Gbp2 (NM_133624) Rat Tagged ORF Clone

CAT#: RR214194

Gbp2 (Myc-DDK-tagged ORF) - Rat guanylate binding protein 2 (Gbp2), (10 ug)

Product Images

Circular map for RR214194

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gbp2
• Synonyms: MGC93208
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214194 representing NM_133624
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATGGCCTCGGAGATCCACATGTTACAACCCATGTGCCTCATTGAGAACACTGAGGCTCACCTAG
  TGATCAACCAGGAGGCTCTGAGGATTTTGTCTGCCATCAACCAGCCTGTGGTGGTGGTGGCAATCGTGGG
  CCTCTACCGCACAGGCAAATCCTACCTGATGAACAAATTGGCTGGGAAGAGAACAGGCTTCTCCCTGGGC
  TCCACTGTGCAGTCTCACACCAAGGGCATCTGGATGTGGTGTGTGCCTCACCCCAAGAAGGCAGGGCAAA
  CCCTAGTTCTGCTTGACACCGAGGGCCTTGAAGATGTTGAGAAGGGAGACAACCAGAACGACTGCTGGAT
  CTTTGCCTTGGCAGTCCTCCTCAGCAGCACCTTCGTCTACAACAGCATGGGAACCATCAACCAGCAGGCC
  ATGGACCAGCTGCATTATGTGACAGAGCTGACTGATCTTATCAAGTCAAAGTCATCACCTGATCAGAGTG
  GGATAGACGACTCTGCTAACTTTGTGGGCTTCTTTCCAACCTTTGTGTGGGCTCTGAGAGATTTCTCCCT
  GGAGTTGGAAGTCAATGGAAAACTTGTCACTCCTGATGAGTACCTGGAGCATTCGCTGACCCTGAAGAAA
  GGAGCTGATAAGAAAACTAAAAGCTTTAATGAGCCTCGACTGTGCATCAGGAAATTCTTTCCGAAGAGGA
  AGTGTTTCATCTTTGACAGACCTGCTCTGAGGAAGCAACTCTGCAAACTGGAGACTCTGGGGGAGGAGGA
  GCTGTGTAGTGAGTTTGTAGAACAAGTTGCAGAATTCACCTCGTATATCTTCAGTTACTCTGCTGTCAAA
  ACTCTGTCTGGTGGCATCATAGTCAATGGGCCACGTCTAAAGAGCCTGGTGCAGACCTATGTTGGTGCCA
  TCAGCAGTGGGTCTCTCCCCTGCATGGAGAGTGCAGTCCTGACTTTGGCCCAGATAGAGAACTCAGCTGC
  AGTACAAAAGGCCATCACCCACTATGAAGAACAGATGAATCAGAAAATCCAGATGCCCACAGAAACCCTC
  CAGGAGCTGCTAGACCTGCACAGGCTGATTGAGAGGGAGGCCATTGAGATCTTCCTAAAGAATTCTTTCA
  AGGATGTAGACCAGAAGTTCCAGACAGAATTAGGGAACCTGCTGATATCAAAGCGAGATGCCTTTATTAA
  GAAGAACTCGGATGTATCATCAGCTCATTGTTCAGATCTGATAGAGGATATTTTTGGACCTCTGGAAGAA
  GAAGTAAAACAAGGGACATTTTCCAAACCAGGAGGTTACTTCCTCTTCCTTCAGATGAGACAAGAGCTAG
  AGAAGAAGTATAACCAGGCTCCTGGGAAGGGGCTCGAGGCAGAAGCGGTGCTGAAAAAGTACTTTGAGTC
  CAAGGAAGACATTGTTGAAACACTTCTAAAGACGGATCAGTCACTCACGGAGGCAGCAAAGGAGATTGAA
  GTGGAACGGATTAAGGCTGAAACTGCTGAAGCTGCAAACAGAGAGTTAGCAGAGAAGCAGGAGAAGTTTG
  AGCTGATGATGCAGCAGAAGGAAGAGAGTTACCAGGAGCACGTGAGACAGCTGACTGAGAAGATGAAGGA
  GGAACAGAAAAAGTTAATAGAAGAACAGGACAACATCATAGCTCTTAAACTTCGGGAACAGGAAAAATTT
  CTCAGGGAAGGATACGAGAACGAGAGCAAGAAACTTCTTCGAGAGATTGAGAACATGAAGAGACGACAAT
  CACCTGGGAAATGCACCATACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214194 representing NM_133624
  Red=Cloning site Green=Tags(s)
  
  MDMASEIHMLQPMCLIENTEAHLVINQEALRILSAINQPVVVVAIVGLYRTGKSYLMNKLAGKRTGFSLG
  STVQSHTKGIWMWCVPHPKKAGQTLVLLDTEGLEDVEKGDNQNDCWIFALAVLLSSTFVYNSMGTINQQA
  MDQLHYVTELTDLIKSKSSPDQSGIDDSANFVGFFPTFVWALRDFSLELEVNGKLVTPDEYLEHSLTLKK
  GADKKTKSFNEPRLCIRKFFPKRKCFIFDRPALRKQLCKLETLGEEELCSEFVEQVAEFTSYIFSYSAVK
  TLSGGIIVNGPRLKSLVQTYVGAISSGSLPCMESAVLTLAQIENSAAVQKAITHYEEQMNQKIQMPTETL
  QELLDLHRLIEREAIEIFLKNSFKDVDQKFQTELGNLLISKRDAFIKKNSDVSSAHCSDLIEDIFGPLEE
  EVKQGTFSKPGGYFLFLQMRQELEKKYNQAPGKGLEAEAVLKKYFESKEDIVETLLKTDQSLTEAAKEIE
  VERIKAETAEAANRELAEKQEKFELMMQQKEESYQEHVRQLTEKMKEEQKKLIEEQDNIIALKLREQEKF
  LREGYENESKKLLREIENMKRRQSPGKCTIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133624
• ORF Size: 1773 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133624.2, NP_598308.1
• RefSeq Size: 2395 bp
• RefSeq ORF: 1776 bp
• Locus ID: 171164
• UniProt ID: Q63663
• Cytogenetics: 2q44
• MW: 67.4 kDa
• Gene Summary: in vitro substrate for isoprenylation by geranylgeranylpyrophosphate [RGD, Feb 2006]