat Tagged ORF Clone

CAT#: RR214231

Mapk3 (Myc-DDK-tagged ORF) - Rat mitogen activated protein kinase 3 (Mapk3), (10 ug)

Product Images

Circular map for RR214231

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mapk3
• Synonyms: Erk-1; ERK1; ERT2; Esrk1; MAPK1; MNK1; p44; p44erk1; p44mapk; Prkm3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214231 representing NM_017347
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGGCGGCTCCGGGGGGCGGGGGCGGGGAGCCCAGGGGAACTGCTGGGGTCGTCCCGG
  TGGTCCCCGGGGAGGTGGAGGTGGTGAAGGGGCAGCCATTCGACGTGGGCCCACGCTACACGCAGCTGCA
  GTACATCGGCGAGGGCGCGTACGGCATGGTCAGCTCAGCATATGACCACGTGCGCAAGACCAGAGTGGCT
  ATCAAGAAGATCAGCCCCTTCGAGCATCAAACCTACTGTCAGCGCACGCTGAGAGAAATCCAGATCTTGC
  TCGGATTCCGCCATGAGAATGTCATAGGCATCCGAGACATCCTCAGAGCACCCACCCTGGAAGCCATGAG
  AGATGTTTACATTGTTCAGGACCTCATGGAGACGGACCTGTACAAGCTGCTAAAGAGCCAGCAGCTGAGC
  AATGACCACATCTGCTACTTCCTCTACCAGATCCTCCGGGGCCTCAAGTACATACACTCGGCCAATGTGC
  TGCACCGGGACCTGAAGCCCTCCAATCTGCTTATCAACACCACCTGCGACCTTAAGATCTGTGATTTTGG
  CCTTGCCCGGATTGCTGACCCTGAGCACGACCACACTGGCTTTCTGACCGAGTATGTGGCCACACGCTGG
  TACCGAGCCCCAGAGATCATGCTTAACTCCAAGGGCTACACCAAATCCATTGACATCTGGTCTGTGGGCT
  GCATTCTGGCTGAGATGCTCTCCAACCGGCCTATCTTCCCCGGCAAGCACTACCTGGACCAGCTCAACCA
  CATTCTAGGTATACTGGGTTCCCCATCCCAAGAGGACCTAAATTGTATCATTAACATGAAGGCCCGAAAC
  TACCTACAGTCTCTGCCCTCTAAAACCAAGGTGGCTTGGGCCAAGCTTTTTCCCAAATCTGACTCCAAAG
  CTCTTGACCTGCTGGACCGGATGTTAACCTTTAACCCAAACAAGCGCATCACAGTAGAGGAAGCACTGGC
  TCACCCTTACCTGGAACAGTACTATGATCCGACAGATGAACCAGTGGCTGAGGAGCCATTCACCTTTGAC
  ATGGAGCTGGATGATCTCCCCAAGGAGCGGCTGAAGGAGCTGATCTTCCAAGAGACAGCCCGCTTCCAGC
  CAGGGGCACCAGAGGCCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214231 representing NM_017347
  Red=Cloning site Green=Tags(s)
  
  MAAAAAAPGGGGGEPRGTAGVVPVVPGEVEVVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVA
  IKKISPFEHQTYCQRTLREIQILLGFRHENVIGIRDILRAPTLEAMRDVYIVQDLMETDLYKLLKSQQLS
  NDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRW
  YRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARN
  YLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFD
  MELDDLPKERLKELIFQETARFQPGAPEAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017347
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017347.2, NP_059043.1
• RefSeq Size: 1875 bp
• RefSeq ORF: 1143 bp
• Locus ID: 50689
• UniProt ID: P21708
• Cytogenetics: 1q36
• MW: 43 kDa
• Gene Summary: kinase involved in intracellular signalling; component of Mapk signalling pathway [RGD, Feb 2006]