Ace2 (NM_001012006) Rat Tagged ORF Clone

CAT#: RR214151

Ace2 (Myc-DDK-tagged ORF) - Rat angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 (Ace2), (10 ug)

Product Images

Circular map for RR214151

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ace2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >NM_001012006 ORF sequence, RR214151 may differ due to SNPs.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCAAGCTCCTGCTGGCTCCTTCTCAGCCTTGTTGCTGTTGCTACTGCTCAGTCCCTCATCGAGGAA
  AAGGCCGAGAGCTTTTTAAACAAGTTTAACCAGGAAGCTGAAGACCTGTCTTATCAAAGTTCACTTGCT
  TCTTGGAATTACAACACCAACATTACGGAGGAGAATGCCCAAAAGATGAACGAGGCTGCGGCCAAATGG
  TCTGCCTTTTATGAAGAACAGTCCAAGATCGCCCAAAATTTCTCACTACAAGAAATTCAGAATGCGACC
  ATCAAGCGTCAACTGAAGGCCCTTCAGCAGAGCGGGTCTTCAGCGCTGTCACCAGACAAGAACAAACAG
  TTGAACACAATTCTAAACACCATGAGCACCATTTACAGTACTGGAAAAGTTTGCAACTCAATGAATCCA
  CAAGAATGTTTTTTACTTGAACCAGGATTGGACGAAATAATGGCAACAAGCACAGACTACAATCGTAGG
  CTCTGGGCTTGGGAGGGCTGGAGGGCTGAGGTCGGCAAGCAGCTGAGGCCGTTATATGAAGAGTATGTG
  GTCCTGAAAAATGAGATGGCAAGAGCAAACAATTATGAAGACTATGGGGATTATTGGCGAGGGGATTAT
  GAAGCAGAGGGAGTAGAAGGTTACAACTACAACCGAAACCAGTTGATCGAAGACGTAGAAAATACCTTC
  AAAGAGATCAAACCGTTGTATGAGCAACTTCATGCCTATGTGAGAACGAAGTTGATGGAAGTGTACCCT
  TCTTACATCAGCCCTACTGGATGCCTCCCTGCTCATTTGCTTGGTGATATGTGGGGTAGGTTTTGGACA
  AATCTGTACCCTTTGACTACTCCCTTTCTTCAGAAACCAAACATAGATGTTACTGATGCAATGGTGAAT
  CAGAGCTGGGATGCAGAAAGAATATTTAAAGAGGCAGAGAAGTTCTTCGTTTCTGTTGGCCTTCCTCAA
  ATGACTCCGGGATTCTGGACAAACTCCATGCTGACTGAGCCAGGAGATGACCGGAAAGTTGTCTGCCAC
  CCCACAGCTTGGGATCTGGGACATGGAGACTTCAGAATCAAGATGTGCACAAAGGTCACAATGGACAAC
  TTCTTGACAGCCCATCATGAGATGGGACACATCCAATATGACATGGCATATGCCAAGCAACCTTTCCTG
  CTAAGAAACGGAGCCAATGAAGGGTTCCATGAAGCCGTTGGAGAAATCATGTCACTTTCTGCAGCTACC
  CCCAAACATTTGAAATCTATTGGTCTTCTGCCATCCAATTTTCAAGAAGACAATGAAACAGAAATAAAC
  TTCCTACTCAAACAGGCATTGACAATTGTTGGAACGCTGCCATTTACTTACATGTTAGAGAAGTGGAGG
  TGGATGGTCTTTCAGGATAAAATTCCCAGAGAACAGTGGACCAAAAAGTGGTGGGAGATGAAGCGGGAG
  ATCGTTGGTGTGGTGGAGCCTCTGCCTCATGATGAAACATACTGTGACCCTGCATCTCTGTTCCATGTC
  TCTAATGATTACTCATTCATTCGATATTACACAAGGACCATTTATCAATTCCAGTTTCAAGAAGCTCTT
  TGTCAAGCAGCTAAACATGATGGCCCACTACACAAATGTGACATCTCAAATTCCACTGAAGCTGGGCAG
  AAGTTGCTCAATATGCTGAGTCTTGGAAACTCAGGGCCCTGGACCCTAGCCTTGGAAAATGTGGTAGGA
  TCAAGGAATATGGATGTAAAACCACTGCTCAATTACTTCCAACCATTGTTTGTCTGGCTGAAAGAGCAG
  AACAGGAATTCGACTGTGGGGTGGAGCACTGACTGGAGCCCATATGCCGACCAAAGCATTAAAGTGAGG
  ATAAGCCTAAAATCAGCTCTTGGGAAAAATGCGTATGAATGGACCGACAACGAAATGTACCTATTCCGA
  TCATCTGTTGCCTATGCCATGAGAGAGTATTTTTCAAGGGAAAAGAACCAGACAGTTCCTTTTGGGGAG
  GCAGACGTATGGGTGAGTGATTTGAAACCAAGAGTCTCCTTCAACTTCTTTGTCACTTCACCCAAAAAT
  GTGTCTGACATCATTCCCAGAAGTGAAGTTGAAGAGGCCATCAGGATGTCTCGGGGCCGTATCAATGAT
  ATTTTTGGTCTGAATGATAACAGCCTGGAGTTTCTGGGGATCTACCCAACACTTAAGCCACCTTACGAG
  CCTCCTGTCACCATATGGCTGATTATTTTTGGTGTCGTGATGGGAACGGTAGTGGTTGGCATTGTTATC
  CTGATCGTCACTGGGATCAAAGGTCGAAAGAAGAAAAATGAAACAAAAAGAGAAGAGAATCCTTATGAC
  TCCATGGACATTGGCAAAGGAGAAAGTAACGCAGGATTCCAAAACAGTGATGATGCTCAAACTTCATTC
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RR214151
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSSSCWLLLSLVAVATAQSLIEEKAESFLNKFNQEAEDLSYQSSLASWNYNTNITEENAQKMNEAAAKW
  SAFYEEQSKIAQNFSLQEIQNATIKRQLKALQQSGSSALSPDKNKQLNTILNTMSTIYSTGKVCNSMNP
  QECFLLEPGLDEIMATSTDYNRRLWAWEGWRAEVGKQLRPLYEEYVVLKNEMARANNYEDYGDYWRGDY
  EAEGVEGYNYNRNQLIEDVENTFKEIKPLYEQLHAYVRTKLMEVYPSYISPTGCLPAHLLGDMWGRFWT
  NLYPLTTPFLQKPNIDVTDAMVNQSWDAERIFKEAEKFFVSVGLPQMTPGFWTNSMLTEPGDDRKVVCH
  PTAWDLGHGDFRIKMCTKVTMDNFLTAHHEMGHIQYDMAYAKQPFLLRNGANEGFHEAVGEIMSLSAAT
  PKHLKSIGLLPSNFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFQDKIPREQWTKKWWEMKRE
  IVGVVEPLPHDETYCDPASLFHVSNDYSFIRYYTRTIYQFQFQEALCQAAKHDGPLHKCDISNSTEAGQ
  KLLNMLSLGNSGPWTLALENVVGSRNMDVKPLLNYFQPLFVWLKEQNRNSTVGWSTDWSPYADQSIKVR
  ISLKSALGKNAYEWTDNEMYLFRSSVAYAMREYFSREKNQTVPFGEADVWVSDLKPRVSFNFFVTSPKN
  VSDIIPRSEVEEAIRMSRGRINDIFGLNDNSLEFLGIYPTLKPPYEPPVTIWLIIFGVVMGTVVVGIVI
  LIVTGIKGRKKKNETKREENPYDSMDIGKGESNAGFQNSDDAQTSF
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012006
• ORF Size: 2415 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012006.1, NP_001012006.1
• RefSeq Size: 3268 bp
• RefSeq ORF: 2418 bp
• Locus ID: 302668
• UniProt ID: Q5EGZ1
• Cytogenetics: Xq14
• MW: 92.5 kDa
• Gene Summary: enzyme peptidyl-dipeptidase; mouse homolog has a role in regulation of cardiac contractility and regulating Angiotensin 1 and 2 levels [RGD, Feb 2006]