Ace2 (NM_001012006) Rat Tagged ORF Clone

CAT#: RR214151

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  • TrueORF®

Ace2 (Myc-DDK-tagged ORF) - Rat angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 (Ace2), (10 ug)



  "NM_001012006" in other vectors (3)

Reconstitution Protocol

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USD 1,232.00


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Size
    • 10 ug


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Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Ace2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NM_001012006 ORF sequence, RR214151 may differ due to SNPs.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCAAGCTCCTGCTGGCTCCTTCTCAGCCTTGTTGCTGTTGCTACTGCTCAGTCCCTCATCGAGGAA
AAGGCCGAGAGCTTTTTAAACAAGTTTAACCAGGAAGCTGAAGACCTGTCTTATCAAAGTTCACTTGCT
TCTTGGAATTACAACACCAACATTACGGAGGAGAATGCCCAAAAGATGAACGAGGCTGCGGCCAAATGG
TCTGCCTTTTATGAAGAACAGTCCAAGATCGCCCAAAATTTCTCACTACAAGAAATTCAGAATGCGACC
ATCAAGCGTCAACTGAAGGCCCTTCAGCAGAGCGGGTCTTCAGCGCTGTCACCAGACAAGAACAAACAG
TTGAACACAATTCTAAACACCATGAGCACCATTTACAGTACTGGAAAAGTTTGCAACTCAATGAATCCA
CAAGAATGTTTTTTACTTGAACCAGGATTGGACGAAATAATGGCAACAAGCACAGACTACAATCGTAGG
CTCTGGGCTTGGGAGGGCTGGAGGGCTGAGGTCGGCAAGCAGCTGAGGCCGTTATATGAAGAGTATGTG
GTCCTGAAAAATGAGATGGCAAGAGCAAACAATTATGAAGACTATGGGGATTATTGGCGAGGGGATTAT
GAAGCAGAGGGAGTAGAAGGTTACAACTACAACCGAAACCAGTTGATCGAAGACGTAGAAAATACCTTC
AAAGAGATCAAACCGTTGTATGAGCAACTTCATGCCTATGTGAGAACGAAGTTGATGGAAGTGTACCCT
TCTTACATCAGCCCTACTGGATGCCTCCCTGCTCATTTGCTTGGTGATATGTGGGGTAGGTTTTGGACA
AATCTGTACCCTTTGACTACTCCCTTTCTTCAGAAACCAAACATAGATGTTACTGATGCAATGGTGAAT
CAGAGCTGGGATGCAGAAAGAATATTTAAAGAGGCAGAGAAGTTCTTCGTTTCTGTTGGCCTTCCTCAA
ATGACTCCGGGATTCTGGACAAACTCCATGCTGACTGAGCCAGGAGATGACCGGAAAGTTGTCTGCCAC
CCCACAGCTTGGGATCTGGGACATGGAGACTTCAGAATCAAGATGTGCACAAAGGTCACAATGGACAAC
TTCTTGACAGCCCATCATGAGATGGGACACATCCAATATGACATGGCATATGCCAAGCAACCTTTCCTG
CTAAGAAACGGAGCCAATGAAGGGTTCCATGAAGCCGTTGGAGAAATCATGTCACTTTCTGCAGCTACC
CCCAAACATTTGAAATCTATTGGTCTTCTGCCATCCAATTTTCAAGAAGACAATGAAACAGAAATAAAC
TTCCTACTCAAACAGGCATTGACAATTGTTGGAACGCTGCCATTTACTTACATGTTAGAGAAGTGGAGG
TGGATGGTCTTTCAGGATAAAATTCCCAGAGAACAGTGGACCAAAAAGTGGTGGGAGATGAAGCGGGAG
ATCGTTGGTGTGGTGGAGCCTCTGCCTCATGATGAAACATACTGTGACCCTGCATCTCTGTTCCATGTC
TCTAATGATTACTCATTCATTCGATATTACACAAGGACCATTTATCAATTCCAGTTTCAAGAAGCTCTT
TGTCAAGCAGCTAAACATGATGGCCCACTACACAAATGTGACATCTCAAATTCCACTGAAGCTGGGCAG
AAGTTGCTCAATATGCTGAGTCTTGGAAACTCAGGGCCCTGGACCCTAGCCTTGGAAAATGTGGTAGGA
TCAAGGAATATGGATGTAAAACCACTGCTCAATTACTTCCAACCATTGTTTGTCTGGCTGAAAGAGCAG
AACAGGAATTCGACTGTGGGGTGGAGCACTGACTGGAGCCCATATGCCGACCAAAGCATTAAAGTGAGG
ATAAGCCTAAAATCAGCTCTTGGGAAAAATGCGTATGAATGGACCGACAACGAAATGTACCTATTCCGA
TCATCTGTTGCCTATGCCATGAGAGAGTATTTTTCAAGGGAAAAGAACCAGACAGTTCCTTTTGGGGAG
GCAGACGTATGGGTGAGTGATTTGAAACCAAGAGTCTCCTTCAACTTCTTTGTCACTTCACCCAAAAAT
GTGTCTGACATCATTCCCAGAAGTGAAGTTGAAGAGGCCATCAGGATGTCTCGGGGCCGTATCAATGAT
ATTTTTGGTCTGAATGATAACAGCCTGGAGTTTCTGGGGATCTACCCAACACTTAAGCCACCTTACGAG
CCTCCTGTCACCATATGGCTGATTATTTTTGGTGTCGTGATGGGAACGGTAGTGGTTGGCATTGTTATC
CTGATCGTCACTGGGATCAAAGGTCGAAAGAAGAAAAATGAAACAAAAAGAGAAGAGAATCCTTATGAC
TCCATGGACATTGGCAAAGGAGAAAGTAACGCAGGATTCCAAAACAGTGATGATGCTCAAACTTCATTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>Peptide sequence encoded by RR214151
Blue=ORF Red=Cloning site Green=Tag(s)

MSSSCWLLLSLVAVATAQSLIEEKAESFLNKFNQEAEDLSYQSSLASWNYNTNITEENAQKMNEAAAKW
SAFYEEQSKIAQNFSLQEIQNATIKRQLKALQQSGSSALSPDKNKQLNTILNTMSTIYSTGKVCNSMNP
QECFLLEPGLDEIMATSTDYNRRLWAWEGWRAEVGKQLRPLYEEYVVLKNEMARANNYEDYGDYWRGDY
EAEGVEGYNYNRNQLIEDVENTFKEIKPLYEQLHAYVRTKLMEVYPSYISPTGCLPAHLLGDMWGRFWT
NLYPLTTPFLQKPNIDVTDAMVNQSWDAERIFKEAEKFFVSVGLPQMTPGFWTNSMLTEPGDDRKVVCH
PTAWDLGHGDFRIKMCTKVTMDNFLTAHHEMGHIQYDMAYAKQPFLLRNGANEGFHEAVGEIMSLSAAT
PKHLKSIGLLPSNFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFQDKIPREQWTKKWWEMKRE
IVGVVEPLPHDETYCDPASLFHVSNDYSFIRYYTRTIYQFQFQEALCQAAKHDGPLHKCDISNSTEAGQ
KLLNMLSLGNSGPWTLALENVVGSRNMDVKPLLNYFQPLFVWLKEQNRNSTVGWSTDWSPYADQSIKVR
ISLKSALGKNAYEWTDNEMYLFRSSVAYAMREYFSREKNQTVPFGEADVWVSDLKPRVSFNFFVTSPKN
VSDIIPRSEVEEAIRMSRGRINDIFGLNDNSLEFLGIYPTLKPPYEPPVTIWLIIFGVVMGTVVVGIVI
LIVTGIKGRKKKNETKREENPYDSMDIGKGESNAGFQNSDDAQTSF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001012006
ORF Size 2415 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001012006.1, NP_001012006.1
RefSeq Size 3268 bp
RefSeq ORF 2418 bp
Locus ID 302668
UniProt ID Q5EGZ1
Cytogenetics Xq14
MW 92.5 kDa
Gene Summary enzyme peptidyl-dipeptidase; mouse homolog has a role in regulation of cardiac contractility and regulating Angiotensin 1 and 2 levels [RGD, Feb 2006]

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