Lrrc59 (NM_001008280) Rat Tagged ORF Clone

CAT#: RR214185

Lrrc59 (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 59 (Lrrc59), (10 ug)

Product Images

Circular map for RR214185

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lrrc59
• Synonyms: MGC105677
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214185 representing NM_001008280
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAAGACCGGTAGCAAGGGCGGGAACCTCCGCGACAAGCTGGACGGCAATGAGCTGGACCTGAGTC
  TCAGCGACCTGAATGAGGTCCCCGTCAAGGAGCTGGCTGCACTTCCAAAGGCCACCGTGTTGGATCTGTC
  CTGCAATAAACTGAGCACTCTTCCGTCGGATTTCTGTGGCCTCACGCACCTGGTAAAGCTGGACCTCAGC
  AAGAACAAGCTGCAGCAGCTGCCCGCAGACTTTGGTCGCCTGGTTAACCTTCAGCATTTGGATCTCCTCA
  ACAACAGGCTGGTCACCCTGCCTGTCAGCTTTGCTCAGCTCAAGAATCTGAAGTGGCTGGATCTGAAGGA
  CAATCCCTTGGATCCTGTCCTGGCCAAGGTGGCAGGTGATTGCTTGGATGAGAAGCAATGTAAGCAGTGT
  GCAAACAAGGTGTTACAGCACATGAAGGCCGTGCAGGCAGATCAGGAACGAGAGCGGCAGCGCCGGCTGG
  AAGTGGAGCGAGAGGCAGAGAAGAAGCGTGAGGCCAAGCAGCAAGCTAAGGAAGCAAAGGAGCGCGAGCT
  GAGGAAGCGGGAGAAGGCGGAGGAGAAGGAGCGTCGGCGAAAGGAGTATGATGCTCAGAAAGCTTCCAAG
  CGGGAGCAAGAGAAGAAGCCTAAGAAGGAAACAAATCAGGCCCCAAAATCGAAGTCTGGCTCTCGCCCTC
  GCAAGCCACCACCCCGAAAACACAATCGCTCCTGGGCTGTGCTGAAGGGGTTGTTGCTGCTGCTGCTGCT
  ATGTGTAGCAGGAGGGCTGGTTGTATGCCGGGTGACAGGGCTGCAACAGCAGCCCCTCTGCACCAGCGTG
  AACGCCATCTACGACAATGCCGTCCAGGGCCTGCGCCATCATGAGATCCTCCAGTGGGTCCTCCAGACCG
  ACTCCCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214185 representing NM_001008280
  Red=Cloning site Green=Tags(s)
  
  MTKTGSKGGNLRDKLDGNELDLSLSDLNEVPVKELAALPKATVLDLSCNKLSTLPSDFCGLTHLVKLDLS
  KNKLQQLPADFGRLVNLQHLDLLNNRLVTLPVSFAQLKNLKWLDLKDNPLDPVLAKVAGDCLDEKQCKQC
  ANKVLQHMKAVQADQERERQRRLEVEREAEKKREAKQQAKEAKERELRKREKAEEKERRRKEYDAQKASK
  REQEKKPKKETNQAPKSKSGSRPRKPPPRKHNRSWAVLKGLLLLLLLCVAGGLVVCRVTGLQQQPLCTSV
  NAIYDNAVQGLRHHEILQWVLQTDSQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008280
• ORF Size: 921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008280.1, NP_001008281.1
• RefSeq Size: 2825 bp
• RefSeq ORF: 924 bp
• Locus ID: 287633
• UniProt ID: Q5RJR8
• Cytogenetics: 10q26
• MW: 34.9 kDa
• Gene Summary: Required for nuclear import of FGF1, but not that of FGF2. Might regulate nuclear import of exogenous FGF1 by facilitating interaction with the nuclear import machinery and by transporting cytosolic FGF1 to, and possibly through, the nuclear pores (By similarity).[UniProtKB/Swiss-Prot Function]