Capns1 (NM_017118) Rat Tagged ORF Clone

CAT#: RR214051

Capns1 (Myc-DDK-tagged ORF) - Rat calpain, small subunit 1 (Capns1), (10 ug)

Product Images

Circular map for RR214051

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Capns1
• Synonyms: Capn4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214051 representing NM_017118
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTTGGTGAATTCGTTCTTGAAGGGCGGCGGTGGCGGTGGGGGAGGCGGGGGTCTGGGCGGGGGCC
  TGGGCAATGTGCTCGGAGGCCTGATCAGCGGAGCTGCTGGAGGCGGCGGCGGTGGAGGCGGCGGTGGCGG
  CATGGGACTGGGAGGAGGTGGCGGAGGCGGTGGAACAGCCATGCGTATCCTGGGCGGAGTCATTAGCGCC
  ATCAGCGAGGCTGCTGCGCAGTACAACCCGGAGCCCCCGCCCCCACGTAGCCATTACTCTAATATTGAGG
  CCAACGAGAGTGAAGAGGAACGTCAGTTCAGGAAACTTTTTGTCCAGCTGGCTGGAGACGACATGGAGGT
  CAGCGCTACAGAACTCATGAATATCCTCAACAAGGTCGTGACCCGACACCCAGATCTGAAAACTGATGGG
  TTCGGCATTGACACTTGTCGGAGCATGGTGGCTGTTATGGATAGTGACACCACAGGCAAGCTGGGCTTTG
  AGGAGTTCAAGTACCTCTGGAACAACATTAAAAAGTGGCAGGGTATATACAAACGCTTTGATACTGACCG
  ATCAGGGACCATTGGTAGCAATGAACTCCCAGGGGCCTTTGAGGCAGCAGGATTCCACCTGAATCAGCAT
  ATCTACAGCATGATCATCCGCCGCTACTCGGACGAGACCGGGAACATGGATTTCGATAACTTCATCAGCT
  GCTTGGTCAGGCTGGATGCCATGTTCCGTGCTTTCAGATCTCTTGACAAAAATGGCACTGGACAGATCCA
  AGTGAACATCCAGGAGTGGTTGCAGCTGACTATGTATTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214051 representing NM_017118
  Red=Cloning site Green=Tags(s)
  
  MFLVNSFLKGGGGGGGGGGLGGGLGNVLGGLISGAAGGGGGGGGGGGMGLGGGGGGGGTAMRILGGVISA
  ISEAAAQYNPEPPPPRSHYSNIEANESEEERQFRKLFVQLAGDDMEVSATELMNILNKVVTRHPDLKTDG
  FGIDTCRSMVAVMDSDTTGKLGFEEFKYLWNNIKKWQGIYKRFDTDRSGTIGSNELPGAFEAAGFHLNQH
  IYSMIIRRYSDETGNMDFDNFISCLVRLDAMFRAFRSLDKNGTGQIQVNIQEWLQLTMYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017118
• ORF Size: 810 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017118.1, NP_058814.1
• RefSeq Size: 1405 bp
• RefSeq ORF: 813 bp
• Locus ID: 29156
• UniProt ID: Q64537
• Cytogenetics: 1q21
• MW: 28.6 kDa
• Gene Summary: small subunit of calcium-activated protease calpain; functions only in conjunction with large subunit [RGD, Feb 2006]